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Effect of herbicide resistance endowing Ile-1781-Leu and Asp-2078-Gly ACCase gene mutations on ACCase kinetics and growth traits in Lolium rigidum.

Vila-Aiub MM, Yu Q, Han H, Powles SB - J. Exp. Bot. (2015)

Bottom Line: Both the negligible and significant growth reductions associated, respectively, with the Ile-1781-Leu and Asp-2078-Gly resistance mutations correlated with their impact on ACCase activity.Whereas the Ile-1781-Leu mutation showed no pleiotropic effects on ACCase kinetics, the Asp-2078-Gly mutation led to a significant reduction in ACCase activity.Similar effects of these two particular ACCase mutations on the ACCase activity of Alopecurus myosuroides were also confirmed.

View Article: PubMed Central - PubMed

Affiliation: Australian Herbicide Resistance Initiative (AHRI) - School of Plant Biology, University of Western Australia, WA, 6009, Australia IFEVA - CONICET - Facultad de Agronomía, Universidad de Buenos Aires (UBA), Buenos Aires, 1417, Argentina vila@ifeva.edu.ar.

No MeSH data available.


Related in: MedlinePlus

Above-ground and root biomass and leaf area produced by L. rigidum plants of susceptible wild-type ACCase (SS) (white bar) and homozygous resistant (RR) (Ile-1781-Leu, Asp-2078-Gly) mutants after 24 days of growth in the absence of plant resource competition. Growth traits corresponding to plants carrying the ACCase herbicide–susceptible alleles are mean estimates resulting from all susceptible wild type populations (S1, S2, S3 and S4). Vertical bars denote SE of the mean (n = 15). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.
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Figure 2: Above-ground and root biomass and leaf area produced by L. rigidum plants of susceptible wild-type ACCase (SS) (white bar) and homozygous resistant (RR) (Ile-1781-Leu, Asp-2078-Gly) mutants after 24 days of growth in the absence of plant resource competition. Growth traits corresponding to plants carrying the ACCase herbicide–susceptible alleles are mean estimates resulting from all susceptible wild type populations (S1, S2, S3 and S4). Vertical bars denote SE of the mean (n = 15). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.

Mentions: Estimated RGR, NAR, and LAR parameters did not differ among the four ACCase herbicide–susceptible populations (P > 0.05) (Table 2). Therefore, a mean growth parameter was calculated for all susceptible populations and used as a reference estimate for further comparisons. Growth analysis revealed that plants homozygous for the Ile-1781-Leu mutation exhibited similar RGR, NAR, and NAR growth parameters to herbicide-susceptible plants (Table 2). At the end of the growing period, the Ile-1781-Leu mutants showed above-ground and root biomass and leaf area similar to susceptible wild-type plants (Fig. 2).


Effect of herbicide resistance endowing Ile-1781-Leu and Asp-2078-Gly ACCase gene mutations on ACCase kinetics and growth traits in Lolium rigidum.

Vila-Aiub MM, Yu Q, Han H, Powles SB - J. Exp. Bot. (2015)

Above-ground and root biomass and leaf area produced by L. rigidum plants of susceptible wild-type ACCase (SS) (white bar) and homozygous resistant (RR) (Ile-1781-Leu, Asp-2078-Gly) mutants after 24 days of growth in the absence of plant resource competition. Growth traits corresponding to plants carrying the ACCase herbicide–susceptible alleles are mean estimates resulting from all susceptible wild type populations (S1, S2, S3 and S4). Vertical bars denote SE of the mean (n = 15). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507778&req=5

Figure 2: Above-ground and root biomass and leaf area produced by L. rigidum plants of susceptible wild-type ACCase (SS) (white bar) and homozygous resistant (RR) (Ile-1781-Leu, Asp-2078-Gly) mutants after 24 days of growth in the absence of plant resource competition. Growth traits corresponding to plants carrying the ACCase herbicide–susceptible alleles are mean estimates resulting from all susceptible wild type populations (S1, S2, S3 and S4). Vertical bars denote SE of the mean (n = 15). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.
Mentions: Estimated RGR, NAR, and LAR parameters did not differ among the four ACCase herbicide–susceptible populations (P > 0.05) (Table 2). Therefore, a mean growth parameter was calculated for all susceptible populations and used as a reference estimate for further comparisons. Growth analysis revealed that plants homozygous for the Ile-1781-Leu mutation exhibited similar RGR, NAR, and NAR growth parameters to herbicide-susceptible plants (Table 2). At the end of the growing period, the Ile-1781-Leu mutants showed above-ground and root biomass and leaf area similar to susceptible wild-type plants (Fig. 2).

Bottom Line: Both the negligible and significant growth reductions associated, respectively, with the Ile-1781-Leu and Asp-2078-Gly resistance mutations correlated with their impact on ACCase activity.Whereas the Ile-1781-Leu mutation showed no pleiotropic effects on ACCase kinetics, the Asp-2078-Gly mutation led to a significant reduction in ACCase activity.Similar effects of these two particular ACCase mutations on the ACCase activity of Alopecurus myosuroides were also confirmed.

View Article: PubMed Central - PubMed

Affiliation: Australian Herbicide Resistance Initiative (AHRI) - School of Plant Biology, University of Western Australia, WA, 6009, Australia IFEVA - CONICET - Facultad de Agronomía, Universidad de Buenos Aires (UBA), Buenos Aires, 1417, Argentina vila@ifeva.edu.ar.

No MeSH data available.


Related in: MedlinePlus