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Comprehensive characterization of immunoglobulin gene rearrangements in patients with chronic lymphocytic leukaemia.

René C, Prat N, Thuizat A, Broctawik M, Avinens O, Eliaou JF - J. Cell. Mol. Med. (2014)

Bottom Line: Described biased usage of variable, diversity and joining genes between the mutated and unmutated groups was found in our population.Surprisingly, localization of activation-induced cytidine deaminase motifs onto the variable gene showed a preference for framework regions.The study of the characteristics at the age of diagnosis showed no difference in clinical outcome, but suggested a tendency of increased replacement and transition-over-transversion mutations and a longer third CDR length in older patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, CHRU de Montpellier, University Hospital Saint-Eloi, Montpellier, France; Faculté de Médecine, University of Montpellier 1, Montpellier, France.

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Comparison of mutational rate according to the set of primers used. The IGVH mutation percentage was determined by either an entire IGVH sequencing with IGHV-Leader primers (x axes) or partial sequencing with IGHV-FR1 primers (y axes). The percentage was obtained by dividing the number of mutations by the length (in nucleotides) of each region. One plot corresponds to one patient. Comparisons were performed in 64 patients by a Spearman's correlation test (correlation coefficient R2 = 0.97, P < 0.0001).
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fig01: Comparison of mutational rate according to the set of primers used. The IGVH mutation percentage was determined by either an entire IGVH sequencing with IGHV-Leader primers (x axes) or partial sequencing with IGHV-FR1 primers (y axes). The percentage was obtained by dividing the number of mutations by the length (in nucleotides) of each region. One plot corresponds to one patient. Comparisons were performed in 64 patients by a Spearman's correlation test (correlation coefficient R2 = 0.97, P < 0.0001).

Mentions: To assess the reliability of the two methods for determining the mutational status, we compared data obtained with both primer sets (Fig.1) and showed a strong correlation (R2 = 0.97, P < 0.0001). As both protocols give the same results, we used the whole sequences obtained with IGHV-Leaders primers for further analyses.


Comprehensive characterization of immunoglobulin gene rearrangements in patients with chronic lymphocytic leukaemia.

René C, Prat N, Thuizat A, Broctawik M, Avinens O, Eliaou JF - J. Cell. Mol. Med. (2014)

Comparison of mutational rate according to the set of primers used. The IGVH mutation percentage was determined by either an entire IGVH sequencing with IGHV-Leader primers (x axes) or partial sequencing with IGHV-FR1 primers (y axes). The percentage was obtained by dividing the number of mutations by the length (in nucleotides) of each region. One plot corresponds to one patient. Comparisons were performed in 64 patients by a Spearman's correlation test (correlation coefficient R2 = 0.97, P < 0.0001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508139&req=5

fig01: Comparison of mutational rate according to the set of primers used. The IGVH mutation percentage was determined by either an entire IGVH sequencing with IGHV-Leader primers (x axes) or partial sequencing with IGHV-FR1 primers (y axes). The percentage was obtained by dividing the number of mutations by the length (in nucleotides) of each region. One plot corresponds to one patient. Comparisons were performed in 64 patients by a Spearman's correlation test (correlation coefficient R2 = 0.97, P < 0.0001).
Mentions: To assess the reliability of the two methods for determining the mutational status, we compared data obtained with both primer sets (Fig.1) and showed a strong correlation (R2 = 0.97, P < 0.0001). As both protocols give the same results, we used the whole sequences obtained with IGHV-Leaders primers for further analyses.

Bottom Line: Described biased usage of variable, diversity and joining genes between the mutated and unmutated groups was found in our population.Surprisingly, localization of activation-induced cytidine deaminase motifs onto the variable gene showed a preference for framework regions.The study of the characteristics at the age of diagnosis showed no difference in clinical outcome, but suggested a tendency of increased replacement and transition-over-transversion mutations and a longer third CDR length in older patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, CHRU de Montpellier, University Hospital Saint-Eloi, Montpellier, France; Faculté de Médecine, University of Montpellier 1, Montpellier, France.

Show MeSH
Related in: MedlinePlus