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Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus

Early TFL1 expression patterns in the WT background. (A–E) TFL1 expression in the vegetative phase (6-day-old plants) of the WT (A) or the WT containing pANT::TFL1 (B), pLFY::TFL1 (C), pAP1::TFL1 (D), or p35S::TFL1 (E). For example, in (A), the shoot apical meristem (SAM) and rosette leaves (RL) generated by this meristem are indicated. (F–J) TFL1 expression in early to late I1 phase (10- to 12-day-old plants) of the WT (F) or the WT containing pANT::TFL1 (G), pLFY::TFL1 (H), pAP1::TFL1 (I), or p35S::TFL1 (J). Examples of inflorescence SAM, axillary meristems (AxM) in axils of cauline leaves (CL) and floral meristems (FM) are highlighted. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.
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Figure 3: Early TFL1 expression patterns in the WT background. (A–E) TFL1 expression in the vegetative phase (6-day-old plants) of the WT (A) or the WT containing pANT::TFL1 (B), pLFY::TFL1 (C), pAP1::TFL1 (D), or p35S::TFL1 (E). For example, in (A), the shoot apical meristem (SAM) and rosette leaves (RL) generated by this meristem are indicated. (F–J) TFL1 expression in early to late I1 phase (10- to 12-day-old plants) of the WT (F) or the WT containing pANT::TFL1 (G), pLFY::TFL1 (H), pAP1::TFL1 (I), or p35S::TFL1 (J). Examples of inflorescence SAM, axillary meristems (AxM) in axils of cauline leaves (CL) and floral meristems (FM) are highlighted. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.

Mentions: The expression patterns of TFL1 were analysed in the different lines by RNA in situ hybridization to see how they related to the plant phenotypes. In the vegetative phase of all WT lines, no early endogenous or transgenic TFL1 expression was clearly seen, except for general, constitutive p35S::TFL1 (Fig. 3A–E). Thus the lack of any TFL1 effect on the vegetative phase was most probably simply a lack of detectable expression. This may reflect the use of promoter fragments, sensitivity of detection, or even mRNA stability at early stages.


Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Early TFL1 expression patterns in the WT background. (A–E) TFL1 expression in the vegetative phase (6-day-old plants) of the WT (A) or the WT containing pANT::TFL1 (B), pLFY::TFL1 (C), pAP1::TFL1 (D), or p35S::TFL1 (E). For example, in (A), the shoot apical meristem (SAM) and rosette leaves (RL) generated by this meristem are indicated. (F–J) TFL1 expression in early to late I1 phase (10- to 12-day-old plants) of the WT (F) or the WT containing pANT::TFL1 (G), pLFY::TFL1 (H), pAP1::TFL1 (I), or p35S::TFL1 (J). Examples of inflorescence SAM, axillary meristems (AxM) in axils of cauline leaves (CL) and floral meristems (FM) are highlighted. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507777&req=5

Figure 3: Early TFL1 expression patterns in the WT background. (A–E) TFL1 expression in the vegetative phase (6-day-old plants) of the WT (A) or the WT containing pANT::TFL1 (B), pLFY::TFL1 (C), pAP1::TFL1 (D), or p35S::TFL1 (E). For example, in (A), the shoot apical meristem (SAM) and rosette leaves (RL) generated by this meristem are indicated. (F–J) TFL1 expression in early to late I1 phase (10- to 12-day-old plants) of the WT (F) or the WT containing pANT::TFL1 (G), pLFY::TFL1 (H), pAP1::TFL1 (I), or p35S::TFL1 (J). Examples of inflorescence SAM, axillary meristems (AxM) in axils of cauline leaves (CL) and floral meristems (FM) are highlighted. All images were obtained with the same probes and signals developed for the same time. Signal is seen as a purple stain on a pale/pink background. Scale bar=100 μm.
Mentions: The expression patterns of TFL1 were analysed in the different lines by RNA in situ hybridization to see how they related to the plant phenotypes. In the vegetative phase of all WT lines, no early endogenous or transgenic TFL1 expression was clearly seen, except for general, constitutive p35S::TFL1 (Fig. 3A–E). Thus the lack of any TFL1 effect on the vegetative phase was most probably simply a lack of detectable expression. This may reflect the use of promoter fragments, sensitivity of detection, or even mRNA stability at early stages.

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus