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Characterization, localization, and seasonal changes of the sucrose transporter FeSUT1 in the phloem of Fraxinus excelsior.

Öner-Sieben S, Rappl C, Sauer N, Stadler R, Lohaus G - J. Exp. Bot. (2015)

Bottom Line: The localization and expression pattern point towards functions of FeSUT1 in phloem loading of sucrose as well as in sucrose retrieval.The elevated expression level of FeSUT1 indicated an increased apoplastic carbon export activity from the leaves during spring and late autumn.It is hypothesized that the importance of apoplastic loading is high under low-sucrose conditions and that the availability of two different phloem-loading mechanisms confers advantages for temperate woody species like F. excelsior.

View Article: PubMed Central - PubMed

Affiliation: Molekulare Pflanzenforschung/Pflanzenbiochemie (Botanik), Bergische Universität Wuppertal, Gaußstraße 20, D-42119 Wuppertal, Germany.

No MeSH data available.


Related in: MedlinePlus

Localization of FeSUT1 in midribs and minor veins of F. excelsior leaves by fluorescence detection with anti-FeSUT1 antibody. Leaves were taken from ~30-year-old trees. Yellow or red staining shows autofluorescence of phenolics in lignified cells, green staining shows FeSUT1 localization. Specific anti-FeSUT1 antibody reaction is indicated by green Alexa Fluor® labelling. (A) Overview of a cross section of a leaf midrib treated with anti-FeSUT1-antiserum. Labelling was seen in the circularly arranged transport phloem. (B) Higher magnification of the transport phloem in the leaf midrib imaged in phase-contrast. Irregular shaped cells of the phloem were stained. (C) Section of the midrib incubated with antiserum prior to immunization as negative control imaged in phase-contrast. (D) Longitudinal sections of a vascular bundle of the midrib. Green staining shows FeSUT1 localization. Blue labelling shows detection of callose in sieve plates with aniline blue. (E) Phase-contrast image of the FeSUT1-localization in the minor veins of F. excelsior. Labelled cells (arrow) are SEs or CCs rather than ICs. (F) Same minor vein image without fluorescence light. (G) Electron micrograph of a minor vein of F. excelsior highlighting the regular arrangement of cells. SEs (arrowhead) and CCs are located in the central part of the minor veins whereas the ICs are laterally localized. Scale bars = (A) 100 µm; (B, C) 25 µm; (D) 20 µm; (E, F) 7.5 µm; (G) 5 µm. PP, phloem parenchyma cell; X, xylem.
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Figure 3: Localization of FeSUT1 in midribs and minor veins of F. excelsior leaves by fluorescence detection with anti-FeSUT1 antibody. Leaves were taken from ~30-year-old trees. Yellow or red staining shows autofluorescence of phenolics in lignified cells, green staining shows FeSUT1 localization. Specific anti-FeSUT1 antibody reaction is indicated by green Alexa Fluor® labelling. (A) Overview of a cross section of a leaf midrib treated with anti-FeSUT1-antiserum. Labelling was seen in the circularly arranged transport phloem. (B) Higher magnification of the transport phloem in the leaf midrib imaged in phase-contrast. Irregular shaped cells of the phloem were stained. (C) Section of the midrib incubated with antiserum prior to immunization as negative control imaged in phase-contrast. (D) Longitudinal sections of a vascular bundle of the midrib. Green staining shows FeSUT1 localization. Blue labelling shows detection of callose in sieve plates with aniline blue. (E) Phase-contrast image of the FeSUT1-localization in the minor veins of F. excelsior. Labelled cells (arrow) are SEs or CCs rather than ICs. (F) Same minor vein image without fluorescence light. (G) Electron micrograph of a minor vein of F. excelsior highlighting the regular arrangement of cells. SEs (arrowhead) and CCs are located in the central part of the minor veins whereas the ICs are laterally localized. Scale bars = (A) 100 µm; (B, C) 25 µm; (D) 20 µm; (E, F) 7.5 µm; (G) 5 µm. PP, phloem parenchyma cell; X, xylem.

Mentions: To localize FeSUT1 on the cellular level, anti-FeSUT1-antiserum was used on sections from leaves of F. excelsior. Figure 3A, B shows cross-sections of the midrib of F. excelsior with anti-FeSUT1-antiserum-derived fluorescence signal in the transport phloem. Pre-immune serum was used as a negative control (Fig. 3C). Autofluorescence of cells containing phenolic compounds is shown in yellow or red.


