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Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Plant architectures due to TFL1 expression. (A–D) Mature plants of Arabidopsis WT (A) or WT containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). In (A), the WT phases V, I1, and I2 are indicated. (E, F) Young tfl1-1 mutant plants already bolted with terminal flowers (E) compared with tfl1 containing pAP1::TFL1 at the same age of 16 d (F). (G–I) Mature plants showing tfl1-1 (G) or tfl1-1 containing pLFY::TFL1 (H) or pAP1::TFL1 (I). the insert in (H) shows that these plants eventually make normal flowers and terminate. Insert in (I) shows CLs with axillary ap1-like structures. Scale bars=1cm.
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Figure 2: Plant architectures due to TFL1 expression. (A–D) Mature plants of Arabidopsis WT (A) or WT containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). In (A), the WT phases V, I1, and I2 are indicated. (E, F) Young tfl1-1 mutant plants already bolted with terminal flowers (E) compared with tfl1 containing pAP1::TFL1 at the same age of 16 d (F). (G–I) Mature plants showing tfl1-1 (G) or tfl1-1 containing pLFY::TFL1 (H) or pAP1::TFL1 (I). the insert in (H) shows that these plants eventually make normal flowers and terminate. Insert in (I) shows CLs with axillary ap1-like structures. Scale bars=1cm.

Mentions: Analysis of pANT::TFL1 or pLFY::TFL1 in the tfl1 mutant showed that the V phase was extended compared with tfl1 (Fig. 1). The tfl1 mutant had a shorter V phase compared with transformants (Fig. 2E, F). After ~16–20 d, the tfl1 mutant had already flowered and made seed pods (siliques). At this time point, tfl1 lines carrying pLFY::TFL1 or pAP1::TFL1 had just started to bolt and were making flowers that had not yet matured. The common effect in all lines was to restore the V phase to WT (Fig. 1). Interestingly, pAP1::TFL1 could also restore the V phase to WT in the strongest examples, and weaker lines always made significantly more RLs than tfl1. Unlike p35S, all other promoter lines made WT numbers of RLs, not more.


Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Plant architectures due to TFL1 expression. (A–D) Mature plants of Arabidopsis WT (A) or WT containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). In (A), the WT phases V, I1, and I2 are indicated. (E, F) Young tfl1-1 mutant plants already bolted with terminal flowers (E) compared with tfl1 containing pAP1::TFL1 at the same age of 16 d (F). (G–I) Mature plants showing tfl1-1 (G) or tfl1-1 containing pLFY::TFL1 (H) or pAP1::TFL1 (I). the insert in (H) shows that these plants eventually make normal flowers and terminate. Insert in (I) shows CLs with axillary ap1-like structures. Scale bars=1cm.
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Related In: Results  -  Collection

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Figure 2: Plant architectures due to TFL1 expression. (A–D) Mature plants of Arabidopsis WT (A) or WT containing pANT::TFL1 (B), pLFY::TFL1 (C), or pAP1::TFL1 (D). In (A), the WT phases V, I1, and I2 are indicated. (E, F) Young tfl1-1 mutant plants already bolted with terminal flowers (E) compared with tfl1 containing pAP1::TFL1 at the same age of 16 d (F). (G–I) Mature plants showing tfl1-1 (G) or tfl1-1 containing pLFY::TFL1 (H) or pAP1::TFL1 (I). the insert in (H) shows that these plants eventually make normal flowers and terminate. Insert in (I) shows CLs with axillary ap1-like structures. Scale bars=1cm.
Mentions: Analysis of pANT::TFL1 or pLFY::TFL1 in the tfl1 mutant showed that the V phase was extended compared with tfl1 (Fig. 1). The tfl1 mutant had a shorter V phase compared with transformants (Fig. 2E, F). After ~16–20 d, the tfl1 mutant had already flowered and made seed pods (siliques). At this time point, tfl1 lines carrying pLFY::TFL1 or pAP1::TFL1 had just started to bolt and were making flowers that had not yet matured. The common effect in all lines was to restore the V phase to WT (Fig. 1). Interestingly, pAP1::TFL1 could also restore the V phase to WT in the strongest examples, and weaker lines always made significantly more RLs than tfl1. Unlike p35S, all other promoter lines made WT numbers of RLs, not more.

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.