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Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus

Expression patterns in tfl1 mutant backgrounds. (A, B) Young 10-day-old tfl1 mutants showing tfl1-1 (A) and LFY (B) expression. The insert in (B) shows plants just starting to bolt and ectopically expressing LFY in the shoot. (C, D) Ten-day-old day tfl1 plants containing pLFY::TFL1 showing TFL1/tfl1-1 (C) and absence of LFY (D) expression. (E, F) Older tfl1 plants containing pLFY::TFL1 at 17 d showing TFL1/tfl1-1 (E) and LFY (F) expression. The left insert in (E) shows expression at 20 d in another line in an ap1-like structure. The right insert shows that TFL1 expression is largely limited to the shoot meristem in ap1 mutants. (G, H) At 21 d, tfl1 mutants containing pAP1::TFL1, showing TFL1/tfl1-1 (G) and AP1 (H) expression. Inserts in (G) show other examples. Corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bars=100 μm.
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Figure 6: Expression patterns in tfl1 mutant backgrounds. (A, B) Young 10-day-old tfl1 mutants showing tfl1-1 (A) and LFY (B) expression. The insert in (B) shows plants just starting to bolt and ectopically expressing LFY in the shoot. (C, D) Ten-day-old day tfl1 plants containing pLFY::TFL1 showing TFL1/tfl1-1 (C) and absence of LFY (D) expression. (E, F) Older tfl1 plants containing pLFY::TFL1 at 17 d showing TFL1/tfl1-1 (E) and LFY (F) expression. The left insert in (E) shows expression at 20 d in another line in an ap1-like structure. The right insert shows that TFL1 expression is largely limited to the shoot meristem in ap1 mutants. (G, H) At 21 d, tfl1 mutants containing pAP1::TFL1, showing TFL1/tfl1-1 (G) and AP1 (H) expression. Inserts in (G) show other examples. Corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bars=100 μm.

Mentions: The TFL1/tfl1-1 expression patterns were compared with those of LFY and AP1. Due to having common promoters, the pattern of LFY mRNA reflected the pattern of TFL1 in pLFY::TFL1 expression. In contrast, tfl1-1 mRNA reflected its own promoter and this was highest in shoot-like structures. As tfl1 mutant plants were just bolting, tfl1-1 expression was seen in the main shoot (in the centre below the dome) at the same time as LFY also became ectopically expressed there (Fig. 6A, B). In pLFY::TFL1, the TFL1/tfl1-1 RNA was also seen at bolting, but no clear expression was seen of LFY at this time, reflecting the delay in flowering (Fig. 6C, D). After bolting, pLFY::TFL1 lines had TFL1/tfl11-1 expression that partially overlapped with that of LFY (Fig. 6E, F). Ectopic LFY in the shoot meristem was weak compared with LFY in lateral meristems (Fig. 6F). Ectopic expression of TFL1/tfl1-1 in the shoot meristem, beyond the central cells, was again seen. Some structures appeared ap1-like in tissue sections, and had ectopic TFL1/tfl11-1 (Fig. 6E left insert). In contrast, in ap1 mutant plants, ap1 mutant flowers did not appear to have strong ectopic TFL1 expression (Fig. 6E right insert).


Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Expression patterns in tfl1 mutant backgrounds. (A, B) Young 10-day-old tfl1 mutants showing tfl1-1 (A) and LFY (B) expression. The insert in (B) shows plants just starting to bolt and ectopically expressing LFY in the shoot. (C, D) Ten-day-old day tfl1 plants containing pLFY::TFL1 showing TFL1/tfl1-1 (C) and absence of LFY (D) expression. (E, F) Older tfl1 plants containing pLFY::TFL1 at 17 d showing TFL1/tfl1-1 (E) and LFY (F) expression. The left insert in (E) shows expression at 20 d in another line in an ap1-like structure. The right insert shows that TFL1 expression is largely limited to the shoot meristem in ap1 mutants. (G, H) At 21 d, tfl1 mutants containing pAP1::TFL1, showing TFL1/tfl1-1 (G) and AP1 (H) expression. Inserts in (G) show other examples. Corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bars=100 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 6: Expression patterns in tfl1 mutant backgrounds. (A, B) Young 10-day-old tfl1 mutants showing tfl1-1 (A) and LFY (B) expression. The insert in (B) shows plants just starting to bolt and ectopically expressing LFY in the shoot. (C, D) Ten-day-old day tfl1 plants containing pLFY::TFL1 showing TFL1/tfl1-1 (C) and absence of LFY (D) expression. (E, F) Older tfl1 plants containing pLFY::TFL1 at 17 d showing TFL1/tfl1-1 (E) and LFY (F) expression. The left insert in (E) shows expression at 20 d in another line in an ap1-like structure. The right insert shows that TFL1 expression is largely limited to the shoot meristem in ap1 mutants. (G, H) At 21 d, tfl1 mutants containing pAP1::TFL1, showing TFL1/tfl1-1 (G) and AP1 (H) expression. Inserts in (G) show other examples. Corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bars=100 μm.
Mentions: The TFL1/tfl1-1 expression patterns were compared with those of LFY and AP1. Due to having common promoters, the pattern of LFY mRNA reflected the pattern of TFL1 in pLFY::TFL1 expression. In contrast, tfl1-1 mRNA reflected its own promoter and this was highest in shoot-like structures. As tfl1 mutant plants were just bolting, tfl1-1 expression was seen in the main shoot (in the centre below the dome) at the same time as LFY also became ectopically expressed there (Fig. 6A, B). In pLFY::TFL1, the TFL1/tfl1-1 RNA was also seen at bolting, but no clear expression was seen of LFY at this time, reflecting the delay in flowering (Fig. 6C, D). After bolting, pLFY::TFL1 lines had TFL1/tfl11-1 expression that partially overlapped with that of LFY (Fig. 6E, F). Ectopic LFY in the shoot meristem was weak compared with LFY in lateral meristems (Fig. 6F). Ectopic expression of TFL1/tfl1-1 in the shoot meristem, beyond the central cells, was again seen. Some structures appeared ap1-like in tissue sections, and had ectopic TFL1/tfl11-1 (Fig. 6E left insert). In contrast, in ap1 mutant plants, ap1 mutant flowers did not appear to have strong ectopic TFL1 expression (Fig. 6E right insert).

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus