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The Daily Profiles of Circulating AMH and INSL3 in Men are Distinct from the Other Testicular Hormones, Inhibin B and Testosterone.

Chong YH, Pankhurst MW, McLennan IS - PLoS ONE (2015)

Bottom Line: The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B.In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type.The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand; Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.

ABSTRACT
The testes secrete four hormones (anti-Müllerian hormone, insulin-like peptide 3, Inhibin B and testosterone) from two endocrine cell types. It is unknown whether anti-Müllerian hormone and insulin-like peptide 3 levels have a diurnal variation, and if so, whether they covary during the day with testosterone and InhB. Sera were obtained from 13 men at 00:00, 06:00, 09:00, 12:00, 14:00, 17:00 and 19:00 hours and the levels of their testicular hormones measured by ELISA. A second cohort of 20 men was similarly examined with blood drawn at 19:00 and the following 06:00. Anti-Müllerian hormone levels exhibited a subtle diurnal pattern with a 19:00 peak that was 4.9% higher on average than the 06:00 nadir (p = 0.004). The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B. Insulin-like peptide 3 had no diurnal pattern, with only minor sporadic variation between time points being observed in some men. In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type. Consequently, the balance of the hormones being released by the testes has complex variation during the day. The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

No MeSH data available.


Related in: MedlinePlus

Study 1: diurnal pattern of hormonal expression.Each individual’s hormone levels were normalised to that individual’s mean across the entire sample period. (A) Sertoli cell hormones: InB (blue circles) and AMH (green triangles); (B) Leydig cell hormones: testosterone (red circles) and INSL3 (orange diamonds); (C) Gonadotropins: LH (brown diamonds) and FSH (pink circle) and (D) Circadian marker: Cortisol. The single 0:00 data point is plotted at both 0:00 and 24:00 with the dashed line used to indicate this extrapolation. The data are the mean ± the standard error of 13 men.
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pone.0133637.g001: Study 1: diurnal pattern of hormonal expression.Each individual’s hormone levels were normalised to that individual’s mean across the entire sample period. (A) Sertoli cell hormones: InB (blue circles) and AMH (green triangles); (B) Leydig cell hormones: testosterone (red circles) and INSL3 (orange diamonds); (C) Gonadotropins: LH (brown diamonds) and FSH (pink circle) and (D) Circadian marker: Cortisol. The single 0:00 data point is plotted at both 0:00 and 24:00 with the dashed line used to indicate this extrapolation. The data are the mean ± the standard error of 13 men.

Mentions: All men in Study 1 exhibited a normal daily pattern of cortisol (Fig 1D) [22], verifying that their endogenous circadian rhythm was intact. LH had a significant diurnal variation as expected [23, 24], with higher values in the nocturnal and early morning periods, and subsequently trending towards an evening nadir (F(6,12) = 3.95, p = 0.032) (Fig 1C). FSH, in contrast, did not vary significantly between time points (F(6,12) = 1.05, p = 0.36) (Fig 1C). Similar results were obtained for LH and FSH in a validation experiment (Study 2) comparing 6:00 and 19:00 values in 20 men (LH 6:00 4.7 ± 0.5 IU vs 19:00 3.7 ± 0.3 IU, p = 0.032 paired student t test; FSH 6am 4.1 ± 0.5 IU vs 7 pm 4.0 ± 0.4 IU, not significantly different).


The Daily Profiles of Circulating AMH and INSL3 in Men are Distinct from the Other Testicular Hormones, Inhibin B and Testosterone.

Chong YH, Pankhurst MW, McLennan IS - PLoS ONE (2015)

Study 1: diurnal pattern of hormonal expression.Each individual’s hormone levels were normalised to that individual’s mean across the entire sample period. (A) Sertoli cell hormones: InB (blue circles) and AMH (green triangles); (B) Leydig cell hormones: testosterone (red circles) and INSL3 (orange diamonds); (C) Gonadotropins: LH (brown diamonds) and FSH (pink circle) and (D) Circadian marker: Cortisol. The single 0:00 data point is plotted at both 0:00 and 24:00 with the dashed line used to indicate this extrapolation. The data are the mean ± the standard error of 13 men.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4507845&req=5

pone.0133637.g001: Study 1: diurnal pattern of hormonal expression.Each individual’s hormone levels were normalised to that individual’s mean across the entire sample period. (A) Sertoli cell hormones: InB (blue circles) and AMH (green triangles); (B) Leydig cell hormones: testosterone (red circles) and INSL3 (orange diamonds); (C) Gonadotropins: LH (brown diamonds) and FSH (pink circle) and (D) Circadian marker: Cortisol. The single 0:00 data point is plotted at both 0:00 and 24:00 with the dashed line used to indicate this extrapolation. The data are the mean ± the standard error of 13 men.
Mentions: All men in Study 1 exhibited a normal daily pattern of cortisol (Fig 1D) [22], verifying that their endogenous circadian rhythm was intact. LH had a significant diurnal variation as expected [23, 24], with higher values in the nocturnal and early morning periods, and subsequently trending towards an evening nadir (F(6,12) = 3.95, p = 0.032) (Fig 1C). FSH, in contrast, did not vary significantly between time points (F(6,12) = 1.05, p = 0.36) (Fig 1C). Similar results were obtained for LH and FSH in a validation experiment (Study 2) comparing 6:00 and 19:00 values in 20 men (LH 6:00 4.7 ± 0.5 IU vs 19:00 3.7 ± 0.3 IU, p = 0.032 paired student t test; FSH 6am 4.1 ± 0.5 IU vs 7 pm 4.0 ± 0.4 IU, not significantly different).

Bottom Line: The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B.In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type.The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand; Department of Medicine, Dunedin School of Medicine, University of Otago, Dunedin, New Zealand.

ABSTRACT
The testes secrete four hormones (anti-Müllerian hormone, insulin-like peptide 3, Inhibin B and testosterone) from two endocrine cell types. It is unknown whether anti-Müllerian hormone and insulin-like peptide 3 levels have a diurnal variation, and if so, whether they covary during the day with testosterone and InhB. Sera were obtained from 13 men at 00:00, 06:00, 09:00, 12:00, 14:00, 17:00 and 19:00 hours and the levels of their testicular hormones measured by ELISA. A second cohort of 20 men was similarly examined with blood drawn at 19:00 and the following 06:00. Anti-Müllerian hormone levels exhibited a subtle diurnal pattern with a 19:00 peak that was 4.9% higher on average than the 06:00 nadir (p = 0.004). The decrease in anti-Müllerian hormone coincided with a rise in testosterone and InhB, but there was no association between the person-to-person variation in the diurnal patterns of anti-Müllerian hormone and testosterone or Inhibin B. Insulin-like peptide 3 had no diurnal pattern, with only minor sporadic variation between time points being observed in some men. In conclusion, the diurnal and sporadic variation of each testicular hormone is distinct, indicating that the major regulation is at the level of the hormone rather than at the endocrine cell type. Consequently, the balance of the hormones being released by the testes has complex variation during the day. The physiological significance of this will vary depending on which combinations of testicular hormones that the target cells respond to.

No MeSH data available.


Related in: MedlinePlus