Retinoic acid reduces migration of human breast cancer cells: role of retinoic acid receptor beta.
Bottom Line: The retinoic acid receptor β (RARβ) is not expressed in 50% of invasive breast carcinoma compared with normal tissue and it has been associated with lymph node metastasis.On the contrary, the addition of the selective retinoid RARβ-agonist (BMS453) significantly reduced cell migration comparable to RA inhibition.When RARβ gene silencing was performed, the RA failed to significantly inhibit migration and resulted ineffective to reduce moesin, c-Src and FAK expressions.
Affiliation: Tumor Biology Laboratory, Institute of Medicine and Experimental Biology of Cuyo, National Research Council of Argentina, Mendoza, Argentina.Show MeSH
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Mentions: The effect of RA on breast cancer cell migration was then tested in a dose–response experiment. To distinguish cell migration from cell proliferation, Cytosine-β-d-arabinofuranoside hydrochloride (10 μM), a selective inhibitor of DNA strand separation that does not block RNA synthesis, was used to arrest cell proliferation. After partially scraping out MCF7 cells from the cell culture dish, we monitored the movement of the remaining cells for the following 72 hrs. After 72 hrs, 10−6 and 10−5 M of RA significantly inhibited the migration of MCF7 cells towards the scraped area ‘the wound healing’ compared with control untreated cells (Fig.2A and B). It is important to note that the 60% of cell migration inhibition started from RA 10−6 M, but at the same concentration the cell viability was not affected (Figs1A and 2A, B). Similar results were obtained in T47D cellular line (data not shown).
Affiliation: Tumor Biology Laboratory, Institute of Medicine and Experimental Biology of Cuyo, National Research Council of Argentina, Mendoza, Argentina.