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The Crosstalk between Osteoclasts and Osteoblasts Is Dependent upon the Composition and Structure of Biphasic Calcium Phosphates.

Shiwaku Y, Neff L, Nagano K, Takeyama K, de Bruijn J, Dard M, Gori F, Baron R - PLoS ONE (2015)

Bottom Line: We found that BCPs with low percentage of HA favored the expression of positive coupling factors, including sphingosine-kinase 1 (SPHK1) and collagen triple helix repeat containing 1 (Cthrc1).In turn, the increase of these secreted coupling factors promotes OB differentiation and function.All together our studies suggest that the chemical composition of biomaterials affects not only the differentiation and activity of OCs but also their potential to locally regulate bone formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA, United States of America.

ABSTRACT
Biphasic calcium phosphates (BCPs), consisting of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), exhibit good biocompatibility and osteoconductivity, maintaining a balance between resorption of the biomaterial and formation of new bone. We tested whether the chemical composition and/or the microstructure of BCPs affect osteoclasts (OCs) differentiation and/or their ability to crosstalk with osteoblasts (OBs). To this aim, OCs were cultured on BCPs with HA content of 5, 20 or 60% and their differentiation and activity were assessed. We found that OC differentiation is partially impaired by increased HA content, but not by the presence of micropores within BCP scaffolds, as indicated by TRAP staining and gene profile expression. We then investigated whether the biomaterial-induced changes in OC differentiation also affect their ability to crosstalk with OBs and regulate OB function. We found that BCPs with low percentage of HA favored the expression of positive coupling factors, including sphingosine-kinase 1 (SPHK1) and collagen triple helix repeat containing 1 (Cthrc1). In turn, the increase of these secreted coupling factors promotes OB differentiation and function. All together our studies suggest that the chemical composition of biomaterials affects not only the differentiation and activity of OCs but also their potential to locally regulate bone formation.

No MeSH data available.


Related in: MedlinePlus

XRD patterns of BCP disks.(A) 5HA porous, (B) 5HA dense, (C) 20HA dense and (D) 60HA dense.
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pone.0132903.g001: XRD patterns of BCP disks.(A) 5HA porous, (B) 5HA dense, (C) 20HA dense and (D) 60HA dense.

Mentions: The chemical composition of BCPs was characterized by XRD (Fig 1). XRD patterns of 5HA dense and 5HA porous showed highly crystallized phases and possessed the typical reflection of β-TCP [27,28]. 60HA dense and 20HA dense displayed the peaks of both β-TCP and HA. Compared to 20HA dense, 60HA dense possesses more apatitic peaks.


The Crosstalk between Osteoclasts and Osteoblasts Is Dependent upon the Composition and Structure of Biphasic Calcium Phosphates.

Shiwaku Y, Neff L, Nagano K, Takeyama K, de Bruijn J, Dard M, Gori F, Baron R - PLoS ONE (2015)

XRD patterns of BCP disks.(A) 5HA porous, (B) 5HA dense, (C) 20HA dense and (D) 60HA dense.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507990&req=5

pone.0132903.g001: XRD patterns of BCP disks.(A) 5HA porous, (B) 5HA dense, (C) 20HA dense and (D) 60HA dense.
Mentions: The chemical composition of BCPs was characterized by XRD (Fig 1). XRD patterns of 5HA dense and 5HA porous showed highly crystallized phases and possessed the typical reflection of β-TCP [27,28]. 60HA dense and 20HA dense displayed the peaks of both β-TCP and HA. Compared to 20HA dense, 60HA dense possesses more apatitic peaks.

Bottom Line: We found that BCPs with low percentage of HA favored the expression of positive coupling factors, including sphingosine-kinase 1 (SPHK1) and collagen triple helix repeat containing 1 (Cthrc1).In turn, the increase of these secreted coupling factors promotes OB differentiation and function.All together our studies suggest that the chemical composition of biomaterials affects not only the differentiation and activity of OCs but also their potential to locally regulate bone formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Oral Medicine, Infection and Immunity, Harvard School of Dental Medicine, Boston, MA, United States of America.

ABSTRACT
Biphasic calcium phosphates (BCPs), consisting of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), exhibit good biocompatibility and osteoconductivity, maintaining a balance between resorption of the biomaterial and formation of new bone. We tested whether the chemical composition and/or the microstructure of BCPs affect osteoclasts (OCs) differentiation and/or their ability to crosstalk with osteoblasts (OBs). To this aim, OCs were cultured on BCPs with HA content of 5, 20 or 60% and their differentiation and activity were assessed. We found that OC differentiation is partially impaired by increased HA content, but not by the presence of micropores within BCP scaffolds, as indicated by TRAP staining and gene profile expression. We then investigated whether the biomaterial-induced changes in OC differentiation also affect their ability to crosstalk with OBs and regulate OB function. We found that BCPs with low percentage of HA favored the expression of positive coupling factors, including sphingosine-kinase 1 (SPHK1) and collagen triple helix repeat containing 1 (Cthrc1). In turn, the increase of these secreted coupling factors promotes OB differentiation and function. All together our studies suggest that the chemical composition of biomaterials affects not only the differentiation and activity of OCs but also their potential to locally regulate bone formation.

No MeSH data available.


Related in: MedlinePlus