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Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus

Ectopic TFL1 affects plant organ numbers. The number of rosette leaves (RLs), cauline leaves (CLs), I1* structures (shoots without subtending CLs or ap1-like structures), and flowers (Fs) made by the main shoot were recorded for wild-type (WT) Arabidopsis or tfl1-1 mutants containing pANT::TFL1, pLFY::TFL1, or pAP1::TFL1. WT plants containing p35S::TFL1 and ap1-12 mutants were also analysed. Numbers represent the average of 23–54 plants with standard deviations as shown. The solid black bars in (F) in the tfl1 background represent termination of the main shoot by conversion to a flower.
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Figure 1: Ectopic TFL1 affects plant organ numbers. The number of rosette leaves (RLs), cauline leaves (CLs), I1* structures (shoots without subtending CLs or ap1-like structures), and flowers (Fs) made by the main shoot were recorded for wild-type (WT) Arabidopsis or tfl1-1 mutants containing pANT::TFL1, pLFY::TFL1, or pAP1::TFL1. WT plants containing p35S::TFL1 and ap1-12 mutants were also analysed. Numbers represent the average of 23–54 plants with standard deviations as shown. The solid black bars in (F) in the tfl1 background represent termination of the main shoot by conversion to a flower.

Mentions: WT plants made ~11 RLs in long days (Fig. 1). No significant changes in leaf number were observed in any lines carrying any of the three constructs (Fig. 1). The range of leaf numbers was greater in plants carrying pANT::TFL1 (9–16 leaves compared with 10–13 for the WT), but the averages were not statistically significant. This variability for pANT::TFL1 was seen in different experiments. In contrast, plants carrying p35S::TFL1 (which is strongly expressed in all tissues, including primordia and the shoot meristem) had an extension of the vegetative rosette (V) phase to 19 RLs, as previously shown (Fig. 1; Ratcliffe et al., 1998).


Changing the spatial pattern of TFL1 expression reveals its key role in the shoot meristem in controlling Arabidopsis flowering architecture.

Baumann K, Venail J, Berbel A, Domenech MJ, Money T, Conti L, Hanzawa Y, Madueno F, Bradley D - J. Exp. Bot. (2015)

Ectopic TFL1 affects plant organ numbers. The number of rosette leaves (RLs), cauline leaves (CLs), I1* structures (shoots without subtending CLs or ap1-like structures), and flowers (Fs) made by the main shoot were recorded for wild-type (WT) Arabidopsis or tfl1-1 mutants containing pANT::TFL1, pLFY::TFL1, or pAP1::TFL1. WT plants containing p35S::TFL1 and ap1-12 mutants were also analysed. Numbers represent the average of 23–54 plants with standard deviations as shown. The solid black bars in (F) in the tfl1 background represent termination of the main shoot by conversion to a flower.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507777&req=5

Figure 1: Ectopic TFL1 affects plant organ numbers. The number of rosette leaves (RLs), cauline leaves (CLs), I1* structures (shoots without subtending CLs or ap1-like structures), and flowers (Fs) made by the main shoot were recorded for wild-type (WT) Arabidopsis or tfl1-1 mutants containing pANT::TFL1, pLFY::TFL1, or pAP1::TFL1. WT plants containing p35S::TFL1 and ap1-12 mutants were also analysed. Numbers represent the average of 23–54 plants with standard deviations as shown. The solid black bars in (F) in the tfl1 background represent termination of the main shoot by conversion to a flower.
Mentions: WT plants made ~11 RLs in long days (Fig. 1). No significant changes in leaf number were observed in any lines carrying any of the three constructs (Fig. 1). The range of leaf numbers was greater in plants carrying pANT::TFL1 (9–16 leaves compared with 10–13 for the WT), but the averages were not statistically significant. This variability for pANT::TFL1 was seen in different experiments. In contrast, plants carrying p35S::TFL1 (which is strongly expressed in all tissues, including primordia and the shoot meristem) had an extension of the vegetative rosette (V) phase to 19 RLs, as previously shown (Fig. 1; Ratcliffe et al., 1998).

Bottom Line: These data show how the timing and spatial pattern of TFL1 expression affect overall plant architecture.However, the data show that while TFL1 and floral genes can both act and compete in the same meristem, it appears that the main shoot meristem is more sensitive to TFL1 rather than floral genes.This spatial analysis therefore reveals how a difference in response helps maintain the 'veg' state of the shoot meristem.

View Article: PubMed Central - PubMed

Affiliation: John Innes Centre, Colney, Norwich NR4 7UH, UK.

No MeSH data available.


Related in: MedlinePlus