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Brassinazole resistant 1 (BZR1)-dependent brassinosteroid signalling pathway leads to ectopic activation of quiescent cell division and suppresses columella stem cell differentiation.

Lee HS, Kim Y, Pham G, Kim JW, Song JH, Lee Y, Hwang YS, Roux SJ, Kim SH - J. Exp. Bot. (2015)

Bottom Line: BR promotes intense nuclear accumulation of BZR1 in the root tip area, and the binding of BZR1 to the promoters of several root development-regulating genes, modulating their expression in the root stem cell niche area.They could also inhibit auxin-dependent distal stem cell differentiation by antagonizing the auxin/WOX5-dependent pathway.In conclusion, BZR1-/BES1-mediated BR signalling pathways show differential effects on the maintenance of root apical meristem activities: they stimulate ectopic QC division while they show opposite effects on the differentiation of distal columella stem cells in a BR concentration- and BZR1-/BES1-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Division of Biological Science and Technology, Yonsei University, Wonju, 220-710, Republic of Korea.

No MeSH data available.


Related in: MedlinePlus

BL regulates expression of PIN genes in a concentration- and BZR1-dependent manner and induces changes in expression domain and subcellular localization of internalization of PIN proteins. (A–C) Quantitative RT-PCR analysis of PIN genes in BL-treated Col-0 and BR-related mutant plants. Plants were grown and PIN gene expressions were analysed for short-term BL-treated plants (A), long-term BL-treated plants (B), and BL-related mutants (C) as described in Fig. 1G–I. (D–E) Short-term effect of BL on subcellular localization of PIN3-GFP protein. The PIN3 reporter plants were grown on 1/2 MS agar media for 7 DAG and treated with BL (10−6 M) for 3h in solution. The images in (D) show GFP expression of representative seedlings for each PIN3 expression pattern of I and II. The graph in (E) presents the frequency of plants with either expression pattern I or II in mock- and BL-treated seedlings. n>50 seedlings for each treatment. PM, plasma membrane; IV, intracellular vesicles. (F) Long-term effect of BL on PINs-GFP protein expression. The PIN reporter seedlings were grown on 1/2 MS agar media for 7 DAG in the presence or absence of BL (10−10 M). Expression pattern indicates number of expressing columella cell layers (CCL) and intensity (HE, high expression; ME, medium expression; LE, low expression) of the corresponding PINs-GFP protein. Numbers in each picture represent the frequency of plants with the corresponding expression pattern in the mock- or BL-treated reporter plants. n>60 seedlings for each treatment. (This figure is available in colour at JXB online.)
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Figure 5: BL regulates expression of PIN genes in a concentration- and BZR1-dependent manner and induces changes in expression domain and subcellular localization of internalization of PIN proteins. (A–C) Quantitative RT-PCR analysis of PIN genes in BL-treated Col-0 and BR-related mutant plants. Plants were grown and PIN gene expressions were analysed for short-term BL-treated plants (A), long-term BL-treated plants (B), and BL-related mutants (C) as described in Fig. 1G–I. (D–E) Short-term effect of BL on subcellular localization of PIN3-GFP protein. The PIN3 reporter plants were grown on 1/2 MS agar media for 7 DAG and treated with BL (10−6 M) for 3h in solution. The images in (D) show GFP expression of representative seedlings for each PIN3 expression pattern of I and II. The graph in (E) presents the frequency of plants with either expression pattern I or II in mock- and BL-treated seedlings. n>50 seedlings for each treatment. PM, plasma membrane; IV, intracellular vesicles. (F) Long-term effect of BL on PINs-GFP protein expression. The PIN reporter seedlings were grown on 1/2 MS agar media for 7 DAG in the presence or absence of BL (10−10 M). Expression pattern indicates number of expressing columella cell layers (CCL) and intensity (HE, high expression; ME, medium expression; LE, low expression) of the corresponding PINs-GFP protein. Numbers in each picture represent the frequency of plants with the corresponding expression pattern in the mock- or BL-treated reporter plants. n>60 seedlings for each treatment. (This figure is available in colour at JXB online.)

Mentions: For propidium iodide (PI) staining (reported in Figs 1 and 5), roots were incubated for 30min in PI (5 μg/ml), rinsed, and mounted in H2O before confocal microscopic observation.


