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SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.


Expression of AtPP2-B11 is induced by salt stress. (A). Induction investigation of AtPP2-B11 by salt stress at the transcriptional level. Total RNA from 2-week-old wild-type plants was obtained and analysed by real-time RT-PCR. The mean values of real-time RT-PCR from three biological replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (B). Induction investigation of AtPP2-B11 by salt stress at the protein level. Total proteins from 2-week-old wild-type plants were obtained and analysed by Western blotting. Experiments were repeated three times with similar results. UBQ10 was used as a loading control.
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Figure 1: Expression of AtPP2-B11 is induced by salt stress. (A). Induction investigation of AtPP2-B11 by salt stress at the transcriptional level. Total RNA from 2-week-old wild-type plants was obtained and analysed by real-time RT-PCR. The mean values of real-time RT-PCR from three biological replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (B). Induction investigation of AtPP2-B11 by salt stress at the protein level. Total proteins from 2-week-old wild-type plants were obtained and analysed by Western blotting. Experiments were repeated three times with similar results. UBQ10 was used as a loading control.

Mentions: To investigate the expression pattern of AtPP2-B11 under salt stress conditions, the transcript levels of AtPP2-B11 were detected by real-time RT-PCR. As shown in Fig. 1A, the expression of AtPP2-B11 in Arabidopsis seedlings was induced by treatment with 200mM NaCl. Consistent with this finding, a drastic increase in AtPP2-B11 protein was also observed with increased duration of salt treatment (Fig. 1B). The salt-induced expression of AtPP2-B11 at both the transcriptional and translational levels led us to hypothesize that AtPP2-B11 might be involved in the response to salt stress in plants.


SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.

Jia F, Wang C, Huang J, Yang G, Wu C, Zheng C - J. Exp. Bot. (2015)

Expression of AtPP2-B11 is induced by salt stress. (A). Induction investigation of AtPP2-B11 by salt stress at the transcriptional level. Total RNA from 2-week-old wild-type plants was obtained and analysed by real-time RT-PCR. The mean values of real-time RT-PCR from three biological replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (B). Induction investigation of AtPP2-B11 by salt stress at the protein level. Total proteins from 2-week-old wild-type plants were obtained and analysed by Western blotting. Experiments were repeated three times with similar results. UBQ10 was used as a loading control.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507775&req=5

Figure 1: Expression of AtPP2-B11 is induced by salt stress. (A). Induction investigation of AtPP2-B11 by salt stress at the transcriptional level. Total RNA from 2-week-old wild-type plants was obtained and analysed by real-time RT-PCR. The mean values of real-time RT-PCR from three biological replicates were normalized to the levels of an internal control, GAPDH. Error bar indicates SD (n=3). (B). Induction investigation of AtPP2-B11 by salt stress at the protein level. Total proteins from 2-week-old wild-type plants were obtained and analysed by Western blotting. Experiments were repeated three times with similar results. UBQ10 was used as a loading control.
Mentions: To investigate the expression pattern of AtPP2-B11 under salt stress conditions, the transcript levels of AtPP2-B11 were detected by real-time RT-PCR. As shown in Fig. 1A, the expression of AtPP2-B11 in Arabidopsis seedlings was induced by treatment with 200mM NaCl. Consistent with this finding, a drastic increase in AtPP2-B11 protein was also observed with increased duration of salt treatment (Fig. 1B). The salt-induced expression of AtPP2-B11 at both the transcriptional and translational levels led us to hypothesize that AtPP2-B11 might be involved in the response to salt stress in plants.

Bottom Line: Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress.Moreover, AtPP2-B11 influenced the expression of Na(+) homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na(+) accumulation.These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, PR China.

No MeSH data available.