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SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


AtPP2-B11 altered cellular redox homeostasis in Arabidopsis. (A) ROS histological staining analysis of AtPP2-B11 transgenic plants and wild-type plants. The H2O2 and O2– accumulation in seedlings of OE10, OE11, R-5 and wild-type plants were analysed by DAB and NBT staining, respectively. (B) GSH/GSSG ratio in 2-week-old OE10, OE11, R-5 and wild-type seedlings under normal (CK) or salt stress (NaCl) conditions. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT 2 d after germination. (D) Salt responses of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (E) Root length of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (This figure is available in colour at JXB online.)
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Figure 7: AtPP2-B11 altered cellular redox homeostasis in Arabidopsis. (A) ROS histological staining analysis of AtPP2-B11 transgenic plants and wild-type plants. The H2O2 and O2– accumulation in seedlings of OE10, OE11, R-5 and wild-type plants were analysed by DAB and NBT staining, respectively. (B) GSH/GSSG ratio in 2-week-old OE10, OE11, R-5 and wild-type seedlings under normal (CK) or salt stress (NaCl) conditions. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT 2 d after germination. (D) Salt responses of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (E) Root length of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (This figure is available in colour at JXB online.)

Mentions: iTRAQ analysis revealed that many redox proteins were upregulated in OE11 plants under salt stress (Supplementary Table S3). Considering that AtPP2-B11 overexpressing lines also enhanced the expressions of annexins, and that annexins act as a molecular link between abiotic stress and ROS signalling, we asked whether the redox homeostasis of AtPP2-B11 transgenic lines was also altered. In order to verify our hypothesis, ROS accumulation in AtPP2-B11 transgenic Arabidopsis was analysed. The results of DAB staining showed that H2O2 levels were not significantly different in these seedlings under normal conditions (data not shown). Notably, the OE10 and OE11 seedlings accumulated less H2O2 compared with the wild-type plants when treated with 200mM NaCl, whereas the accumulation of H2O2 in the R-5 seedlings was significantly elevated (Fig. 7A). In addition, we also determined O2– levels using NBT staining. The results were similar to those of DAB staining: salinity caused an increase in NBT staining in the R-5 plants, especially in the cotyledons (Fig. 7A). Moreover, the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) was measured, and the results showed that OE10 and OE11 had higher ratios of GSH:GSSG compared with wild-type plants in salt stress conditions, which was consistent with the results of DAB and NBT staining analysis (Fig. 7B).


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

AtPP2-B11 altered cellular redox homeostasis in Arabidopsis. (A) ROS histological staining analysis of AtPP2-B11 transgenic plants and wild-type plants. The H2O2 and O2– accumulation in seedlings of OE10, OE11, R-5 and wild-type plants were analysed by DAB and NBT staining, respectively. (B) GSH/GSSG ratio in 2-week-old OE10, OE11, R-5 and wild-type seedlings under normal (CK) or salt stress (NaCl) conditions. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT 2 d after germination. (D) Salt responses of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (E) Root length of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (This figure is available in colour at JXB online.)
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Figure 7: AtPP2-B11 altered cellular redox homeostasis in Arabidopsis. (A) ROS histological staining analysis of AtPP2-B11 transgenic plants and wild-type plants. The H2O2 and O2– accumulation in seedlings of OE10, OE11, R-5 and wild-type plants were analysed by DAB and NBT staining, respectively. (B) GSH/GSSG ratio in 2-week-old OE10, OE11, R-5 and wild-type seedlings under normal (CK) or salt stress (NaCl) conditions. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT 2 d after germination. (D) Salt responses of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (E) Root length of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 200mM NaCl or 200mM NaCl plus 600 µM GSH or 300 µM DTT grown for 2 weeks. (This figure is available in colour at JXB online.)
Mentions: iTRAQ analysis revealed that many redox proteins were upregulated in OE11 plants under salt stress (Supplementary Table S3). Considering that AtPP2-B11 overexpressing lines also enhanced the expressions of annexins, and that annexins act as a molecular link between abiotic stress and ROS signalling, we asked whether the redox homeostasis of AtPP2-B11 transgenic lines was also altered. In order to verify our hypothesis, ROS accumulation in AtPP2-B11 transgenic Arabidopsis was analysed. The results of DAB staining showed that H2O2 levels were not significantly different in these seedlings under normal conditions (data not shown). Notably, the OE10 and OE11 seedlings accumulated less H2O2 compared with the wild-type plants when treated with 200mM NaCl, whereas the accumulation of H2O2 in the R-5 seedlings was significantly elevated (Fig. 7A). In addition, we also determined O2– levels using NBT staining. The results were similar to those of DAB staining: salinity caused an increase in NBT staining in the R-5 plants, especially in the cotyledons (Fig. 7A). Moreover, the ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) was measured, and the results showed that OE10 and OE11 had higher ratios of GSH:GSSG compared with wild-type plants in salt stress conditions, which was consistent with the results of DAB and NBT staining analysis (Fig. 7B).

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.