Limits...
Functional MRI during Hippocampal Deep Brain Stimulation in the Healthy Rat Brain.

Van Den Berge N, Vanhove C, Descamps B, Dauwe I, van Mierlo P, Vonck K, Keereman V, Raedt R, Boon P, Van Holen R - PLoS ONE (2015)

Bottom Line: The effects of DBS have been investigated primarily by electrophysiological and neurochemical studies, which lack the ability to investigate DBS-related responses on a whole-brain scale.Clusters were considered significant when p-values were <0.05 after correction for multiple comparisons.Our data indicate that real-time hippocampal DBS evokes a bilateral BOLD response in hippocampal and other mesolimbic structures, depending on the applied stimulation intensity.

View Article: PubMed Central - PubMed

Affiliation: Medical Image and Signal Processing Group, Ghent University-iMinds Medical IT department, Ghent, Belgium.

ABSTRACT
Deep Brain Stimulation (DBS) is a promising treatment for neurological and psychiatric disorders. The mechanism of action and the effects of electrical fields administered to the brain by means of an electrode remain to be elucidated. The effects of DBS have been investigated primarily by electrophysiological and neurochemical studies, which lack the ability to investigate DBS-related responses on a whole-brain scale. Visualization of whole-brain effects of DBS requires functional imaging techniques such as functional Magnetic Resonance Imaging (fMRI), which reflects changes in blood oxygen level dependent (BOLD) responses throughout the entire brain volume. In order to visualize BOLD responses induced by DBS, we have developed an MRI-compatible electrode and an acquisition protocol to perform DBS during BOLD fMRI. In this study, we investigate whether DBS during fMRI is valuable to study local and whole-brain effects of hippocampal DBS and to investigate the changes induced by different stimulation intensities. Seven rats were stereotactically implanted with a custom-made MRI-compatible DBS-electrode in the right hippocampus. High frequency Poisson distributed stimulation was applied using a block-design paradigm. Data were processed by means of Independent Component Analysis. Clusters were considered significant when p-values were <0.05 after correction for multiple comparisons. Our data indicate that real-time hippocampal DBS evokes a bilateral BOLD response in hippocampal and other mesolimbic structures, depending on the applied stimulation intensity. We conclude that simultaneous DBS and fMRI can be used to detect local and whole-brain responses to circuit activation with different stimulation intensities, making this technique potentially powerful for exploration of cerebral changes in response to DBS for both preclinical and clinical DBS.

No MeSH data available.


Related in: MedlinePlus

Illustration of electrode-artifact on MR-images.(a) structural MRI of axial slice at height of electrode. (b) functional MRI of axial slice through forebrain. (c) functional MRI of axial slice at height of electrode. (d) post-mortem structural MRI at height of the electrode path. The post-mortem MRI is used to verify the electrode track position. For all rats the electrode track was located in the right hippocampus.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4508110&req=5

pone.0133245.g003: Illustration of electrode-artifact on MR-images.(a) structural MRI of axial slice at height of electrode. (b) functional MRI of axial slice through forebrain. (c) functional MRI of axial slice at height of electrode. (d) post-mortem structural MRI at height of the electrode path. The post-mortem MRI is used to verify the electrode track position. For all rats the electrode track was located in the right hippocampus.

Mentions: The impact of the electrode artifact on the structural and functional MR image is illustrated in Fig 3a and 3b/3c, respectively. The electrode track obtained via a post-mortem MRI and after careful removal of the electrode, is shown in Fig 3d. We verified that the electrode track was in the right hippocampus for all subjects.


Functional MRI during Hippocampal Deep Brain Stimulation in the Healthy Rat Brain.

Van Den Berge N, Vanhove C, Descamps B, Dauwe I, van Mierlo P, Vonck K, Keereman V, Raedt R, Boon P, Van Holen R - PLoS ONE (2015)

Illustration of electrode-artifact on MR-images.(a) structural MRI of axial slice at height of electrode. (b) functional MRI of axial slice through forebrain. (c) functional MRI of axial slice at height of electrode. (d) post-mortem structural MRI at height of the electrode path. The post-mortem MRI is used to verify the electrode track position. For all rats the electrode track was located in the right hippocampus.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508110&req=5

pone.0133245.g003: Illustration of electrode-artifact on MR-images.(a) structural MRI of axial slice at height of electrode. (b) functional MRI of axial slice through forebrain. (c) functional MRI of axial slice at height of electrode. (d) post-mortem structural MRI at height of the electrode path. The post-mortem MRI is used to verify the electrode track position. For all rats the electrode track was located in the right hippocampus.
Mentions: The impact of the electrode artifact on the structural and functional MR image is illustrated in Fig 3a and 3b/3c, respectively. The electrode track obtained via a post-mortem MRI and after careful removal of the electrode, is shown in Fig 3d. We verified that the electrode track was in the right hippocampus for all subjects.

Bottom Line: The effects of DBS have been investigated primarily by electrophysiological and neurochemical studies, which lack the ability to investigate DBS-related responses on a whole-brain scale.Clusters were considered significant when p-values were <0.05 after correction for multiple comparisons.Our data indicate that real-time hippocampal DBS evokes a bilateral BOLD response in hippocampal and other mesolimbic structures, depending on the applied stimulation intensity.

View Article: PubMed Central - PubMed

Affiliation: Medical Image and Signal Processing Group, Ghent University-iMinds Medical IT department, Ghent, Belgium.

ABSTRACT
Deep Brain Stimulation (DBS) is a promising treatment for neurological and psychiatric disorders. The mechanism of action and the effects of electrical fields administered to the brain by means of an electrode remain to be elucidated. The effects of DBS have been investigated primarily by electrophysiological and neurochemical studies, which lack the ability to investigate DBS-related responses on a whole-brain scale. Visualization of whole-brain effects of DBS requires functional imaging techniques such as functional Magnetic Resonance Imaging (fMRI), which reflects changes in blood oxygen level dependent (BOLD) responses throughout the entire brain volume. In order to visualize BOLD responses induced by DBS, we have developed an MRI-compatible electrode and an acquisition protocol to perform DBS during BOLD fMRI. In this study, we investigate whether DBS during fMRI is valuable to study local and whole-brain effects of hippocampal DBS and to investigate the changes induced by different stimulation intensities. Seven rats were stereotactically implanted with a custom-made MRI-compatible DBS-electrode in the right hippocampus. High frequency Poisson distributed stimulation was applied using a block-design paradigm. Data were processed by means of Independent Component Analysis. Clusters were considered significant when p-values were <0.05 after correction for multiple comparisons. Our data indicate that real-time hippocampal DBS evokes a bilateral BOLD response in hippocampal and other mesolimbic structures, depending on the applied stimulation intensity. We conclude that simultaneous DBS and fMRI can be used to detect local and whole-brain responses to circuit activation with different stimulation intensities, making this technique potentially powerful for exploration of cerebral changes in response to DBS for both preclinical and clinical DBS.

No MeSH data available.


Related in: MedlinePlus