Protein profiling of human lung telocytes and microvascular endothelial cells using iTRAQ quantitative proteomics.
Bottom Line: Bioinformatics analysis using Panther revealed that the 38 proteins associated with TCs represented cellular functions such as intercellular communication (via vesicle mediated transport) and structure morphogenesis, being mainly cytoskeletal proteins and oxidoreductases.In conclusion, we report here the first extensive comparison of proteins from TCs and ECs using a quantitative proteomics approach.Moreover, they might inhibit the oxidative stress and cellular ageing and may have pro-proliferative effects through the inhibition of apoptosis.
Affiliation: Fudan University Center for Clinical Bioinformatics, Zhongshan Hospital, Fudan University School of Medicine, Shanghai, China.Show MeSH
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Mentions: Figures5–7 show the distributions of differentially proteins in putative functional categories. The highly expressed proteins in TCs are involved in important molecular functions such as: catalytic activity (15 proteins), structural molecule activity (10 proteins), binding (5 proteins), receptor activity (3 proteins), transporter activity (2 proteins) as seen in Figure5A compared to ECs where significantly more proteins are involved in binding (24 proteins), structural molecule activity (21 proteins), catalytic activity (20 proteins), nucleic acid-binding transcription (6 proteins), enzyme regulator activity (3 proteins), anti-oxidant activity (1 protein) - Figure5B.
Affiliation: Fudan University Center for Clinical Bioinformatics, Zhongshan Hospital, Fudan University School of Medicine, Shanghai, China.