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Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus

TFL1 and floral gene expression patterns in inflorescences in the WT background. (A, B) WT flowering shoots in the I2 phase at 21 d showing expression of TFL1 (A) or AP1 (B). Inserts in TFL1 (A) and LFY (B) at 17 d. (C, D) WT plants containing pLFY::TFL1 at 16 d showing TFL1 (C) and LFY (D) expression. Inserts show another transgenic line at 21 d. (E, F) WT plants containing pAP1::TFL1 at 21 d showing TFL1 (E) and (AP1) expression. Inserts show another line. (G–I) WT plants containing pANT::TFL1. Expression of TFL1 in a young tertiary shoot (G). Expression of TFL1 in an older secondary shoot (H) and AP1 expression (I). Shoot apical meristem (SAM), flower (F), and corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bar=100 μm.
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Figure 5: TFL1 and floral gene expression patterns in inflorescences in the WT background. (A, B) WT flowering shoots in the I2 phase at 21 d showing expression of TFL1 (A) or AP1 (B). Inserts in TFL1 (A) and LFY (B) at 17 d. (C, D) WT plants containing pLFY::TFL1 at 16 d showing TFL1 (C) and LFY (D) expression. Inserts show another transgenic line at 21 d. (E, F) WT plants containing pAP1::TFL1 at 21 d showing TFL1 (E) and (AP1) expression. Inserts show another line. (G–I) WT plants containing pANT::TFL1. Expression of TFL1 in a young tertiary shoot (G). Expression of TFL1 in an older secondary shoot (H) and AP1 expression (I). Shoot apical meristem (SAM), flower (F), and corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bar=100 μm.

Mentions: In the WT, TFL1 was expressed in the centre of the shoot meristem (and weakly in inflorescence stems), but not in lateral primordia or floral meristems (Fig. 5A). In a complementary manner, LFY and AP1 were not expressed in the shoot meristem of the WT, but only in the lateral meristems of I2, the floral meristems (Fig. 5B).


Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

TFL1 and floral gene expression patterns in inflorescences in the WT background. (A, B) WT flowering shoots in the I2 phase at 21 d showing expression of TFL1 (A) or AP1 (B). Inserts in TFL1 (A) and LFY (B) at 17 d. (C, D) WT plants containing pLFY::TFL1 at 16 d showing TFL1 (C) and LFY (D) expression. Inserts show another transgenic line at 21 d. (E, F) WT plants containing pAP1::TFL1 at 21 d showing TFL1 (E) and (AP1) expression. Inserts show another line. (G–I) WT plants containing pANT::TFL1. Expression of TFL1 in a young tertiary shoot (G). Expression of TFL1 in an older secondary shoot (H) and AP1 expression (I). Shoot apical meristem (SAM), flower (F), and corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bar=100 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 5: TFL1 and floral gene expression patterns in inflorescences in the WT background. (A, B) WT flowering shoots in the I2 phase at 21 d showing expression of TFL1 (A) or AP1 (B). Inserts in TFL1 (A) and LFY (B) at 17 d. (C, D) WT plants containing pLFY::TFL1 at 16 d showing TFL1 (C) and LFY (D) expression. Inserts show another transgenic line at 21 d. (E, F) WT plants containing pAP1::TFL1 at 21 d showing TFL1 (E) and (AP1) expression. Inserts show another line. (G–I) WT plants containing pANT::TFL1. Expression of TFL1 in a young tertiary shoot (G). Expression of TFL1 in an older secondary shoot (H) and AP1 expression (I). Shoot apical meristem (SAM), flower (F), and corresponding mRNA signals seen as a purple stain on pale blue/white tissue background. Scale bar=100 μm.
Mentions: In the WT, TFL1 was expressed in the centre of the shoot meristem (and weakly in inflorescence stems), but not in lateral primordia or floral meristems (Fig. 5A). In a complementary manner, LFY and AP1 were not expressed in the shoot meristem of the WT, but only in the lateral meristems of I2, the floral meristems (Fig. 5B).

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus