Limits...
Effect of herbicide resistance endowing Ile-1781-Leu and Asp-2078-Gly ACCase gene mutations on ACCase kinetics and growth traits in Lolium rigidum.

Vila-Aiub MM, Yu Q, Han H, Powles SB - J. Exp. Bot. (2015)

Bottom Line: Both the negligible and significant growth reductions associated, respectively, with the Ile-1781-Leu and Asp-2078-Gly resistance mutations correlated with their impact on ACCase activity.Whereas the Ile-1781-Leu mutation showed no pleiotropic effects on ACCase kinetics, the Asp-2078-Gly mutation led to a significant reduction in ACCase activity.Similar effects of these two particular ACCase mutations on the ACCase activity of Alopecurus myosuroides were also confirmed.

View Article: PubMed Central - PubMed

Affiliation: Australian Herbicide Resistance Initiative (AHRI) - School of Plant Biology, University of Western Australia, WA, 6009, Australia IFEVA - CONICET - Facultad de Agronomía, Universidad de Buenos Aires (UBA), Buenos Aires, 1417, Argentina vila@ifeva.edu.ar.

No MeSH data available.


Related in: MedlinePlus

Extractable ACCase activity measured from partially purified enzyme extracts from the three- to four-leaf stage (A) L. rigidum and (B) A. myosuroides plants of ACCase herbicide–susceptible (SS), wild type (white bar), and homozygous resistant (RR) mutants (Ile-1781-Leu and Asp-2078-Gly). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507778&req=5

Figure 1: Extractable ACCase activity measured from partially purified enzyme extracts from the three- to four-leaf stage (A) L. rigidum and (B) A. myosuroides plants of ACCase herbicide–susceptible (SS), wild type (white bar), and homozygous resistant (RR) mutants (Ile-1781-Leu and Asp-2078-Gly). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.

Mentions: The extractable ACCase activity was the same in L. rigidum plants homozygous for the Ile-1781-Leu mutation as in the wild-type herbicide-susceptible plants (Fig. 1A). In contrast, a significantly lower specific ACCase activity was found in plants homozygous for the Asp-2078-Gly (Fig. 1A). Plants with the Asp-2078-Gly mutation showed only 70% extractable ACCase activity (specific activity) when compared to susceptible wild-type plants. Similarly in A. myosuroides, plants homozygous for the Asp-2078-Gly mutation displayed a significantly reduced (40%) ACCase activity, whereas plants with the Ile-1781-Leu mutation showed no change in ACCase activity, relative to the two susceptible A. myosuroides populations (Fig. 1B).


Effect of herbicide resistance endowing Ile-1781-Leu and Asp-2078-Gly ACCase gene mutations on ACCase kinetics and growth traits in Lolium rigidum.

Vila-Aiub MM, Yu Q, Han H, Powles SB - J. Exp. Bot. (2015)

Extractable ACCase activity measured from partially purified enzyme extracts from the three- to four-leaf stage (A) L. rigidum and (B) A. myosuroides plants of ACCase herbicide–susceptible (SS), wild type (white bar), and homozygous resistant (RR) mutants (Ile-1781-Leu and Asp-2078-Gly). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507778&req=5

Figure 1: Extractable ACCase activity measured from partially purified enzyme extracts from the three- to four-leaf stage (A) L. rigidum and (B) A. myosuroides plants of ACCase herbicide–susceptible (SS), wild type (white bar), and homozygous resistant (RR) mutants (Ile-1781-Leu and Asp-2078-Gly). Asterisks indicate significant differences between mean values according to Dunnett’s post-hoc test using the mean ACCase herbicide-susceptible populations as control (α = 0.05). NS: not significant.
Mentions: The extractable ACCase activity was the same in L. rigidum plants homozygous for the Ile-1781-Leu mutation as in the wild-type herbicide-susceptible plants (Fig. 1A). In contrast, a significantly lower specific ACCase activity was found in plants homozygous for the Asp-2078-Gly (Fig. 1A). Plants with the Asp-2078-Gly mutation showed only 70% extractable ACCase activity (specific activity) when compared to susceptible wild-type plants. Similarly in A. myosuroides, plants homozygous for the Asp-2078-Gly mutation displayed a significantly reduced (40%) ACCase activity, whereas plants with the Ile-1781-Leu mutation showed no change in ACCase activity, relative to the two susceptible A. myosuroides populations (Fig. 1B).

Bottom Line: Both the negligible and significant growth reductions associated, respectively, with the Ile-1781-Leu and Asp-2078-Gly resistance mutations correlated with their impact on ACCase activity.Whereas the Ile-1781-Leu mutation showed no pleiotropic effects on ACCase kinetics, the Asp-2078-Gly mutation led to a significant reduction in ACCase activity.Similar effects of these two particular ACCase mutations on the ACCase activity of Alopecurus myosuroides were also confirmed.

View Article: PubMed Central - PubMed

Affiliation: Australian Herbicide Resistance Initiative (AHRI) - School of Plant Biology, University of Western Australia, WA, 6009, Australia IFEVA - CONICET - Facultad de Agronomía, Universidad de Buenos Aires (UBA), Buenos Aires, 1417, Argentina vila@ifeva.edu.ar.

No MeSH data available.


Related in: MedlinePlus