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SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Na+ accumulation in AtPP2-B11 transgenic and wild-type plants exposed to salt stress. (A) Na+ accumulation in the cotyledons of 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water as control or with 200mM NaCl for 6h. Na+ was visualized using CoroNa Green dye by laser-scanning confocal microscope. (B) Na+ content in 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water (control) or 200mM NaCl for 6h. (C) Real-time RT-PCR analysis of genes involved in Na+ homeostasis. Total RNA from 2-week-old AtPP2-B11 transgenic and wild-type seedlings was extracted and analysed by real-time RT-PCR. The graphs indicate the fold induction of HKT1, NHX1, SOS1, SOS2, and SOS3 in response to salt stress compared with the control (CK). Mean values from three independent technical replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (This figure is available in colour at JXB online.)
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Figure 8: Na+ accumulation in AtPP2-B11 transgenic and wild-type plants exposed to salt stress. (A) Na+ accumulation in the cotyledons of 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water as control or with 200mM NaCl for 6h. Na+ was visualized using CoroNa Green dye by laser-scanning confocal microscope. (B) Na+ content in 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water (control) or 200mM NaCl for 6h. (C) Real-time RT-PCR analysis of genes involved in Na+ homeostasis. Total RNA from 2-week-old AtPP2-B11 transgenic and wild-type seedlings was extracted and analysed by real-time RT-PCR. The graphs indicate the fold induction of HKT1, NHX1, SOS1, SOS2, and SOS3 in response to salt stress compared with the control (CK). Mean values from three independent technical replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (This figure is available in colour at JXB online.)

Mentions: To understand the physiological role of AtPP2-B11 overexpression in enhancing tolerance to salt stress, the accumulation of Na+ was visualized in 2-week-old plants by confocal microscopy using CoroNa Green dye (a green fluorescent indicator of Na+). No differences were observed in the Na+ fluorescence of the transgenic lines OE10, OE11, and R-5 and the wild-type plants under normal conditions. When treated with 200mM NaCl for 6h, the fluorescence of OE10 and OE11 was barely detectable, while intense fluorescence could clearly be observed in leaves of the wild-type and R-5 plants (Fig. 8A). To confirm the above results, Na+ levels were also measured using atomic absorption. OE10 and OE11 contained lower concentrations of Na+ than the R-5 line and wild-type plants, which was consistent with the results of Na+ fluorescence analysis (Fig. 8B).


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Na+ accumulation in AtPP2-B11 transgenic and wild-type plants exposed to salt stress. (A) Na+ accumulation in the cotyledons of 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water as control or with 200mM NaCl for 6h. Na+ was visualized using CoroNa Green dye by laser-scanning confocal microscope. (B) Na+ content in 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water (control) or 200mM NaCl for 6h. (C) Real-time RT-PCR analysis of genes involved in Na+ homeostasis. Total RNA from 2-week-old AtPP2-B11 transgenic and wild-type seedlings was extracted and analysed by real-time RT-PCR. The graphs indicate the fold induction of HKT1, NHX1, SOS1, SOS2, and SOS3 in response to salt stress compared with the control (CK). Mean values from three independent technical replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (This figure is available in colour at JXB online.)
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Figure 8: Na+ accumulation in AtPP2-B11 transgenic and wild-type plants exposed to salt stress. (A) Na+ accumulation in the cotyledons of 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water as control or with 200mM NaCl for 6h. Na+ was visualized using CoroNa Green dye by laser-scanning confocal microscope. (B) Na+ content in 2-week-old AtPP2-B11 transgenic and wild-type seedlings treated with water (control) or 200mM NaCl for 6h. (C) Real-time RT-PCR analysis of genes involved in Na+ homeostasis. Total RNA from 2-week-old AtPP2-B11 transgenic and wild-type seedlings was extracted and analysed by real-time RT-PCR. The graphs indicate the fold induction of HKT1, NHX1, SOS1, SOS2, and SOS3 in response to salt stress compared with the control (CK). Mean values from three independent technical replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (This figure is available in colour at JXB online.)
Mentions: To understand the physiological role of AtPP2-B11 overexpression in enhancing tolerance to salt stress, the accumulation of Na+ was visualized in 2-week-old plants by confocal microscopy using CoroNa Green dye (a green fluorescent indicator of Na+). No differences were observed in the Na+ fluorescence of the transgenic lines OE10, OE11, and R-5 and the wild-type plants under normal conditions. When treated with 200mM NaCl for 6h, the fluorescence of OE10 and OE11 was barely detectable, while intense fluorescence could clearly be observed in leaves of the wild-type and R-5 plants (Fig. 8A). To confirm the above results, Na+ levels were also measured using atomic absorption. OE10 and OE11 contained lower concentrations of Na+ than the R-5 line and wild-type plants, which was consistent with the results of Na+ fluorescence analysis (Fig. 8B).

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.