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SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Related in: MedlinePlus

Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress in the seedling stage. (A) Primary root length of 2-week-old plants on ½ MS medium containing different NaCl concentrations. Data show the mean±SD of at least three replicates. (B) Photograph of the AtPP2-B11 transgenic and wild-type plants taken 7 d after being transferred to ½ MS medium containing 200mM NaCl. (C) Percentages of green, yellow, and pale seedlings of different genotypes exposed to 200mM NaCl. (This figure is available in colour at JXB online.)
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Figure 3: Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress in the seedling stage. (A) Primary root length of 2-week-old plants on ½ MS medium containing different NaCl concentrations. Data show the mean±SD of at least three replicates. (B) Photograph of the AtPP2-B11 transgenic and wild-type plants taken 7 d after being transferred to ½ MS medium containing 200mM NaCl. (C) Percentages of green, yellow, and pale seedlings of different genotypes exposed to 200mM NaCl. (This figure is available in colour at JXB online.)

Mentions: In order to explore the effect of salt stress on the growth of AtPP2-B11 transgenic lines at the seedling stage, root growth inhibition was measured on vertical growth plates with different concentrations of NaCl. After treatment for 2 weeks with NaCl, similar phenotypes were observed where the root length of AtPP2-B11 overexpressing plants was significantly longer than that of wild-type plants and the R-5 line, while no differences were observed when plants were grown on ½ MS agar medium (Fig. 3A). To confirm the increased tolerance of AtPP2-B11 to salt, the post-germination growth of transgenic plants was also investigated. The transgenic lines OE10, OE11, and R-5 and wild-type plants were allowed to grow on ½ MS medium for 7 d and then transferred to ½ MS medium containing 200mM NaCl for a further 7 d of growth. The percentages of green seedlings were 74 and 78% for OE10 and OE11, while wild-type and R-5 lines were only 51 and 37%, respectively (Figs. 3B, C). These results indicated that the AtPP2-B11 overexpressing lines were more tolerant to salt stress than wild-type plants both at the germination and post-germination stages, whereas the RNAi line was more sensitive to salt stress.


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress in the seedling stage. (A) Primary root length of 2-week-old plants on ½ MS medium containing different NaCl concentrations. Data show the mean±SD of at least three replicates. (B) Photograph of the AtPP2-B11 transgenic and wild-type plants taken 7 d after being transferred to ½ MS medium containing 200mM NaCl. (C) Percentages of green, yellow, and pale seedlings of different genotypes exposed to 200mM NaCl. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
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getmorefigures.php?uid=PMC4507775&req=5

Figure 3: Responses of AtPP2-B11 transgenic and wild-type (WT) plants to salt stress in the seedling stage. (A) Primary root length of 2-week-old plants on ½ MS medium containing different NaCl concentrations. Data show the mean±SD of at least three replicates. (B) Photograph of the AtPP2-B11 transgenic and wild-type plants taken 7 d after being transferred to ½ MS medium containing 200mM NaCl. (C) Percentages of green, yellow, and pale seedlings of different genotypes exposed to 200mM NaCl. (This figure is available in colour at JXB online.)
Mentions: In order to explore the effect of salt stress on the growth of AtPP2-B11 transgenic lines at the seedling stage, root growth inhibition was measured on vertical growth plates with different concentrations of NaCl. After treatment for 2 weeks with NaCl, similar phenotypes were observed where the root length of AtPP2-B11 overexpressing plants was significantly longer than that of wild-type plants and the R-5 line, while no differences were observed when plants were grown on ½ MS agar medium (Fig. 3A). To confirm the increased tolerance of AtPP2-B11 to salt, the post-germination growth of transgenic plants was also investigated. The transgenic lines OE10, OE11, and R-5 and wild-type plants were allowed to grow on ½ MS medium for 7 d and then transferred to ½ MS medium containing 200mM NaCl for a further 7 d of growth. The percentages of green seedlings were 74 and 78% for OE10 and OE11, while wild-type and R-5 lines were only 51 and 37%, respectively (Figs. 3B, C). These results indicated that the AtPP2-B11 overexpressing lines were more tolerant to salt stress than wild-type plants both at the germination and post-germination stages, whereas the RNAi line was more sensitive to salt stress.

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Related in: MedlinePlus