Characterization, localization, and seasonal changes of the sucrose transporter FeSUT1 in the phloem of Fraxinus excelsior.

Öner-Sieben S, Rappl C, Sauer N, Stadler R, Lohaus G - J. Exp. Bot. (2015)

Localization of FeSUT1 in midribs and minor veins of F. excelsior leaves by fluorescence detection with anti-FeSUT1 antibody. Leaves were taken from ~30-year-old trees. Yellow or red staining shows autofluorescence of phenolics in lignified cells, green staining shows FeSUT1 localization. Specific anti-FeSUT1 antibody reaction is indicated by green Alexa Fluor® labelling. (A) Overview of a cross section of a leaf midrib treated with anti-FeSUT1-antiserum. Labelling was seen in the circularly arranged transport phloem. (B) Higher magnification of the transport phloem in the leaf midrib imaged in phase-contrast. Irregular shaped cells of the phloem were stained. (C) Section of the midrib incubated with antiserum prior to immunization as negative control imaged in phase-contrast. (D) Longitudinal sections of a vascular bundle of the midrib. Green staining shows FeSUT1 localization. Blue labelling shows detection of callose in sieve plates with aniline blue. (E) Phase-contrast image of the FeSUT1-localization in the minor veins of F. excelsior. Labelled cells (arrow) are SEs or CCs rather than ICs. (F) Same minor vein image without fluorescence light. (G) Electron micrograph of a minor vein of F. excelsior highlighting the regular arrangement of cells. SEs (arrowhead) and CCs are located in the central part of the minor veins whereas the ICs are laterally localized. Scale bars = (A) 100 µm; (B, C) 25 µm; (D) 20 µm; (E, F) 7.5 µm; (G) 5 µm. PP, phloem parenchyma cell; X, xylem.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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Figure 3: Localization of FeSUT1 in midribs and minor veins of F. excelsior leaves by fluorescence detection with anti-FeSUT1 antibody. Leaves were taken from ~30-year-old trees. Yellow or red staining shows autofluorescence of phenolics in lignified cells, green staining shows FeSUT1 localization. Specific anti-FeSUT1 antibody reaction is indicated by green Alexa Fluor® labelling. (A) Overview of a cross section of a leaf midrib treated with anti-FeSUT1-antiserum. Labelling was seen in the circularly arranged transport phloem. (B) Higher magnification of the transport phloem in the leaf midrib imaged in phase-contrast. Irregular shaped cells of the phloem were stained. (C) Section of the midrib incubated with antiserum prior to immunization as negative control imaged in phase-contrast. (D) Longitudinal sections of a vascular bundle of the midrib. Green staining shows FeSUT1 localization. Blue labelling shows detection of callose in sieve plates with aniline blue. (E) Phase-contrast image of the FeSUT1-localization in the minor veins of F. excelsior. Labelled cells (arrow) are SEs or CCs rather than ICs. (F) Same minor vein image without fluorescence light. (G) Electron micrograph of a minor vein of F. excelsior highlighting the regular arrangement of cells. SEs (arrowhead) and CCs are located in the central part of the minor veins whereas the ICs are laterally localized. Scale bars = (A) 100 µm; (B, C) 25 µm; (D) 20 µm; (E, F) 7.5 µm; (G) 5 µm. PP, phloem parenchyma cell; X, xylem.
Mentions: To localize FeSUT1 on the cellular level, anti-FeSUT1-antiserum was used on sections from leaves of F. excelsior. Figure 3A, B shows cross-sections of the midrib of F. excelsior with anti-FeSUT1-antiserum-derived fluorescence signal in the transport phloem. Pre-immune serum was used as a negative control (Fig. 3C). Autofluorescence of cells containing phenolic compounds is shown in yellow or red.

Bottom Line: The localization and expression pattern point towards functions of FeSUT1 in phloem loading of sucrose as well as in sucrose retrieval.The elevated expression level of FeSUT1 indicated an increased apoplastic carbon export activity from the leaves during spring and late autumn.It is hypothesized that the importance of apoplastic loading is high under low-sucrose conditions and that the availability of two different phloem-loading mechanisms confers advantages for temperate woody species like F. excelsior.

View Article: PubMed Central - PubMed

Affiliation: Molekulare Pflanzenforschung/Pflanzenbiochemie (Botanik), Bergische Universität Wuppertal, Gaußstraße 20, D-42119 Wuppertal, Germany.

No MeSH data available.


Related in: MedlinePlus