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The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots.

Chen CY, Schmidt W - J. Exp. Bot. (2015)

Bottom Line: The data show that in an intricate interplay between the three MYBs regulate several developmental, physiological and metabolic processes that are putatively located in different tissues.Control of gene activity is partly mediated through the regulation of potential antisense transcripts.The current dataset extends the known functions of R3 MYB proteins, provides a suite of novel candidates with critical function in root hair development under both control and Pi-deficient conditions, and challenges the definition of genetic redundancy by demonstrating that environmental perturbations may confer specific functions to orthologous proteins that could have similar roles under control conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.

No MeSH data available.


Related in: MedlinePlus

Genes differentially expressed between cpc, etc1 and try mutants and the wild-type plants. Pie charts show genes that are preferentially expressed in root hairs (RH; Lan et al., 2013) and in the phosphate starvation response (PSR) genes. Numbers represent gene counts. In the pie charts, red and blue colour represents the percentage of up- and down-regulated genes, respectively. (This figure is available in colour at JXB online.)
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Figure 2: Genes differentially expressed between cpc, etc1 and try mutants and the wild-type plants. Pie charts show genes that are preferentially expressed in root hairs (RH; Lan et al., 2013) and in the phosphate starvation response (PSR) genes. Numbers represent gene counts. In the pie charts, red and blue colour represents the percentage of up- and down-regulated genes, respectively. (This figure is available in colour at JXB online.)

Mentions: To identify genes that are regulated by one or more of the TFs under investigation, we surveyed the transcriptome of roots from wild-type and cpc, etc1 and try plants using RNA-seq. Sequencing was performed in triplicates with on average ten million reads per library (Supplementary Table S1), and genes that showed a deviation in transcript abundance from the wild type greater than or equal to 1.5-fold with P<0.05 were defined as differentially expressed. Validation of a subset of the differentially expressed genes by qRT-PCR showed a generally good agreement between the two methods (Supplementary Fig. S2, Supplementary Table S4). For a relatively large suite of genes at least one of the three R3 MYBs was required for wild type-like expression. Under control conditions, the largest subset was dependent on CPC expression (723 genes), followed by TRY (630) and ETC1 (184) (Fig. 2, Supplementary Table S2).


The paralogous R3 MYB proteins CAPRICE, TRIPTYCHON and ENHANCER OF TRY AND CPC1 play pleiotropic and partly non-redundant roles in the phosphate starvation response of Arabidopsis roots.

Chen CY, Schmidt W - J. Exp. Bot. (2015)

Genes differentially expressed between cpc, etc1 and try mutants and the wild-type plants. Pie charts show genes that are preferentially expressed in root hairs (RH; Lan et al., 2013) and in the phosphate starvation response (PSR) genes. Numbers represent gene counts. In the pie charts, red and blue colour represents the percentage of up- and down-regulated genes, respectively. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507782&req=5

Figure 2: Genes differentially expressed between cpc, etc1 and try mutants and the wild-type plants. Pie charts show genes that are preferentially expressed in root hairs (RH; Lan et al., 2013) and in the phosphate starvation response (PSR) genes. Numbers represent gene counts. In the pie charts, red and blue colour represents the percentage of up- and down-regulated genes, respectively. (This figure is available in colour at JXB online.)
Mentions: To identify genes that are regulated by one or more of the TFs under investigation, we surveyed the transcriptome of roots from wild-type and cpc, etc1 and try plants using RNA-seq. Sequencing was performed in triplicates with on average ten million reads per library (Supplementary Table S1), and genes that showed a deviation in transcript abundance from the wild type greater than or equal to 1.5-fold with P<0.05 were defined as differentially expressed. Validation of a subset of the differentially expressed genes by qRT-PCR showed a generally good agreement between the two methods (Supplementary Fig. S2, Supplementary Table S4). For a relatively large suite of genes at least one of the three R3 MYBs was required for wild type-like expression. Under control conditions, the largest subset was dependent on CPC expression (723 genes), followed by TRY (630) and ETC1 (184) (Fig. 2, Supplementary Table S2).

Bottom Line: The data show that in an intricate interplay between the three MYBs regulate several developmental, physiological and metabolic processes that are putatively located in different tissues.Control of gene activity is partly mediated through the regulation of potential antisense transcripts.The current dataset extends the known functions of R3 MYB proteins, provides a suite of novel candidates with critical function in root hair development under both control and Pi-deficient conditions, and challenges the definition of genetic redundancy by demonstrating that environmental perturbations may confer specific functions to orthologous proteins that could have similar roles under control conditions.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan, and National Chung-Hsing University, Taichung, Taiwan Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan.

No MeSH data available.


Related in: MedlinePlus