Limits...
Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.

Bi J, Tong L, Zhu X, Yang D, Bai C, Song Y, She J - J. Cell. Mol. Med. (2014)

Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Show MeSH

Related in: MedlinePlus

KGF-2 improves HVZP-induced hypoxaemia and protein-rich oedema. (A) Partial pressure of oxygen (PaO2) after 4 hrs mechanical ventilation. PaO2 in HVZP group was significantly lower than that in the control group (P < 0.05). KGF-2 pre-treatment significantly increased PaO2 compared with the HVZP groups (P < 0.05). (B) PaCO2 after 4 hrs mechanical ventilation. PaCO2 in HVZP group was significantly higher than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (C) pH value after 4 hrs mechanical ventilation. pH value in HVZP group was significantly lower than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (D) Lung W/D weight ratios. W/D ratio in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (E) The number of total cells recovered in the bronchoalveolar lavage fluid (BALF). Total Cell Count in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (F) The total protein contents recovered in the bronchoalveolar lavage fluid. Total protein contents in HVZP group were significantly higher than those in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). No statistical difference was discovered between KGF-2 and control groups. *P < 0.05 versus control group; †P < 0.05 versusHVZP group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4508161&req=5

fig01: KGF-2 improves HVZP-induced hypoxaemia and protein-rich oedema. (A) Partial pressure of oxygen (PaO2) after 4 hrs mechanical ventilation. PaO2 in HVZP group was significantly lower than that in the control group (P < 0.05). KGF-2 pre-treatment significantly increased PaO2 compared with the HVZP groups (P < 0.05). (B) PaCO2 after 4 hrs mechanical ventilation. PaCO2 in HVZP group was significantly higher than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (C) pH value after 4 hrs mechanical ventilation. pH value in HVZP group was significantly lower than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (D) Lung W/D weight ratios. W/D ratio in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (E) The number of total cells recovered in the bronchoalveolar lavage fluid (BALF). Total Cell Count in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (F) The total protein contents recovered in the bronchoalveolar lavage fluid. Total protein contents in HVZP group were significantly higher than those in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). No statistical difference was discovered between KGF-2 and control groups. *P < 0.05 versus control group; †P < 0.05 versusHVZP group.

Mentions: After 4 hrs MV, the partial pressure of oxygen (PaO2) of rats in HVZP group was significantly lower than that in control group (P < 0.05, Fig.1A). KGF-2 pre-treatment significantly increased PaO2 compared with the HVZP groups (P < 0.05, Fig.1A). In addition, PH value was significantly lower and the partial pressure of carbon dioxide (PaCO2) was significantly higher in animals receiving high VT (P < 0.05, Fig.1B and C), but values were similar between those pre-treated with KGF-2 and PBS.


Keratinocyte growth factor-2 intratracheal instillation significantly attenuates ventilator-induced lung injury in rats.

Bi J, Tong L, Zhu X, Yang D, Bai C, Song Y, She J - J. Cell. Mol. Med. (2014)

KGF-2 improves HVZP-induced hypoxaemia and protein-rich oedema. (A) Partial pressure of oxygen (PaO2) after 4 hrs mechanical ventilation. PaO2 in HVZP group was significantly lower than that in the control group (P < 0.05). KGF-2 pre-treatment significantly increased PaO2 compared with the HVZP groups (P < 0.05). (B) PaCO2 after 4 hrs mechanical ventilation. PaCO2 in HVZP group was significantly higher than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (C) pH value after 4 hrs mechanical ventilation. pH value in HVZP group was significantly lower than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (D) Lung W/D weight ratios. W/D ratio in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (E) The number of total cells recovered in the bronchoalveolar lavage fluid (BALF). Total Cell Count in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (F) The total protein contents recovered in the bronchoalveolar lavage fluid. Total protein contents in HVZP group were significantly higher than those in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). No statistical difference was discovered between KGF-2 and control groups. *P < 0.05 versus control group; †P < 0.05 versusHVZP group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508161&req=5

fig01: KGF-2 improves HVZP-induced hypoxaemia and protein-rich oedema. (A) Partial pressure of oxygen (PaO2) after 4 hrs mechanical ventilation. PaO2 in HVZP group was significantly lower than that in the control group (P < 0.05). KGF-2 pre-treatment significantly increased PaO2 compared with the HVZP groups (P < 0.05). (B) PaCO2 after 4 hrs mechanical ventilation. PaCO2 in HVZP group was significantly higher than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (C) pH value after 4 hrs mechanical ventilation. pH value in HVZP group was significantly lower than that in the control group (P < 0.05). No statistical difference was discovered between HVZP group and HVZP+KGF-2 group. (D) Lung W/D weight ratios. W/D ratio in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (E) The number of total cells recovered in the bronchoalveolar lavage fluid (BALF). Total Cell Count in HVZP group was significantly higher than that in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). (F) The total protein contents recovered in the bronchoalveolar lavage fluid. Total protein contents in HVZP group were significantly higher than those in control group (P < 0.05) and HVZP+KGF-2 group (P < 0.05). No statistical difference was discovered between KGF-2 and control groups. *P < 0.05 versus control group; †P < 0.05 versusHVZP group.
Mentions: After 4 hrs MV, the partial pressure of oxygen (PaO2) of rats in HVZP group was significantly lower than that in control group (P < 0.05, Fig.1A). KGF-2 pre-treatment significantly increased PaO2 compared with the HVZP groups (P < 0.05, Fig.1A). In addition, PH value was significantly lower and the partial pressure of carbon dioxide (PaCO2) was significantly higher in animals receiving high VT (P < 0.05, Fig.1B and C), but values were similar between those pre-treated with KGF-2 and PBS.

Bottom Line: Inflammatory cytokines (tumour necrosis factor-α, macrophage inflammatory protein 2), neutrophil and total protein levels in bronchoalveolar lavage fluid and surfactant protein mRNA expression in lung tissue were detected; the number of alveolar type II cells, lung water content and lung morphology were also evaluated.The results indicate that pre-treatment with KGF-2 showed dramatic improvement in lung oedema and inflammation compared with HVZP alone, together with increased surfactant protein mRNA and alveolar type II cells.Our results suggest that KGF-2 might be considered a promising prevention for human VILI or other acute lung injury diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus