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Proteomic Characterisation of the Salt Gland-Enriched Tissues of the Mangrove Tree Species Avicennia officinalis.

Tan WK, Lim TK, Loh CS, Kumar P, Lin Q - PLoS ONE (2015)

Bottom Line: This paper reports for the first time the proteomic analysis of salt gland-enriched tissues of a mangrove tree species.Information obtained from this study paves the way for future proteomic research aiming at elucidating the molecular mechanism underlying secretion in plant salt glands.The data have been deposited to the ProteomeXchange with identifier PXD000771.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, Singapore, 117543; NUS Environmental Research Institute, National University of Singapore, 5A Engineering Drive 1, T-Lab, #02-01, Singapore, Singapore, 117411.

ABSTRACT
Plant salt glands are nature's desalination devices that harbour potentially useful information pertaining to salt and water transport during secretion. As part of the program toward deciphering secretion mechanisms in salt glands, we used shotgun proteomics to compare the protein profiles of salt gland-enriched (isolated epidermal peels) and salt gland-deprived (mesophyll) tissues of the mangrove species Avicennia officinalis. The purpose of the work is to identify proteins that are present in the salt gland-enriched tissues. An average of 2189 and 977 proteins were identified from the epidermal peel and mesophyll tissues, respectively. Among these, 2188 proteins were identified in salt gland-enriched tissues and a total of 1032 selected proteins were categorized by Gene Ontology (GO) analysis. This paper reports for the first time the proteomic analysis of salt gland-enriched tissues of a mangrove tree species. Candidate proteins that may play a role in the desalination process of the mangrove salt glands and their potential localization were identified. Information obtained from this study paves the way for future proteomic research aiming at elucidating the molecular mechanism underlying secretion in plant salt glands. The data have been deposited to the ProteomeXchange with identifier PXD000771.

No MeSH data available.


Related in: MedlinePlus

GO annotation of proteins identified in salt gland-enriched tissues of A. officinalis.A total of 1032 proteins were analysed. The proteins were classified based on GO for (A) biological process, cellular component and (B) molecular function. The major subcategories of molecular function (B) are shown in more detail on the left and right sides below the corresponding subcategories.
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pone.0133386.g003: GO annotation of proteins identified in salt gland-enriched tissues of A. officinalis.A total of 1032 proteins were analysed. The proteins were classified based on GO for (A) biological process, cellular component and (B) molecular function. The major subcategories of molecular function (B) are shown in more detail on the left and right sides below the corresponding subcategories.

Mentions: To better understand the functions of proteins identified in salt gland-enriched tissues, proteins that are present in at least two of the biological replicates were selected from the list of 2188 proteins for GO analysis [22]. A total of 1032 selected proteins (see S8 Table) were analysed and information were retrieved from the UniProt Knowledgebase website (http://www.uniprot.org/help/uniprotkb). The proteins were annotated based on three organizing principles of GO (Fig 3). They were characterised by their function in diverse biological processes, with 11 sub-categories identified (Fig 3A). Majority of these proteins were predicted to participate in metabolic (24%), cellular (22%) or single-organism (13%) processes or were responding to stimulus (9%), if not involved in localization (12%) (Fig 3A).


Proteomic Characterisation of the Salt Gland-Enriched Tissues of the Mangrove Tree Species Avicennia officinalis.

Tan WK, Lim TK, Loh CS, Kumar P, Lin Q - PLoS ONE (2015)

GO annotation of proteins identified in salt gland-enriched tissues of A. officinalis.A total of 1032 proteins were analysed. The proteins were classified based on GO for (A) biological process, cellular component and (B) molecular function. The major subcategories of molecular function (B) are shown in more detail on the left and right sides below the corresponding subcategories.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4508094&req=5

pone.0133386.g003: GO annotation of proteins identified in salt gland-enriched tissues of A. officinalis.A total of 1032 proteins were analysed. The proteins were classified based on GO for (A) biological process, cellular component and (B) molecular function. The major subcategories of molecular function (B) are shown in more detail on the left and right sides below the corresponding subcategories.
Mentions: To better understand the functions of proteins identified in salt gland-enriched tissues, proteins that are present in at least two of the biological replicates were selected from the list of 2188 proteins for GO analysis [22]. A total of 1032 selected proteins (see S8 Table) were analysed and information were retrieved from the UniProt Knowledgebase website (http://www.uniprot.org/help/uniprotkb). The proteins were annotated based on three organizing principles of GO (Fig 3). They were characterised by their function in diverse biological processes, with 11 sub-categories identified (Fig 3A). Majority of these proteins were predicted to participate in metabolic (24%), cellular (22%) or single-organism (13%) processes or were responding to stimulus (9%), if not involved in localization (12%) (Fig 3A).

Bottom Line: This paper reports for the first time the proteomic analysis of salt gland-enriched tissues of a mangrove tree species.Information obtained from this study paves the way for future proteomic research aiming at elucidating the molecular mechanism underlying secretion in plant salt glands.The data have been deposited to the ProteomeXchange with identifier PXD000771.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore, Singapore, 117543; NUS Environmental Research Institute, National University of Singapore, 5A Engineering Drive 1, T-Lab, #02-01, Singapore, Singapore, 117411.

ABSTRACT
Plant salt glands are nature's desalination devices that harbour potentially useful information pertaining to salt and water transport during secretion. As part of the program toward deciphering secretion mechanisms in salt glands, we used shotgun proteomics to compare the protein profiles of salt gland-enriched (isolated epidermal peels) and salt gland-deprived (mesophyll) tissues of the mangrove species Avicennia officinalis. The purpose of the work is to identify proteins that are present in the salt gland-enriched tissues. An average of 2189 and 977 proteins were identified from the epidermal peel and mesophyll tissues, respectively. Among these, 2188 proteins were identified in salt gland-enriched tissues and a total of 1032 selected proteins were categorized by Gene Ontology (GO) analysis. This paper reports for the first time the proteomic analysis of salt gland-enriched tissues of a mangrove tree species. Candidate proteins that may play a role in the desalination process of the mangrove salt glands and their potential localization were identified. Information obtained from this study paves the way for future proteomic research aiming at elucidating the molecular mechanism underlying secretion in plant salt glands. The data have been deposited to the ProteomeXchange with identifier PXD000771.

No MeSH data available.


Related in: MedlinePlus