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Genistein-induced mir-23b expression inhibits the growth of breast cancer cells.

Avci CB, Susluer SY, Caglar HO, Balci T, Aygunes D, Dodurga Y, Gunduz C - Contemp Oncol (Pozn) (2014)

Bottom Line: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment.Expressions of miRNAs were quantified using Real-Time Online RT-PCR.The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.

ABSTRACT

Aim of the study: Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.

Material and methods: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.

Results: The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.

Conclusions: Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.

No MeSH data available.


Related in: MedlinePlus

miRNA expression profiles after treatment. For the genistein group, miR-23b was up-regulated 56.69 fold after treatment. miRNA expression visualization about log2 (Fold Change) associated with genistein, compared with control
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Figure 0002: miRNA expression profiles after treatment. For the genistein group, miR-23b was up-regulated 56.69 fold after treatment. miRNA expression visualization about log2 (Fold Change) associated with genistein, compared with control

Mentions: Alterations in the expressions of miRNAs were compared with genistein untreated MCF-7 cells. miRNA expression was detected 48 hours after genistein treatment. SNORD44, SNORD47, SNORD48, and U6 genes were used for housekeeping miRNAs as the endogenous normalisation factor to define miRNA expression profiles of 88 miRNAs. miR-23b was found to be up-regulated 56.69 fold in the treatment of genistein compared to the control group of genistein untreated cells (Fig. 2).


Genistein-induced mir-23b expression inhibits the growth of breast cancer cells.

Avci CB, Susluer SY, Caglar HO, Balci T, Aygunes D, Dodurga Y, Gunduz C - Contemp Oncol (Pozn) (2014)

miRNA expression profiles after treatment. For the genistein group, miR-23b was up-regulated 56.69 fold after treatment. miRNA expression visualization about log2 (Fold Change) associated with genistein, compared with control
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507883&req=5

Figure 0002: miRNA expression profiles after treatment. For the genistein group, miR-23b was up-regulated 56.69 fold after treatment. miRNA expression visualization about log2 (Fold Change) associated with genistein, compared with control
Mentions: Alterations in the expressions of miRNAs were compared with genistein untreated MCF-7 cells. miRNA expression was detected 48 hours after genistein treatment. SNORD44, SNORD47, SNORD48, and U6 genes were used for housekeeping miRNAs as the endogenous normalisation factor to define miRNA expression profiles of 88 miRNAs. miR-23b was found to be up-regulated 56.69 fold in the treatment of genistein compared to the control group of genistein untreated cells (Fig. 2).

Bottom Line: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment.Expressions of miRNAs were quantified using Real-Time Online RT-PCR.The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.

ABSTRACT

Aim of the study: Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.

Material and methods: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.

Results: The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.

Conclusions: Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.

No MeSH data available.


Related in: MedlinePlus