Limits...
Genistein-induced mir-23b expression inhibits the growth of breast cancer cells.

Avci CB, Susluer SY, Caglar HO, Balci T, Aygunes D, Dodurga Y, Gunduz C - Contemp Oncol (Pozn) (2014)

Bottom Line: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment.Expressions of miRNAs were quantified using Real-Time Online RT-PCR.The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.

ABSTRACT

Aim of the study: Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.

Material and methods: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.

Results: The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.

Conclusions: Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.

No MeSH data available.


Related in: MedlinePlus

Dose-dependent cytotoxicity of genistein. MCF-7 cells were treated with various concentrations of genistein. The studied concentrations of genistein were 75 μM, 100 μM, 125 μM, 150 μM, 175 μM, and 200 μM. The IC50 dose of genistein was 175 μM
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4507883&req=5

Figure 0001: Dose-dependent cytotoxicity of genistein. MCF-7 cells were treated with various concentrations of genistein. The studied concentrations of genistein were 75 μM, 100 μM, 125 μM, 150 μM, 175 μM, and 200 μM. The IC50 dose of genistein was 175 μM

Mentions: Cells were incubated at a density of 2 × 105 cells/ml of medium using 96-well plates for 24, 48, and 72 hours. Studied concentrations of genistein were 75 µM, 100 µM, 125 µM, 150 µM, 175 µM, and 200 µM (Fig. 1). Untreated MCF-7 cells were considered as a control group. The IC50 dose of genistein was 175 µM and the cytotoxic effect of genistein was detected after 48 hours.


Genistein-induced mir-23b expression inhibits the growth of breast cancer cells.

Avci CB, Susluer SY, Caglar HO, Balci T, Aygunes D, Dodurga Y, Gunduz C - Contemp Oncol (Pozn) (2014)

Dose-dependent cytotoxicity of genistein. MCF-7 cells were treated with various concentrations of genistein. The studied concentrations of genistein were 75 μM, 100 μM, 125 μM, 150 μM, 175 μM, and 200 μM. The IC50 dose of genistein was 175 μM
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507883&req=5

Figure 0001: Dose-dependent cytotoxicity of genistein. MCF-7 cells were treated with various concentrations of genistein. The studied concentrations of genistein were 75 μM, 100 μM, 125 μM, 150 μM, 175 μM, and 200 μM. The IC50 dose of genistein was 175 μM
Mentions: Cells were incubated at a density of 2 × 105 cells/ml of medium using 96-well plates for 24, 48, and 72 hours. Studied concentrations of genistein were 75 µM, 100 µM, 125 µM, 150 µM, 175 µM, and 200 µM (Fig. 1). Untreated MCF-7 cells were considered as a control group. The IC50 dose of genistein was 175 µM and the cytotoxic effect of genistein was detected after 48 hours.

Bottom Line: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment.Expressions of miRNAs were quantified using Real-Time Online RT-PCR.The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biology, Medical Faculty, Ege University, Bornova, Izmir, Turkey.

ABSTRACT

Aim of the study: Genistein, an isoflavonoid, plays roles in the inhibition of protein tyrosine kinase phosphorylation, induction of apoptosis, and cell differentiation in breast cancer. This study aims to induce cellular stress by exposing genistein to determine alterations of miRNA expression profiles in MCF-7 cells.

Material and methods: XTT assay and trypan blue dye exclusion assays were performed to examine the cytotoxic effects of genistein treatment. Expressions of miRNAs were quantified using Real-Time Online RT-PCR.

Results: The IC50 dose of genistein was 175 μM in MCF-7 cell, line and the cytotoxic effect of genistein was detected after 48 hours. miR-23b was found to be up-regulated 56.69 fold following the treatment of genistein. It was found that miR-23b was upregulated for MCF-7 breast cancer cells after genistein treatment.

Conclusions: Up-regulated ex-expression of miR-23b might be a putative biomarker for use in the therapy of breast cancer patients. miR-23b up-regulation might be important in terms of response to genistein.

No MeSH data available.


Related in: MedlinePlus