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Enucleation: a possible mechanism of cancer cell death.

Paunescu V, Bojin FM, Gavriliuc OI, Taculescu EA, Ianos R, Ordodi VL, Iman VF, Tatu CA - J. Cell. Mol. Med. (2014)

Bottom Line: There are few major morphologies of cell death that have been described so far: apoptosis (type I), cell death associated with autophagy (type II), necrosis (type III) and anchorage-dependent mechanisms-anoikis.Scanning and transmission electron microscopy revealed that tumour cells developed a network of intracytoplasmic stress fibres, which induce extrusion of nuclei, and enucleated cells die.Normal adult mesenchymal stem cells, used as control, did not exhibit the same behaviour.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Sciences, University of Medicine and Pharmacy "Victor Babes" Timisoara, Timisoara, Romania; Center for Transplant Immunology, Clinical Emergency County Hospital Timisoara, Timisoara, Romania.

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Scanning electron microscopy images of MSCs before and after the treatment with colloidal suspensions: (A) control (untreated) MSCs cells (3000×), (B) MSCs cells treated with MNPs suspension (5000×).
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fig02: Scanning electron microscopy images of MSCs before and after the treatment with colloidal suspensions: (A) control (untreated) MSCs cells (3000×), (B) MSCs cells treated with MNPs suspension (5000×).

Mentions: Morphological characteristics of untreated SK-BR-3 cells are depicted in Figure1A and C, showing the round shape, small diameter and cluster-like growth of this cell type in vitro, forming a network-like pattern of cellular elongations and contact points, with increased deposition of extracellular matrix within the resulted mesh. When left in contact with MNP for 48 hrs, SK-BR-3 tumour cells exhibited an unusual behaviour, extruding the nucleus, so that the cells were enucleated (Fig.1B and D). Based on quantification results obtained on 10 SEM fields/experiment, on average 90% of the tumour cells exhibited the loss of nucleus. To our knowledge, this is a novel biological phenomenon revelled for the first time. MCF-7 cells presented a very similar pattern of behaviour to SK-BR-3 cells, while the normal MSCs did not suffer the enucleation process (Fig.2A and B).


Enucleation: a possible mechanism of cancer cell death.

Paunescu V, Bojin FM, Gavriliuc OI, Taculescu EA, Ianos R, Ordodi VL, Iman VF, Tatu CA - J. Cell. Mol. Med. (2014)

Scanning electron microscopy images of MSCs before and after the treatment with colloidal suspensions: (A) control (untreated) MSCs cells (3000×), (B) MSCs cells treated with MNPs suspension (5000×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508136&req=5

fig02: Scanning electron microscopy images of MSCs before and after the treatment with colloidal suspensions: (A) control (untreated) MSCs cells (3000×), (B) MSCs cells treated with MNPs suspension (5000×).
Mentions: Morphological characteristics of untreated SK-BR-3 cells are depicted in Figure1A and C, showing the round shape, small diameter and cluster-like growth of this cell type in vitro, forming a network-like pattern of cellular elongations and contact points, with increased deposition of extracellular matrix within the resulted mesh. When left in contact with MNP for 48 hrs, SK-BR-3 tumour cells exhibited an unusual behaviour, extruding the nucleus, so that the cells were enucleated (Fig.1B and D). Based on quantification results obtained on 10 SEM fields/experiment, on average 90% of the tumour cells exhibited the loss of nucleus. To our knowledge, this is a novel biological phenomenon revelled for the first time. MCF-7 cells presented a very similar pattern of behaviour to SK-BR-3 cells, while the normal MSCs did not suffer the enucleation process (Fig.2A and B).

Bottom Line: There are few major morphologies of cell death that have been described so far: apoptosis (type I), cell death associated with autophagy (type II), necrosis (type III) and anchorage-dependent mechanisms-anoikis.Scanning and transmission electron microscopy revealed that tumour cells developed a network of intracytoplasmic stress fibres, which induce extrusion of nuclei, and enucleated cells die.Normal adult mesenchymal stem cells, used as control, did not exhibit the same behaviour.

View Article: PubMed Central - PubMed

Affiliation: Department of Functional Sciences, University of Medicine and Pharmacy "Victor Babes" Timisoara, Timisoara, Romania; Center for Transplant Immunology, Clinical Emergency County Hospital Timisoara, Timisoara, Romania.

Show MeSH
Related in: MedlinePlus