Renal telocytes contribute to the repair of ischemically injured renal tubules.
Bottom Line: In a renal IRI model, transplantation of renal TCs was found to decrease serum creatinine and blood urea nitrogen (BUN) levels, while renal fibroblasts exerted no such effect.Our data suggest that the protective effect of TCs against IRI occurs via inflammation-independent mechanisms in vivo.TCs did not display any advantage in paracrine growth factor secretion in vitro compared with renal fibroblasts.
Affiliation: Department of Urology, Fudan University Zhongshan Hospital, Shanghai, China; Shanghai Key Lab of Organ Transplantation, Shanghai, China.Show MeSH
Related in: MedlinePlus
Mentions: The rats subjected to renal IRI exhibited a significant increase in Scr (421.75 ± 102.81 μmol/l) and BUN (43.25 ± 3.40 mmol/l) levels compared with the sham-operated rats (51.50 ± 11.47 μmol/l and 5.20 ± 0.43 mmol/l respectively). Transplantation of renal TCs decreased Scr and BUN levels, while transplantation of renal fibroblasts had no such effect (Fig.3A and B, Table3). Following IRI, TEC necrosis and tubular injuries were clear (Fig.2C). These injuries included loss of the brush border and dilation of the renal tubules and urinary cylinder. As previously mentioned, the assessment of histological injury was performed with a scoring system ranging from 0 to 3 points. The TC-injected groups presented a lower score compared with the fibroblast-injected and PBS-injected groups (Fig.3D and Table4). Renal TC transplantation also resulted in reduced expression of cleaved caspase-3 compared with the injection of both renal fibroblasts and PBS (Fig.3E), which suggests that renal TCs could inhibit TEC apoptosis after IRI.
Affiliation: Department of Urology, Fudan University Zhongshan Hospital, Shanghai, China; Shanghai Key Lab of Organ Transplantation, Shanghai, China.