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Synchrotron-based X-ray absorption near-edge spectroscopy imaging for laterally resolved speciation of selenium in fresh roots and leaves of wheat and rice.

Wang P, Menzies NW, Lombi E, McKenna BA, James S, Tang C, Kopittke PM - J. Exp. Bot. (2015)

Bottom Line: Indeed, even in the rhizodermis which is exposed directly to the bulk solution, only 12-31% of the Se was present as uncomplexed selenate.In a similar manner, for plants exposed to selenite, the Se was efficiently converted to C-Se-C compounds within the roots, with only a small proportion of uncomplexed selenite observed within the outer root tissues.This resulted in a substantial decrease in translocation of Se from the roots to leaves of selenite-exposed plants.

View Article: PubMed Central - PubMed

Affiliation: The University of Queensland, School of Agriculture and Food Sciences, St. Lucia, Queensland, 4072, Australia p.wang3@uq.edu.au.

No MeSH data available.


Related in: MedlinePlus

The mature primary root (3.0mm from the tip) of rice (Oryza sativa L.), with the lateral root primordium which initiates from the primary root. (A and B) Elemental survey maps showing total Se distribution collected in the ‘pre-XANES survey scan’ followed by fluorescence-XANES imaging (‘XANES imaging scan’), with the white box (0.27 mm×0.15mm) indicating the area examined by XANES imaging. (C) The spatial distribution of two pixel populations (outer tissues and hot spot) identified by comparing energy intensities. (D Normalized Se K-edge XANES spectra corresponding to the two pixel populations ‘hot spot’ and ‘outer tissues’ shown in (C) plus the spectrum for MeSeCys.
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Figure 3: The mature primary root (3.0mm from the tip) of rice (Oryza sativa L.), with the lateral root primordium which initiates from the primary root. (A and B) Elemental survey maps showing total Se distribution collected in the ‘pre-XANES survey scan’ followed by fluorescence-XANES imaging (‘XANES imaging scan’), with the white box (0.27 mm×0.15mm) indicating the area examined by XANES imaging. (C) The spatial distribution of two pixel populations (outer tissues and hot spot) identified by comparing energy intensities. (D Normalized Se K-edge XANES spectra corresponding to the two pixel populations ‘hot spot’ and ‘outer tissues’ shown in (C) plus the spectrum for MeSeCys.

Mentions: Data are presented for the ‘outer’, ‘middle’, and ‘inner’ tissues (see Figs 1, 2, 4, 5) and the lateral root primordia (Fig. 3).


Synchrotron-based X-ray absorption near-edge spectroscopy imaging for laterally resolved speciation of selenium in fresh roots and leaves of wheat and rice.

Wang P, Menzies NW, Lombi E, McKenna BA, James S, Tang C, Kopittke PM - J. Exp. Bot. (2015)

The mature primary root (3.0mm from the tip) of rice (Oryza sativa L.), with the lateral root primordium which initiates from the primary root. (A and B) Elemental survey maps showing total Se distribution collected in the ‘pre-XANES survey scan’ followed by fluorescence-XANES imaging (‘XANES imaging scan’), with the white box (0.27 mm×0.15mm) indicating the area examined by XANES imaging. (C) The spatial distribution of two pixel populations (outer tissues and hot spot) identified by comparing energy intensities. (D Normalized Se K-edge XANES spectra corresponding to the two pixel populations ‘hot spot’ and ‘outer tissues’ shown in (C) plus the spectrum for MeSeCys.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507780&req=5

Figure 3: The mature primary root (3.0mm from the tip) of rice (Oryza sativa L.), with the lateral root primordium which initiates from the primary root. (A and B) Elemental survey maps showing total Se distribution collected in the ‘pre-XANES survey scan’ followed by fluorescence-XANES imaging (‘XANES imaging scan’), with the white box (0.27 mm×0.15mm) indicating the area examined by XANES imaging. (C) The spatial distribution of two pixel populations (outer tissues and hot spot) identified by comparing energy intensities. (D Normalized Se K-edge XANES spectra corresponding to the two pixel populations ‘hot spot’ and ‘outer tissues’ shown in (C) plus the spectrum for MeSeCys.
Mentions: Data are presented for the ‘outer’, ‘middle’, and ‘inner’ tissues (see Figs 1, 2, 4, 5) and the lateral root primordia (Fig. 3).

Bottom Line: Indeed, even in the rhizodermis which is exposed directly to the bulk solution, only 12-31% of the Se was present as uncomplexed selenate.In a similar manner, for plants exposed to selenite, the Se was efficiently converted to C-Se-C compounds within the roots, with only a small proportion of uncomplexed selenite observed within the outer root tissues.This resulted in a substantial decrease in translocation of Se from the roots to leaves of selenite-exposed plants.

View Article: PubMed Central - PubMed

Affiliation: The University of Queensland, School of Agriculture and Food Sciences, St. Lucia, Queensland, 4072, Australia p.wang3@uq.edu.au.

No MeSH data available.


Related in: MedlinePlus