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SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: AtPP2-B11 could upregulate the expression of annexin1 (AnnAt1) and function as a molecular link between salt stress and reactive oxygen species accumulation in Arabidopsis.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Related in: MedlinePlus

GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)
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Figure 5: GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)

Mentions: To elucidate the molecular mechanism of AtPP2-B11 in response to salt stress in plants, we compared the protein profile changes between OE11 and wild-type plants using the iTRAQ technique. Since the root length and post-germination growth of 2-week-old AtPP2-B11 overexpressing plants all exhibited more tolerance to salt stress than wild-type plants, total protein of 2-week-old OE11 and wild-type plants under salt stress for 6h was extracted using a urea/CHAPS extraction buffer, digested in solution, labelled with iTRAQ reagents, and quantitatively identified by LTQ-Orbitrap XL hybrid MS scans (Supplementary Fig. S2, available at JXB online). A total of 5881 proteins were identified in the two experiments and 4311 proteins were quantitated, covering a wide range of metabolic and signalling pathways (Supplementary Table S1, available at JXB online). Compared with the wild-type plants, various proteins in the OE11 plants exhibited significant changes. At a threshold of 2-fold change, 275 and 19 proteins were up- and downregulated, while with a threshold of 1.5-fold change, a total of 635 and 167 proteins were up- and downregulated, respectively (Fig. 5A). Interestingly, the number of upregulated proteins was much higher than that of downregulated ones, indicating that AtPP2-B11 probably play positive roles in regulating other proteins under salt stress conditions.


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4507775&req=5

Figure 5: GO analysis of proteins upregulated by twofold in the OE11 line compared with those in wild-type plants under salt stress conditions. (A). Numbers of differentially expressed proteins of the OE11 line compared with those of wild-type plants. The ratios were >2, >1.5, <1/1.5 and <1/2. (B). ‘Biological process’ analysis of the twofold upregulated proteins. (C). ‘Molecular function’ analysis of the twofold upregulated proteins. (This figure is available in colour at JXB online.)
Mentions: To elucidate the molecular mechanism of AtPP2-B11 in response to salt stress in plants, we compared the protein profile changes between OE11 and wild-type plants using the iTRAQ technique. Since the root length and post-germination growth of 2-week-old AtPP2-B11 overexpressing plants all exhibited more tolerance to salt stress than wild-type plants, total protein of 2-week-old OE11 and wild-type plants under salt stress for 6h was extracted using a urea/CHAPS extraction buffer, digested in solution, labelled with iTRAQ reagents, and quantitatively identified by LTQ-Orbitrap XL hybrid MS scans (Supplementary Fig. S2, available at JXB online). A total of 5881 proteins were identified in the two experiments and 4311 proteins were quantitated, covering a wide range of metabolic and signalling pathways (Supplementary Table S1, available at JXB online). Compared with the wild-type plants, various proteins in the OE11 plants exhibited significant changes. At a threshold of 2-fold change, 275 and 19 proteins were up- and downregulated, while with a threshold of 1.5-fold change, a total of 635 and 167 proteins were up- and downregulated, respectively (Fig. 5A). Interestingly, the number of upregulated proteins was much higher than that of downregulated ones, indicating that AtPP2-B11 probably play positive roles in regulating other proteins under salt stress conditions.

Bottom Line: AtPP2-B11 could upregulate the expression of annexin1 (AnnAt1) and function as a molecular link between salt stress and reactive oxygen species accumulation in Arabidopsis.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Related in: MedlinePlus