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The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots.

Chen CY, Schmidt W - J. Exp. Bot. (2015)

Bottom Line: The data show that in an intricate interplay between the three MYBs regulate several developmental, physiological and metabolic processes that are putatively located in different tissues.Control of gene activity is partly mediated through the regulation of potential antisense transcripts.The current dataset extends the known functions of R3 MYB proteins, provides a suite of novel candidates with critical function in root hair development under both control and Pi-deficient conditions, and challenges the definition of genetic redundancy by demonstrating that environmental perturbations may confer specific functions to orthologous proteins that could have similar roles under control conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.

No MeSH data available.


Related in: MedlinePlus

Effect of Pi deficiency on the expression of R3 MYB protein. (A) Abundance changes in the transcripts of CPC, ETC1, ETC3 and TRY determined by RNA-seq analysis. Values are given in RPKM. (B, C) CPC promoter activity. Cross-sections are from the meristematic region of pCPC-GUS plants grown under (B) control and (C) Pi-deficient conditions. (This figure is available in colour at JXB online.)
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Figure 3: Effect of Pi deficiency on the expression of R3 MYB protein. (A) Abundance changes in the transcripts of CPC, ETC1, ETC3 and TRY determined by RNA-seq analysis. Values are given in RPKM. (B, C) CPC promoter activity. Cross-sections are from the meristematic region of pCPC-GUS plants grown under (B) control and (C) Pi-deficient conditions. (This figure is available in colour at JXB online.)

Mentions: A previous RNA-seq study revealed that of the genes that determine root epidermal cell fate only ETC1 and ETC3 were Pi-responsive, being significantly up-regulated upon Pi starvation (Lan et al., 2012). In the present study, a relative small, non-significant increase in ETC1 expression was observed (Fig. 3A). This difference is likely due to differences in growth conditions between the two studies [transfer to Pi-free media in Lan et al. (2012) versus growth on low Pi media in this study]. The expression of CPC increased ~2-fold when plants were grown on low Pi media, TRY transcript levels were not affected by the Pi regime. Notably, ETC3 expression was low under Pi-replete conditions but was strongly induced by low Pi, resulting in a robust change in the relative abundance of the R3 MYB transcripts (Fig. 3A). Another homologue of ETC1, ETC2 was not expressed in roots. Lack of detectable ETC2 and ETC3 transcript in (Pi-replete) roots was reported previously (Kirik et al., 2004b; Simon et al., 2007).


The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots.

Chen CY, Schmidt W - J. Exp. Bot. (2015)

Effect of Pi deficiency on the expression of R3 MYB protein. (A) Abundance changes in the transcripts of CPC, ETC1, ETC3 and TRY determined by RNA-seq analysis. Values are given in RPKM. (B, C) CPC promoter activity. Cross-sections are from the meristematic region of pCPC-GUS plants grown under (B) control and (C) Pi-deficient conditions. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507782&req=5

Figure 3: Effect of Pi deficiency on the expression of R3 MYB protein. (A) Abundance changes in the transcripts of CPC, ETC1, ETC3 and TRY determined by RNA-seq analysis. Values are given in RPKM. (B, C) CPC promoter activity. Cross-sections are from the meristematic region of pCPC-GUS plants grown under (B) control and (C) Pi-deficient conditions. (This figure is available in colour at JXB online.)
Mentions: A previous RNA-seq study revealed that of the genes that determine root epidermal cell fate only ETC1 and ETC3 were Pi-responsive, being significantly up-regulated upon Pi starvation (Lan et al., 2012). In the present study, a relative small, non-significant increase in ETC1 expression was observed (Fig. 3A). This difference is likely due to differences in growth conditions between the two studies [transfer to Pi-free media in Lan et al. (2012) versus growth on low Pi media in this study]. The expression of CPC increased ~2-fold when plants were grown on low Pi media, TRY transcript levels were not affected by the Pi regime. Notably, ETC3 expression was low under Pi-replete conditions but was strongly induced by low Pi, resulting in a robust change in the relative abundance of the R3 MYB transcripts (Fig. 3A). Another homologue of ETC1, ETC2 was not expressed in roots. Lack of detectable ETC2 and ETC3 transcript in (Pi-replete) roots was reported previously (Kirik et al., 2004b; Simon et al., 2007).

Bottom Line: The data show that in an intricate interplay between the three MYBs regulate several developmental, physiological and metabolic processes that are putatively located in different tissues.Control of gene activity is partly mediated through the regulation of potential antisense transcripts.The current dataset extends the known functions of R3 MYB proteins, provides a suite of novel candidates with critical function in root hair development under both control and Pi-deficient conditions, and challenges the definition of genetic redundancy by demonstrating that environmental perturbations may confer specific functions to orthologous proteins that could have similar roles under control conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.

No MeSH data available.


Related in: MedlinePlus