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SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)
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Figure 5: GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)

Mentions: To elucidate the molecular mechanism of AtPP2-B11 in response to salt stress in plants, we compared the protein profile changes between OE11 and wild-type plants using the iTRAQ technique. Since the root length and post-germination growth of 2-week-old AtPP2-B11 overexpressing plants all exhibited more tolerance to salt stress than wild-type plants, total protein of 2-week-old OE11 and wild-type plants under salt stress for 6h was extracted using a urea/CHAPS extraction buffer, digested in solution, labelled with iTRAQ reagents, and quantitatively identified by LTQ-Orbitrap XL hybrid MS scans (Supplementary Fig. S2, available at JXB online). A total of 5881 proteins were identified in the two experiments and 4311 proteins were quantitated, covering a wide range of metabolic and signalling pathways (Supplementary Table S1, available at JXB online). Compared with the wild-type plants, various proteins in the OE11 plants exhibited significant changes. At a threshold of 2-fold change, 275 and 19 proteins were up- and downregulated, while with a threshold of 1.5-fold change, a total of 635 and 167 proteins were up- and downregulated, respectively (Fig. 5A). Interestingly, the number of upregulated proteins was much higher than that of downregulated ones, indicating that AtPP2-B11 probably play positive roles in regulating other proteins under salt stress conditions.


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4507775&req=5

Figure 5: GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)
Mentions: To elucidate the molecular mechanism of AtPP2-B11 in response to salt stress in plants, we compared the protein profile changes between OE11 and wild-type plants using the iTRAQ technique. Since the root length and post-germination growth of 2-week-old AtPP2-B11 overexpressing plants all exhibited more tolerance to salt stress than wild-type plants, total protein of 2-week-old OE11 and wild-type plants under salt stress for 6h was extracted using a urea/CHAPS extraction buffer, digested in solution, labelled with iTRAQ reagents, and quantitatively identified by LTQ-Orbitrap XL hybrid MS scans (Supplementary Fig. S2, available at JXB online). A total of 5881 proteins were identified in the two experiments and 4311 proteins were quantitated, covering a wide range of metabolic and signalling pathways (Supplementary Table S1, available at JXB online). Compared with the wild-type plants, various proteins in the OE11 plants exhibited significant changes. At a threshold of 2-fold change, 275 and 19 proteins were up- and downregulated, while with a threshold of 1.5-fold change, a total of 635 and 167 proteins were up- and downregulated, respectively (Fig. 5A). Interestingly, the number of upregulated proteins was much higher than that of downregulated ones, indicating that AtPP2-B11 probably play positive roles in regulating other proteins under salt stress conditions.

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.