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SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Phenotypes of AtPP2-B11 transgenic (OE10, OE11, and R-5) and wild-type (WT) plants at the germination stage under salt stress conditions. (A) Salt response of AtPP2-B11 transgenic and wild-type plants at the germination stage. (B) Seed germination rates of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 150mM NaCl. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing different NaCl concentrations 2 d after germination. Germination rates in terms of an obvious emergence of the radicle through the seed coat were determined after the end of stratification. Data in (B) and (C) show the mean±SD of at least three replicates. For each replicate, 100 seeds per genotype were counted. (This figure is available in colour at JXB online.)
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Figure 2: Phenotypes of AtPP2-B11 transgenic (OE10, OE11, and R-5) and wild-type (WT) plants at the germination stage under salt stress conditions. (A) Salt response of AtPP2-B11 transgenic and wild-type plants at the germination stage. (B) Seed germination rates of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 150mM NaCl. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing different NaCl concentrations 2 d after germination. Germination rates in terms of an obvious emergence of the radicle through the seed coat were determined after the end of stratification. Data in (B) and (C) show the mean±SD of at least three replicates. For each replicate, 100 seeds per genotype were counted. (This figure is available in colour at JXB online.)

Mentions: To test the biological function of AtPP2-B11 in Arabidopsis, we regenerated both AtPP2-B11 overexpression transgenic lines and RNAi lines for further study. Homozygous transformant seedlings were screened, and AtPP2-B11 mRNA and proteins levels of these transgenic Arabidopsis lines were confirmed by real-time RT-PCR and Western blotting. Two overexpressing (OE) lines (OE10 and OE11) and one RNAi line (R-5) were selected for further experiments (Supplementary Fig. S1, available at JXB online). Sterilized seeds of OE10, OE11, R-5, and wild-type plants were plated on ½ MS growth medium or ½ MS medium containing 150 or 200mM NaCl, respectively. The results showed that OE10 and OE11 exhibited more salt stress tolerance than wild-type and R-5 plants at the seedling stage (Fig. 2A). The seed germination rates of transgenic Arabidopsis (OE10, OE11, and R-5) exhibited no significant differences from wild-type plants when grown under normal conditions (Fig. 2A). However, under salt stress conditions with 150mM NaCl, the two OE lines (OE10 and OE11) showed ~30% higher seed germination than wild-type plants 2 d after germination, while the R-5 line exhibited ~10% lower germination rate than wild-type plants (Fig. 2B). When the NaCl concentration was increased to 200mM, the germination rates of these four genotype seeds decreased drastically. However, the seed germination percentages of AtPP2-B11 overexpressing lines were much higher than those of wild type and the R-5 line (Fig. 2C). Although there was a delay in germination of R-5 and wild-type compared with AtPP2-B11 overexpressing lines under salt stress conditions, the germination rates overall were not affected in OE10, OE11, R-5 and wild-type plants.


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Phenotypes of AtPP2-B11 transgenic (OE10, OE11, and R-5) and wild-type (WT) plants at the germination stage under salt stress conditions. (A) Salt response of AtPP2-B11 transgenic and wild-type plants at the germination stage. (B) Seed germination rates of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 150mM NaCl. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing different NaCl concentrations 2 d after germination. Germination rates in terms of an obvious emergence of the radicle through the seed coat were determined after the end of stratification. Data in (B) and (C) show the mean±SD of at least three replicates. For each replicate, 100 seeds per genotype were counted. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 2: Phenotypes of AtPP2-B11 transgenic (OE10, OE11, and R-5) and wild-type (WT) plants at the germination stage under salt stress conditions. (A) Salt response of AtPP2-B11 transgenic and wild-type plants at the germination stage. (B) Seed germination rates of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing 150mM NaCl. (C) Seed germination percentages of AtPP2-B11 transgenic and wild-type plants grown on ½ MS medium containing different NaCl concentrations 2 d after germination. Germination rates in terms of an obvious emergence of the radicle through the seed coat were determined after the end of stratification. Data in (B) and (C) show the mean±SD of at least three replicates. For each replicate, 100 seeds per genotype were counted. (This figure is available in colour at JXB online.)
Mentions: To test the biological function of AtPP2-B11 in Arabidopsis, we regenerated both AtPP2-B11 overexpression transgenic lines and RNAi lines for further study. Homozygous transformant seedlings were screened, and AtPP2-B11 mRNA and proteins levels of these transgenic Arabidopsis lines were confirmed by real-time RT-PCR and Western blotting. Two overexpressing (OE) lines (OE10 and OE11) and one RNAi line (R-5) were selected for further experiments (Supplementary Fig. S1, available at JXB online). Sterilized seeds of OE10, OE11, R-5, and wild-type plants were plated on ½ MS growth medium or ½ MS medium containing 150 or 200mM NaCl, respectively. The results showed that OE10 and OE11 exhibited more salt stress tolerance than wild-type and R-5 plants at the seedling stage (Fig. 2A). The seed germination rates of transgenic Arabidopsis (OE10, OE11, and R-5) exhibited no significant differences from wild-type plants when grown under normal conditions (Fig. 2A). However, under salt stress conditions with 150mM NaCl, the two OE lines (OE10 and OE11) showed ~30% higher seed germination than wild-type plants 2 d after germination, while the R-5 line exhibited ~10% lower germination rate than wild-type plants (Fig. 2B). When the NaCl concentration was increased to 200mM, the germination rates of these four genotype seeds decreased drastically. However, the seed germination percentages of AtPP2-B11 overexpressing lines were much higher than those of wild type and the R-5 line (Fig. 2C). Although there was a delay in germination of R-5 and wild-type compared with AtPP2-B11 overexpressing lines under salt stress conditions, the germination rates overall were not affected in OE10, OE11, R-5 and wild-type plants.

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.