Limits...
SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress at the adult stage. (A) Photographs of AtPP2-B11 transgenic and wild-type plants after watering with 200mM NaCl. The transgenic and wild-type plants were transferred to soil grown for 4 weeks under normal growth conditions and then watered with 200mM NaCl for 20 d. (B) Survival rates of AtPP2-B11 transgenic and wild-type plants. Survival rates were determined after 3 d of rewatering. Fifty plants were tested in each experiment. (C) MDA contents of AtPP2-B11 transgenic and wild-type plants. Four-week-old AtPP2-B11 transgenic and wild-type plants were treated with 200mM NaCl for 6h, and water replaced the NaCl as a control. (D, E). Chlorophyll a (D) and b (E) levels of AtPP2-B11 transgenic and wild-type plants before and after watering with 200mM NaCl for 10 d at the adult stage. Data showed the mean±SD of at least three replicates. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507775&req=5

Figure 4: Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress at the adult stage. (A) Photographs of AtPP2-B11 transgenic and wild-type plants after watering with 200mM NaCl. The transgenic and wild-type plants were transferred to soil grown for 4 weeks under normal growth conditions and then watered with 200mM NaCl for 20 d. (B) Survival rates of AtPP2-B11 transgenic and wild-type plants. Survival rates were determined after 3 d of rewatering. Fifty plants were tested in each experiment. (C) MDA contents of AtPP2-B11 transgenic and wild-type plants. Four-week-old AtPP2-B11 transgenic and wild-type plants were treated with 200mM NaCl for 6h, and water replaced the NaCl as a control. (D, E). Chlorophyll a (D) and b (E) levels of AtPP2-B11 transgenic and wild-type plants before and after watering with 200mM NaCl for 10 d at the adult stage. Data showed the mean±SD of at least three replicates. (This figure is available in colour at JXB online.)

Mentions: To investigate further whether AtPP2-B11 functions at the adult stage, adult plants of the OE10, OE11, and R-5 lines and wild-type plants were tested for their response to high salinity. The seeds of these four genotypes were plated on ½ MS for 7 d and then transferred to soil growth for 4 weeks. Healthy and uniform transgenic and wild-type plants were selected for further treatment with or without 200mM NaCl for 20 d. Under normal growth conditions, the transgenic lines and wild-type plants showed no significant morphological or developmental abnormalities. However, when subjected to salt stress, the OE10 and OE11 lines had much greener and truer leaves, whereas a higher percentage of pale seedlings was observed in R-5 plants (Fig. 4A). Three days after rewatering, the survival ratios of the OE10 and OE11 lines were 51 and 60%, while the survival ratio of R-5 and wild-type plants were only 23 and 35%, respectively (Fig. 4B). The content of malondialdehyde (MDA) was also measured in AtPP2-B11 transgenic plants and wild-type plants, which could represent the oxidative level of membrane lipid. Although the accumulation of MDA in all four genotypes was significantly elevated under salt stress, the OE10 and OE11 lines had lower MDA levels than the wild-type and R-5 plants (Fig. 4C). In addition, the levels of chlorophyll of transgenic Arabidopsis and wild-type plants were similar under normal growth conditions. However, under salt growth conditions, the OE10 and OE11 lines had higher levels of chlorophyll a and chlorophyll b than the wild-type and R-5 plants (Fig. 4D, E). These results indicated that AtPP2-B11 acts as a positive regulator in response to salt at both the seedling and adult stages.


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress at the adult stage. (A) Photographs of AtPP2-B11 transgenic and wild-type plants after watering with 200mM NaCl. The transgenic and wild-type plants were transferred to soil grown for 4 weeks under normal growth conditions and then watered with 200mM NaCl for 20 d. (B) Survival rates of AtPP2-B11 transgenic and wild-type plants. Survival rates were determined after 3 d of rewatering. Fifty plants were tested in each experiment. (C) MDA contents of AtPP2-B11 transgenic and wild-type plants. Four-week-old AtPP2-B11 transgenic and wild-type plants were treated with 200mM NaCl for 6h, and water replaced the NaCl as a control. (D, E). Chlorophyll a (D) and b (E) levels of AtPP2-B11 transgenic and wild-type plants before and after watering with 200mM NaCl for 10 d at the adult stage. Data showed the mean±SD of at least three replicates. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507775&req=5

Figure 4: Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress at the adult stage. (A) Photographs of AtPP2-B11 transgenic and wild-type plants after watering with 200mM NaCl. The transgenic and wild-type plants were transferred to soil grown for 4 weeks under normal growth conditions and then watered with 200mM NaCl for 20 d. (B) Survival rates of AtPP2-B11 transgenic and wild-type plants. Survival rates were determined after 3 d of rewatering. Fifty plants were tested in each experiment. (C) MDA contents of AtPP2-B11 transgenic and wild-type plants. Four-week-old AtPP2-B11 transgenic and wild-type plants were treated with 200mM NaCl for 6h, and water replaced the NaCl as a control. (D, E). Chlorophyll a (D) and b (E) levels of AtPP2-B11 transgenic and wild-type plants before and after watering with 200mM NaCl for 10 d at the adult stage. Data showed the mean±SD of at least three replicates. (This figure is available in colour at JXB online.)
Mentions: To investigate further whether AtPP2-B11 functions at the adult stage, adult plants of the OE10, OE11, and R-5 lines and wild-type plants were tested for their response to high salinity. The seeds of these four genotypes were plated on ½ MS for 7 d and then transferred to soil growth for 4 weeks. Healthy and uniform transgenic and wild-type plants were selected for further treatment with or without 200mM NaCl for 20 d. Under normal growth conditions, the transgenic lines and wild-type plants showed no significant morphological or developmental abnormalities. However, when subjected to salt stress, the OE10 and OE11 lines had much greener and truer leaves, whereas a higher percentage of pale seedlings was observed in R-5 plants (Fig. 4A). Three days after rewatering, the survival ratios of the OE10 and OE11 lines were 51 and 60%, while the survival ratio of R-5 and wild-type plants were only 23 and 35%, respectively (Fig. 4B). The content of malondialdehyde (MDA) was also measured in AtPP2-B11 transgenic plants and wild-type plants, which could represent the oxidative level of membrane lipid. Although the accumulation of MDA in all four genotypes was significantly elevated under salt stress, the OE10 and OE11 lines had lower MDA levels than the wild-type and R-5 plants (Fig. 4C). In addition, the levels of chlorophyll of transgenic Arabidopsis and wild-type plants were similar under normal growth conditions. However, under salt growth conditions, the OE10 and OE11 lines had higher levels of chlorophyll a and chlorophyll b than the wild-type and R-5 plants (Fig. 4D, E). These results indicated that AtPP2-B11 acts as a positive regulator in response to salt at both the seedling and adult stages.

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.