Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.
Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.Show MeSH
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Mentions: The number of neutrophil in the BALF and lung MPO activity were significantly increased in rats ventilated with HVZP than other animals (P < 0.05, Fig.2A and B). Meanwhile, KGF-2 pre-treatment significantly decreased neutrophil counts and MPO activity (P < 0.05, Fig.2A and B). Both TNF-α and MIP-2 in the BALF were significantly increased in HVZP group compared with those in the control group (P < 0.05, Fig.2C and D). Keratinocyte growth factor-2 pre-treatment significantly decreased TNF-α and MIP-2 in the BALF compared with those in the HVZP group (P < 0.05, Fig.2C and D).
Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.