Renal telocytes contribute to the repair of ischemically injured renal tubules.
Bottom Line: In a renal IRI model, transplantation of renal TCs was found to decrease serum creatinine and blood urea nitrogen (BUN) levels, while renal fibroblasts exerted no such effect.Our data suggest that the protective effect of TCs against IRI occurs via inflammation-independent mechanisms in vivo.TCs did not display any advantage in paracrine growth factor secretion in vitro compared with renal fibroblasts.
Affiliation: Department of Urology, Fudan University Zhongshan Hospital, Shanghai, China; Shanghai Key Lab of Organ Transplantation, Shanghai, China.Show MeSH
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Mentions: Based on phase-contrast microscopy, the primary culture of isolated CD117+ renal TCs displayed renal TCs with piriform/spindle/triangular cell bodies containing long, slender Tps, showing an alternation of thick segments (podoms) and thin segments (podomers; Fig.1A). The renal TCs with this unique morphology were positive for both CD117 and CD34 (Fig.1B–D). The purity of the isolated renal TCs was determined based on double immunofluorescence staining of CD117 and CD34. Approximately 95.50 ± 1.76% of the total cells were CD117-positive, while approximately 97.25 ± 2.33% were CD34-positive and ∽93.38 ± 3.11% were both CD117- and CD34-positive (Fig.1E and Table2).
Affiliation: Department of Urology, Fudan University Zhongshan Hospital, Shanghai, China; Shanghai Key Lab of Organ Transplantation, Shanghai, China.