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Comparative Transcriptome Analysis Reveals Sex-Biased Gene Expression in Juvenile Chinese Mitten Crab Eriocheir sinensis.

Liu Y, Hui M, Cui Z, Luo D, Song C, Li Y, Liu L - PLoS ONE (2015)

Bottom Line: Sex-biased genes are considered to account for most of phenotypic differences between males and females.Most of DEGs were involved in processes such as metabolism and immunity, and not associated with obvious sexual function.In addition, two reproduction-related genes, vitellogenin and insulin-like androgenic gland factor, were identified to express in both sexes but with significantly higher level in males.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

ABSTRACT
Sex-biased genes are considered to account for most of phenotypic differences between males and females. In order to explore the sex-biased gene expression in crab, we performed the whole-body transcriptome analysis in male and female juveniles of the Chinese mitten crab Eriocheir sinensis using next-generation sequencing technology. Of the 23,349 annotated unigenes, 148 were identified as sex-related genes. A total of 29 candidate genes involved in primary sex determination pathways were detected, indicating the sex determination cascade of the mitten crab might be more complex than previously supposed. Differential expression analysis showed 448 differentially expressed genes (DEGs) between the two transcriptomes. Most of DEGs were involved in processes such as metabolism and immunity, and not associated with obvious sexual function. The pathway predominantly enriched for DEGs were related to lysosome, which might reflect the differences in metabolism between males and females. Of the immune DGEs, 18 up-regulated genes in females were humoral immune factors, and eight up-regulated genes in males were pattern recognition receptors, suggesting sex differences of immune defense might exist in the mitten crab. In addition, two reproduction-related genes, vitellogenin and insulin-like androgenic gland factor, were identified to express in both sexes but with significantly higher level in males. Our research provides the first whole-body RNA sequencing of sex-specific transcriptomes for juvenile E. sinensis and will facilitate further studies on molecular mechanisms of crab sexual dimorphism.

No MeSH data available.


Related in: MedlinePlus

Expression pattern of genes involved in lysosomal pathway.The pathway is based on a KEGG pathway analysis. The up-regulated genes in male and female are labeled by green and red, respectively, and the purple color represents genes with no expression differences between female and male transcriptomes of Eriocheir sinensis.
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pone.0133068.g003: Expression pattern of genes involved in lysosomal pathway.The pathway is based on a KEGG pathway analysis. The up-regulated genes in male and female are labeled by green and red, respectively, and the purple color represents genes with no expression differences between female and male transcriptomes of Eriocheir sinensis.

Mentions: KEGG analysis revealed that 139 DEGs with KO terms were involved in 29 predicated biological pathways (S5 Fig). The most abundant pathways included ‘carbohydrate metabolism’ (36 DGEs), ‘glycan biosynthesis and metabolism’ (24 DGEs), ‘transport and catabolism’ (24 DGEs) and ‘lipid metabolism’ (16 DGEs). The significantly distinct categories were ‘carbohydrate metabolism’, ‘glycan biosynthesis and metabolism’, ‘metabolism of cofactors and vitamins’, ‘transport and catabolism’, ‘digestive system, ‘lipid metabolism’, ‘xenobiotics biodegradation and metabolism’ and ‘amino acid metabolism’ (p < 0.05, S5 Fig). Except for metabolic pathway, the pathway predominantly enriched for DEGs were related to lysosome (ko04142) of ‘transport and catabolism’ (Fig 3). There were 19 DEGs with ten KO terms in lysosomal pathway, including 18 up-regulated in males and one up-regulated in females. In addition, one important pathway identified as enriched for the up-regulated genes in males were associated with sphingolipid metabolism (ko00600) of ‘lipid metabolism’ (S6 Fig).


Comparative Transcriptome Analysis Reveals Sex-Biased Gene Expression in Juvenile Chinese Mitten Crab Eriocheir sinensis.

Liu Y, Hui M, Cui Z, Luo D, Song C, Li Y, Liu L - PLoS ONE (2015)

Expression pattern of genes involved in lysosomal pathway.The pathway is based on a KEGG pathway analysis. The up-regulated genes in male and female are labeled by green and red, respectively, and the purple color represents genes with no expression differences between female and male transcriptomes of Eriocheir sinensis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507985&req=5

pone.0133068.g003: Expression pattern of genes involved in lysosomal pathway.The pathway is based on a KEGG pathway analysis. The up-regulated genes in male and female are labeled by green and red, respectively, and the purple color represents genes with no expression differences between female and male transcriptomes of Eriocheir sinensis.
Mentions: KEGG analysis revealed that 139 DEGs with KO terms were involved in 29 predicated biological pathways (S5 Fig). The most abundant pathways included ‘carbohydrate metabolism’ (36 DGEs), ‘glycan biosynthesis and metabolism’ (24 DGEs), ‘transport and catabolism’ (24 DGEs) and ‘lipid metabolism’ (16 DGEs). The significantly distinct categories were ‘carbohydrate metabolism’, ‘glycan biosynthesis and metabolism’, ‘metabolism of cofactors and vitamins’, ‘transport and catabolism’, ‘digestive system, ‘lipid metabolism’, ‘xenobiotics biodegradation and metabolism’ and ‘amino acid metabolism’ (p < 0.05, S5 Fig). Except for metabolic pathway, the pathway predominantly enriched for DEGs were related to lysosome (ko04142) of ‘transport and catabolism’ (Fig 3). There were 19 DEGs with ten KO terms in lysosomal pathway, including 18 up-regulated in males and one up-regulated in females. In addition, one important pathway identified as enriched for the up-regulated genes in males were associated with sphingolipid metabolism (ko00600) of ‘lipid metabolism’ (S6 Fig).

Bottom Line: Sex-biased genes are considered to account for most of phenotypic differences between males and females.Most of DEGs were involved in processes such as metabolism and immunity, and not associated with obvious sexual function.In addition, two reproduction-related genes, vitellogenin and insulin-like androgenic gland factor, were identified to express in both sexes but with significantly higher level in males.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

ABSTRACT
Sex-biased genes are considered to account for most of phenotypic differences between males and females. In order to explore the sex-biased gene expression in crab, we performed the whole-body transcriptome analysis in male and female juveniles of the Chinese mitten crab Eriocheir sinensis using next-generation sequencing technology. Of the 23,349 annotated unigenes, 148 were identified as sex-related genes. A total of 29 candidate genes involved in primary sex determination pathways were detected, indicating the sex determination cascade of the mitten crab might be more complex than previously supposed. Differential expression analysis showed 448 differentially expressed genes (DEGs) between the two transcriptomes. Most of DEGs were involved in processes such as metabolism and immunity, and not associated with obvious sexual function. The pathway predominantly enriched for DEGs were related to lysosome, which might reflect the differences in metabolism between males and females. Of the immune DGEs, 18 up-regulated genes in females were humoral immune factors, and eight up-regulated genes in males were pattern recognition receptors, suggesting sex differences of immune defense might exist in the mitten crab. In addition, two reproduction-related genes, vitellogenin and insulin-like androgenic gland factor, were identified to express in both sexes but with significantly higher level in males. Our research provides the first whole-body RNA sequencing of sex-specific transcriptomes for juvenile E. sinensis and will facilitate further studies on molecular mechanisms of crab sexual dimorphism.

No MeSH data available.


Related in: MedlinePlus