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The Daily Profiles of Circulating AMH and INSL3 in Men are Distinct from the Other Testicular Hormones, Inhibin B and Testosterone.

Chong YH, Pankhurst MW, McLennan IS - PLoS ONE (2015)

Bottom Line: The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B.In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type.The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand; Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.

ABSTRACT
The testes secrete four hormones (anti-Müllerian hormone, insulin-like peptide 3, Inhibin B and testosterone) from two endocrine cell types. It is unknown whether anti-Müllerian hormone and insulin-like peptide 3 levels have a diurnal variation, and if so, whether they covary during the day with testosterone and InhB. Sera were obtained from 13 men at 00:00, 06:00, 09:00, 12:00, 14:00, 17:00 and 19:00 hours and the levels of their testicular hormones measured by ELISA. A second cohort of 20 men was similarly examined with blood drawn at 19:00 and the following 06:00. Anti-Müllerian hormone levels exhibited a subtle diurnal pattern with a 19:00 peak that was 4.9% higher on average than the 06:00 nadir (p = 0.004). The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B. Insulin-like peptide 3 had no diurnal pattern, with only minor sporadic variation between time points being observed in some men. In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type. Consequently, the balance of the hormones being released by the testes has complex variation during the day. The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

No MeSH data available.


Related in: MedlinePlus

Changes in serum testicular hormonal concentration between 19:00 and 06:00 (Study 2).(A) AMH, (B) INSL and (C) InhB and (D) testosterone levels were analysed in 20 men. AMH, on average declined by 4.9 ± 1.8%, (p = 0.004, paired Student t-test), whereas the levels of InhB and testosterone increased on average by 28 ± 4% (p<0.0005) and 41% (p = 0.011) respectively. There was no significant change in the levels of INSL3, on average (p = 0.78). The mean levels of the hormones at 19:00 and 06:00 were: AMH (57.9 ± 5.7, 55.0 ± 5.3 pM); InhB (147 ± 13, 189 ± 17 pg/ml); INSL3 (2.28 ± 0.20, 2.21 ± 0.24 ng/ml); testosterone (16.0 ± 1.9, 24.5 ± 2.0 nM).
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pone.0133637.g003: Changes in serum testicular hormonal concentration between 19:00 and 06:00 (Study 2).(A) AMH, (B) INSL and (C) InhB and (D) testosterone levels were analysed in 20 men. AMH, on average declined by 4.9 ± 1.8%, (p = 0.004, paired Student t-test), whereas the levels of InhB and testosterone increased on average by 28 ± 4% (p<0.0005) and 41% (p = 0.011) respectively. There was no significant change in the levels of INSL3, on average (p = 0.78). The mean levels of the hormones at 19:00 and 06:00 were: AMH (57.9 ± 5.7, 55.0 ± 5.3 pM); InhB (147 ± 13, 189 ± 17 pg/ml); INSL3 (2.28 ± 0.20, 2.21 ± 0.24 ng/ml); testosterone (16.0 ± 1.9, 24.5 ± 2.0 nM).

Mentions: There was no statistical variation in AMH across the day in study 1 (F(6,12) = 0.69, p = 0.48), indicating the absence of a consistent and overt diurnal profile. However, AMH levels were moderately lower in the nocturnal phase, with a nadir at 06:00, and a subsequent rise in the hours after waking (Figs 1A and 2A). The veracity of this subtle variation was tested in a larger cohort of 20 men, with a target comparison between their 06:00 and 19:00 values. This confirmed that the 06:00 levels were slightly lower on average (4.9%, p = 0.004, paired Student t-test), with the inter-person variation encompassing a range between -7.9% and 18.1% (Fig 3).


The Daily Profiles of Circulating AMH and INSL3 in Men are Distinct from the Other Testicular Hormones, Inhibin B and Testosterone.

Chong YH, Pankhurst MW, McLennan IS - PLoS ONE (2015)

Changes in serum testicular hormonal concentration between 19:00 and 06:00 (Study 2).(A) AMH, (B) INSL and (C) InhB and (D) testosterone levels were analysed in 20 men. AMH, on average declined by 4.9 ± 1.8%, (p = 0.004, paired Student t-test), whereas the levels of InhB and testosterone increased on average by 28 ± 4% (p<0.0005) and 41% (p = 0.011) respectively. There was no significant change in the levels of INSL3, on average (p = 0.78). The mean levels of the hormones at 19:00 and 06:00 were: AMH (57.9 ± 5.7, 55.0 ± 5.3 pM); InhB (147 ± 13, 189 ± 17 pg/ml); INSL3 (2.28 ± 0.20, 2.21 ± 0.24 ng/ml); testosterone (16.0 ± 1.9, 24.5 ± 2.0 nM).
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4507845&req=5

pone.0133637.g003: Changes in serum testicular hormonal concentration between 19:00 and 06:00 (Study 2).(A) AMH, (B) INSL and (C) InhB and (D) testosterone levels were analysed in 20 men. AMH, on average declined by 4.9 ± 1.8%, (p = 0.004, paired Student t-test), whereas the levels of InhB and testosterone increased on average by 28 ± 4% (p<0.0005) and 41% (p = 0.011) respectively. There was no significant change in the levels of INSL3, on average (p = 0.78). The mean levels of the hormones at 19:00 and 06:00 were: AMH (57.9 ± 5.7, 55.0 ± 5.3 pM); InhB (147 ± 13, 189 ± 17 pg/ml); INSL3 (2.28 ± 0.20, 2.21 ± 0.24 ng/ml); testosterone (16.0 ± 1.9, 24.5 ± 2.0 nM).
Mentions: There was no statistical variation in AMH across the day in study 1 (F(6,12) = 0.69, p = 0.48), indicating the absence of a consistent and overt diurnal profile. However, AMH levels were moderately lower in the nocturnal phase, with a nadir at 06:00, and a subsequent rise in the hours after waking (Figs 1A and 2A). The veracity of this subtle variation was tested in a larger cohort of 20 men, with a target comparison between their 06:00 and 19:00 values. This confirmed that the 06:00 levels were slightly lower on average (4.9%, p = 0.004, paired Student t-test), with the inter-person variation encompassing a range between -7.9% and 18.1% (Fig 3).

Bottom Line: The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B.In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type.The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand; Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.

ABSTRACT
The testes secrete four hormones (anti-Müllerian hormone, insulin-like peptide 3, Inhibin B and testosterone) from two endocrine cell types. It is unknown whether anti-Müllerian hormone and insulin-like peptide 3 levels have a diurnal variation, and if so, whether they covary during the day with testosterone and InhB. Sera were obtained from 13 men at 00:00, 06:00, 09:00, 12:00, 14:00, 17:00 and 19:00 hours and the levels of their testicular hormones measured by ELISA. A second cohort of 20 men was similarly examined with blood drawn at 19:00 and the following 06:00. Anti-Müllerian hormone levels exhibited a subtle diurnal pattern with a 19:00 peak that was 4.9% higher on average than the 06:00 nadir (p = 0.004). The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B. Insulin-like peptide 3 had no diurnal pattern, with only minor sporadic variation between time points being observed in some men. In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type. Consequently, the balance of the hormones being released by the testes has complex variation during the day. The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

No MeSH data available.


Related in: MedlinePlus