Brassinazole resistant 1 (BZR1)-dependent brassinosteroid signalling pathway leads to ectopic activation of quiescent cell division and suppresses columella stem cell differentiation.

Lee HS, Kim Y, Pham G, Kim JW, Song JH, Lee Y, Hwang YS, Roux SJ, Kim SH - J. Exp. Bot. (2015)

BL regulates expression of PIN genes in a concentration- and BZR1-dependent manner and induces changes in expression domain and subcellular localization of internalization of PIN proteins. (A–C) Quantitative RT-PCR analysis of PIN genes in BL-treated Col-0 and BR-related mutant plants. Plants were grown and PIN gene expressions were analysed for short-term BL-treated plants (A), long-term BL-treated plants (B), and BL-related mutants (C) as described in Fig. 1G–I. (D–E) Short-term effect of BL on subcellular localization of PIN3-GFP protein. The PIN3 reporter plants were grown on 1/2 MS agar media for 7 DAG and treated with BL (10−6 M) for 3h in solution. The images in (D) show GFP expression of representative seedlings for each PIN3 expression pattern of I and II. The graph in (E) presents the frequency of plants with either expression pattern I or II in mock- and BL-treated seedlings. n>50 seedlings for each treatment. PM, plasma membrane; IV, intracellular vesicles. (F) Long-term effect of BL on PINs-GFP protein expression. The PIN reporter seedlings were grown on 1/2 MS agar media for 7 DAG in the presence or absence of BL (10−10 M). Expression pattern indicates number of expressing columella cell layers (CCL) and intensity (HE, high expression; ME, medium expression; LE, low expression) of the corresponding PINs-GFP protein. Numbers in each picture represent the frequency of plants with the corresponding expression pattern in the mock- or BL-treated reporter plants. n>60 seedlings for each treatment. (This figure is available in colour at JXB online.)
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Related In: Results  -  Collection

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Figure 5: BL regulates expression of PIN genes in a concentration- and BZR1-dependent manner and induces changes in expression domain and subcellular localization of internalization of PIN proteins. (A–C) Quantitative RT-PCR analysis of PIN genes in BL-treated Col-0 and BR-related mutant plants. Plants were grown and PIN gene expressions were analysed for short-term BL-treated plants (A), long-term BL-treated plants (B), and BL-related mutants (C) as described in Fig. 1G–I. (D–E) Short-term effect of BL on subcellular localization of PIN3-GFP protein. The PIN3 reporter plants were grown on 1/2 MS agar media for 7 DAG and treated with BL (10−6 M) for 3h in solution. The images in (D) show GFP expression of representative seedlings for each PIN3 expression pattern of I and II. The graph in (E) presents the frequency of plants with either expression pattern I or II in mock- and BL-treated seedlings. n>50 seedlings for each treatment. PM, plasma membrane; IV, intracellular vesicles. (F) Long-term effect of BL on PINs-GFP protein expression. The PIN reporter seedlings were grown on 1/2 MS agar media for 7 DAG in the presence or absence of BL (10−10 M). Expression pattern indicates number of expressing columella cell layers (CCL) and intensity (HE, high expression; ME, medium expression; LE, low expression) of the corresponding PINs-GFP protein. Numbers in each picture represent the frequency of plants with the corresponding expression pattern in the mock- or BL-treated reporter plants. n>60 seedlings for each treatment. (This figure is available in colour at JXB online.)
Mentions: For propidium iodide (PI) staining (reported in Figs 1 and 5), roots were incubated for 30min in PI (5 μg/ml), rinsed, and mounted in H2O before confocal microscopic observation.

Bottom Line: BR promotes intense nuclear accumulation of BZR1 in the root tip area, and the binding of BZR1 to the promoters of several root development-regulating genes, modulating their expression in the root stem cell niche area.They could also inhibit auxin-dependent distal stem cell differentiation by antagonizing the auxin/WOX5-dependent pathway.In conclusion, BZR1-/BES1-mediated BR signalling pathways show differential effects on the maintenance of root apical meristem activities: they stimulate ectopic QC division while they show opposite effects on the differentiation of distal columella stem cells in a BR concentration- and BZR1-/BES1-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Division of Biological Science and Technology, Yonsei University, Wonju, 220-710, Republic of Korea.

No MeSH data available.


Related in: MedlinePlus