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The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots.

Chen CY, Schmidt W - J. Exp. Bot. (2015)

Bottom Line: The data show that in an intricate interplay between the three MYBs regulate several developmental, physiological and metabolic processes that are putatively located in different tissues.Control of gene activity is partly mediated through the regulation of potential antisense transcripts.The current dataset extends the known functions of R3 MYB proteins, provides a suite of novel candidates with critical function in root hair development under both control and Pi-deficient conditions, and challenges the definition of genetic redundancy by demonstrating that environmental perturbations may confer specific functions to orthologous proteins that could have similar roles under control conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.

No MeSH data available.


Related in: MedlinePlus

Regulation of transition metal transporters by CPC, ETC1 and TRY. The transport proteins are likely localized in different cell types and tissues.
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Figure 7: Regulation of transition metal transporters by CPC, ETC1 and TRY. The transport proteins are likely localized in different cell types and tissues.

Mentions: To further decipher processes that are regulated by the three TFs under control and/or low Pi conditions, we considered genes in which the ratio (wild type low Pi/wild type control)/(mutant low Pi/mutant control) was more than 2-fold changed. Interestingly, several genes encoding transition metal and Pi transporters regulated by CPC and ETC1 fulfilled this criterion (Fig. 7). In particular, genes encoding plasma membrane (PM)-bound Fe2+/Zn2+ transporters were positively regulated either by CPC alone (ZIP3, ZIP9), or by both CPC and ETC1 (HMA2). In the wild type, the expression of Zn2+ transporters was strongly reduced upon Pi starvation, a response that was less pronounced in cpc and etc1 mutants. In addition, under control conditions, expression levels of these genes were 2–3-fold lower in the mutants than in the wild type. In contrast, the expression of the Fe2+ transporters IRT1 and IRT2 was higher in cpc and etc1 mutants than in the wild type when grown on Pi-replete media. Pi starvation leads to an increase in Fe concentrations for reasons that are still under debate, causing a pronounced down-regulation of the Fe2+ transporters (Misson et al., 2005; Lan et al., 2012). This down-regulation was more pronounced in cpc and etc1 mutants. It thus appears that under Pi-replete conditions the expression of Zn2+ transporters is reduced in the mutants relative to the wild type, while that of Fe2+ transporters was higher. Under low Pi conditions, this relationship is reversed, i.e. a slightly higher (but statistically insignificant) expression of Zn2+ transporters and a lower expression of Fe2+transporters in the mutants. In support of an altered Zn2+ homeostasis in response to Pi starvation, the PM-bound Zn2+ exporter PCR2 was highly induced upon Pi starvation in roots of wild-type plants. In all three mutants, the induction of PCR2 was lower than in the wild type, but the difference did not reach the 2-fold threshold used here.


The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots.

Chen CY, Schmidt W - J. Exp. Bot. (2015)

Regulation of transition metal transporters by CPC, ETC1 and TRY. The transport proteins are likely localized in different cell types and tissues.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507782&req=5

Figure 7: Regulation of transition metal transporters by CPC, ETC1 and TRY. The transport proteins are likely localized in different cell types and tissues.
Mentions: To further decipher processes that are regulated by the three TFs under control and/or low Pi conditions, we considered genes in which the ratio (wild type low Pi/wild type control)/(mutant low Pi/mutant control) was more than 2-fold changed. Interestingly, several genes encoding transition metal and Pi transporters regulated by CPC and ETC1 fulfilled this criterion (Fig. 7). In particular, genes encoding plasma membrane (PM)-bound Fe2+/Zn2+ transporters were positively regulated either by CPC alone (ZIP3, ZIP9), or by both CPC and ETC1 (HMA2). In the wild type, the expression of Zn2+ transporters was strongly reduced upon Pi starvation, a response that was less pronounced in cpc and etc1 mutants. In addition, under control conditions, expression levels of these genes were 2–3-fold lower in the mutants than in the wild type. In contrast, the expression of the Fe2+ transporters IRT1 and IRT2 was higher in cpc and etc1 mutants than in the wild type when grown on Pi-replete media. Pi starvation leads to an increase in Fe concentrations for reasons that are still under debate, causing a pronounced down-regulation of the Fe2+ transporters (Misson et al., 2005; Lan et al., 2012). This down-regulation was more pronounced in cpc and etc1 mutants. It thus appears that under Pi-replete conditions the expression of Zn2+ transporters is reduced in the mutants relative to the wild type, while that of Fe2+ transporters was higher. Under low Pi conditions, this relationship is reversed, i.e. a slightly higher (but statistically insignificant) expression of Zn2+ transporters and a lower expression of Fe2+transporters in the mutants. In support of an altered Zn2+ homeostasis in response to Pi starvation, the PM-bound Zn2+ exporter PCR2 was highly induced upon Pi starvation in roots of wild-type plants. In all three mutants, the induction of PCR2 was lower than in the wild type, but the difference did not reach the 2-fold threshold used here.

Bottom Line: The data show that in an intricate interplay between the three MYBs regulate several developmental, physiological and metabolic processes that are putatively located in different tissues.Control of gene activity is partly mediated through the regulation of potential antisense transcripts.The current dataset extends the known functions of R3 MYB proteins, provides a suite of novel candidates with critical function in root hair development under both control and Pi-deficient conditions, and challenges the definition of genetic redundancy by demonstrating that environmental perturbations may confer specific functions to orthologous proteins that could have similar roles under control conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.

No MeSH data available.


Related in: MedlinePlus