XBP1S, a BMP2-inducible transcription factor, accelerates endochondral bone growth by activating GEP growth factor.
Bottom Line: XBP1S stimulates chondrocyte differentiation from mesenchymal stem cells in vitro and endochondral ossification ex vivo.Furthermore, XBP1S enhances GEP-stimulated chondrogenesis and endochondral bone formation.Collectively, these findings demonstrate that XBP1S, a BMP2-inducible transcription factor, positively regulates endochondral bone formation by activating GEP chondrogenic growth factor.
Affiliation: Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, China.Show MeSH
Related in: MedlinePlus
Mentions: Foetal mouse metatarsals were dissected from foetal GEP mice (GEP−/−, 15-day-old embryos) and cultured in DMEM (Gibco, Carlsbad, CA, USA) containing 1% heat-inactivated foetal calf serum (Invitrogen) and 100 U penicillin-streptomycin per milliliter in the absence or presence of various stimuli for 5 days, as indicated in Figures4, 8 and 9.
Affiliation: Department of Cell Biology and Genetics, Core Facility of Development Biology, Chongqing Medical University, Chongqing, China.