Protective role of 5-azacytidine on myocardial infarction is associated with modulation of macrophage phenotype and inhibition of fibrosis.
Bottom Line: Nitric oxide was produced by PGN, which was reduced by 77.87% after 5AZ treatment.Cardiac fibrosis in the MI+5AZ was 8.14 ± 1.00%, compared with 14.93 ± 2.98% in the MI group (P < 0.05).In cardiac fibroblasts, pro-fibrotic mediators and cell proliferation were increased by AngII, and these increases were attenuated after 5AZ treatment. 5AZ exerts its cardiac protective role through modulation of macrophages and cardiac fibroblasts.
Affiliation: Heart Research Center, Chonnam National University Hospital, Gwangju, South Korea.Show MeSH
Related in: MedlinePlus
Mentions: To investigate the role of 5AZ in MI, MI was induced by permanent ligation of the left anterior descending coronary artery in rats. To confirm whether the surgery was induced similar MI in all animals, infarct size was assessed at 24 hrs. Infarct size was similar in the two groups (Fig.4A). Then, 5AZ or saline was administered, and heart tissue was harvested 2 weeks after MI. Cardiac fibrosis was reduced to 8.14 ± 1.00% by 5AZ administration from 14.93 ± 2.98% (Fig.4B).
Affiliation: Heart Research Center, Chonnam National University Hospital, Gwangju, South Korea.