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Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus

Early TFL1/tfl1-1 expression patterns in the tfl1-1 background. (A–D) TFL1/tfl1-1 expression at 6 d in tfl1-1 (A) or tfl1-1 containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). The shoot apical meristem is generating rosette leaves (RL). (E–H) TFL1/tfl1-1 expression at 12–14 d in the I1–I2 phase of tfl1-1 (E) or tfl11-1 containing pANT::TFL1 (F), pLFY::TFL1 (G), or pAP1::TFL1 (H). In tfl1, the SAM has already converted to a terminal flower (TF) while the other lines have TFL1/tfl1-1 mRNA in the shoot apical meristems but are still not terminating at this stage. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.
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Figure 4: Early TFL1/tfl1-1 expression patterns in the tfl1-1 background. (A–D) TFL1/tfl1-1 expression at 6 d in tfl1-1 (A) or tfl1-1 containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). The shoot apical meristem is generating rosette leaves (RL). (E–H) TFL1/tfl1-1 expression at 12–14 d in the I1–I2 phase of tfl1-1 (E) or tfl11-1 containing pANT::TFL1 (F), pLFY::TFL1 (G), or pAP1::TFL1 (H). In tfl1, the SAM has already converted to a terminal flower (TF) while the other lines have TFL1/tfl1-1 mRNA in the shoot apical meristems but are still not terminating at this stage. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.

Mentions: In the tfl1 mutant background, no endogenous mutant tfl1-1 mRNA was seen at the early phase (Fig. 4A). Similarly, no transgenic TFL1 or tfl1-1 signal (together referred to as TFL1/tfl1-1) was seen at early time points, suggesting that it was below the detection limit (Fig. 4A–D). Thus, despite undetectable expression, all promoter::TFL1 lines in tfl1 had V phases restored to WT. Therefore, tfl1 may be easily complemented by different promoters, but the length of the V phase may be generally robust due to many flowering pathways controlling the ‘veg’ character of this growth phase (Prusinkiewicz et al., 2007).


Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Early TFL1/tfl1-1 expression patterns in the tfl1-1 background. (A–D) TFL1/tfl1-1 expression at 6 d in tfl1-1 (A) or tfl1-1 containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). The shoot apical meristem is generating rosette leaves (RL). (E–H) TFL1/tfl1-1 expression at 12–14 d in the I1–I2 phase of tfl1-1 (E) or tfl11-1 containing pANT::TFL1 (F), pLFY::TFL1 (G), or pAP1::TFL1 (H). In tfl1, the SAM has already converted to a terminal flower (TF) while the other lines have TFL1/tfl1-1 mRNA in the shoot apical meristems but are still not terminating at this stage. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507777&req=5

Figure 4: Early TFL1/tfl1-1 expression patterns in the tfl1-1 background. (A–D) TFL1/tfl1-1 expression at 6 d in tfl1-1 (A) or tfl1-1 containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). The shoot apical meristem is generating rosette leaves (RL). (E–H) TFL1/tfl1-1 expression at 12–14 d in the I1–I2 phase of tfl1-1 (E) or tfl11-1 containing pANT::TFL1 (F), pLFY::TFL1 (G), or pAP1::TFL1 (H). In tfl1, the SAM has already converted to a terminal flower (TF) while the other lines have TFL1/tfl1-1 mRNA in the shoot apical meristems but are still not terminating at this stage. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.
Mentions: In the tfl1 mutant background, no endogenous mutant tfl1-1 mRNA was seen at the early phase (Fig. 4A). Similarly, no transgenic TFL1 or tfl1-1 signal (together referred to as TFL1/tfl1-1) was seen at early time points, suggesting that it was below the detection limit (Fig. 4A–D). Thus, despite undetectable expression, all promoter::TFL1 lines in tfl1 had V phases restored to WT. Therefore, tfl1 may be easily complemented by different promoters, but the length of the V phase may be generally robust due to many flowering pathways controlling the ‘veg’ character of this growth phase (Prusinkiewicz et al., 2007).

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus