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Renal telocytes contribute to the repair of ischemically injured renal tubules.

Li L, Lin M, Li L, Wang R, Zhang C, Qi G, Xu M, Rong R, Zhu T - J. Cell. Mol. Med. (2014)

Bottom Line: However, their precise function in organ regeneration remains unknown.The results of histological injury assessments and the expression levels of cleaved caspase-3 were consistent with a change in kidney function.Our data suggest that the protective effect of TCs against IRI occurs via inflammation-independent mechanisms in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Zhongshan Hospital, Shanghai, China; Shanghai Key Lab of Organ Transplantation, Shanghai, China.

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Effects of renal telocytes (TCs) on renal function, histological changes and the expression of cleaved caspase-3 at 48 hrs after renal ischaemia–reperfusion injury (IRI). Scr (A) and BUN (B) levels were increased following renal IRI. Renal TC transplantation prevented renal dysfunction, while FIB injection had no effect after renal IRI. The histological damage (C) following IRI was also ameliorated by TC injection based on a semi-quantitative assessment (D). The expression of cleaved caspase-3 was decreased after renal TC transplantation compared with the expression in the PBS injected and FIB injected groups (E). * Statistically significant difference compared with the sham group; # statistically significant difference between the TC-injected group and the PBS-injected group; $ statistically significant difference between the TC-injected group and the FIB-injected group. *P < 0.05, **P < 0.01, ***P < 0.001.
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fig03: Effects of renal telocytes (TCs) on renal function, histological changes and the expression of cleaved caspase-3 at 48 hrs after renal ischaemia–reperfusion injury (IRI). Scr (A) and BUN (B) levels were increased following renal IRI. Renal TC transplantation prevented renal dysfunction, while FIB injection had no effect after renal IRI. The histological damage (C) following IRI was also ameliorated by TC injection based on a semi-quantitative assessment (D). The expression of cleaved caspase-3 was decreased after renal TC transplantation compared with the expression in the PBS injected and FIB injected groups (E). * Statistically significant difference compared with the sham group; # statistically significant difference between the TC-injected group and the PBS-injected group; $ statistically significant difference between the TC-injected group and the FIB-injected group. *P < 0.05, **P < 0.01, ***P < 0.001.

Mentions: The rats subjected to renal IRI exhibited a significant increase in Scr (421.75 ± 102.81 μmol/l) and BUN (43.25 ± 3.40 mmol/l) levels compared with the sham-operated rats (51.50 ± 11.47 μmol/l and 5.20 ± 0.43 mmol/l respectively). Transplantation of renal TCs decreased Scr and BUN levels, while transplantation of renal fibroblasts had no such effect (Fig.3A and B, Table3). Following IRI, TEC necrosis and tubular injuries were clear (Fig.2C). These injuries included loss of the brush border and dilation of the renal tubules and urinary cylinder. As previously mentioned, the assessment of histological injury was performed with a scoring system ranging from 0 to 3 points. The TC-injected groups presented a lower score compared with the fibroblast-injected and PBS-injected groups (Fig.3D and Table4). Renal TC transplantation also resulted in reduced expression of cleaved caspase-3 compared with the injection of both renal fibroblasts and PBS (Fig.3E), which suggests that renal TCs could inhibit TEC apoptosis after IRI.


Renal telocytes contribute to the repair of ischemically injured renal tubules.

Li L, Lin M, Li L, Wang R, Zhang C, Qi G, Xu M, Rong R, Zhu T - J. Cell. Mol. Med. (2014)

Effects of renal telocytes (TCs) on renal function, histological changes and the expression of cleaved caspase-3 at 48 hrs after renal ischaemia–reperfusion injury (IRI). Scr (A) and BUN (B) levels were increased following renal IRI. Renal TC transplantation prevented renal dysfunction, while FIB injection had no effect after renal IRI. The histological damage (C) following IRI was also ameliorated by TC injection based on a semi-quantitative assessment (D). The expression of cleaved caspase-3 was decreased after renal TC transplantation compared with the expression in the PBS injected and FIB injected groups (E). * Statistically significant difference compared with the sham group; # statistically significant difference between the TC-injected group and the PBS-injected group; $ statistically significant difference between the TC-injected group and the FIB-injected group. *P < 0.05, **P < 0.01, ***P < 0.001.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4508154&req=5

fig03: Effects of renal telocytes (TCs) on renal function, histological changes and the expression of cleaved caspase-3 at 48 hrs after renal ischaemia–reperfusion injury (IRI). Scr (A) and BUN (B) levels were increased following renal IRI. Renal TC transplantation prevented renal dysfunction, while FIB injection had no effect after renal IRI. The histological damage (C) following IRI was also ameliorated by TC injection based on a semi-quantitative assessment (D). The expression of cleaved caspase-3 was decreased after renal TC transplantation compared with the expression in the PBS injected and FIB injected groups (E). * Statistically significant difference compared with the sham group; # statistically significant difference between the TC-injected group and the PBS-injected group; $ statistically significant difference between the TC-injected group and the FIB-injected group. *P < 0.05, **P < 0.01, ***P < 0.001.
Mentions: The rats subjected to renal IRI exhibited a significant increase in Scr (421.75 ± 102.81 μmol/l) and BUN (43.25 ± 3.40 mmol/l) levels compared with the sham-operated rats (51.50 ± 11.47 μmol/l and 5.20 ± 0.43 mmol/l respectively). Transplantation of renal TCs decreased Scr and BUN levels, while transplantation of renal fibroblasts had no such effect (Fig.3A and B, Table3). Following IRI, TEC necrosis and tubular injuries were clear (Fig.2C). These injuries included loss of the brush border and dilation of the renal tubules and urinary cylinder. As previously mentioned, the assessment of histological injury was performed with a scoring system ranging from 0 to 3 points. The TC-injected groups presented a lower score compared with the fibroblast-injected and PBS-injected groups (Fig.3D and Table4). Renal TC transplantation also resulted in reduced expression of cleaved caspase-3 compared with the injection of both renal fibroblasts and PBS (Fig.3E), which suggests that renal TCs could inhibit TEC apoptosis after IRI.

Bottom Line: However, their precise function in organ regeneration remains unknown.The results of histological injury assessments and the expression levels of cleaved caspase-3 were consistent with a change in kidney function.Our data suggest that the protective effect of TCs against IRI occurs via inflammation-independent mechanisms in vivo.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Fudan University Zhongshan Hospital, Shanghai, China; Shanghai Key Lab of Organ Transplantation, Shanghai, China.

Show MeSH
Related in: MedlinePlus