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Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3.

Mao Z, Sun W - J. Exp. Bot. (2015)

Bottom Line: TIP3;1 and TIP3;2 promoters could be activated by ABI3 in the presence of abscisic acid (ABA) in Arabidopsis protoplasts.TIP3 proteins were detected in the protoplasts transiently expressing ABI3 and in ABI3-overexpressing seedlings when treated with ABA.Furthermore, ABI3 directly binds to the RY motif of the TIP3 promoters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Fenglin Road 300, Shanghai, 200032, People's Republic of China.

No MeSH data available.


Related in: MedlinePlus

TIP3;2 facilitates H2O2 diffusion. (A) Survival test of three different yeast strains transformed with TIP3 genes on medium containing H2O2. Yeast strains Δdur3, Δyap1, and Δskn7 were transformed with pYX212 (or derivatives of pYX212 carrying AQP cDNAs). Yeast cells were diluted to an OD600 of 0.1 with SD-Ura liquid medium, and 10 μl were spotted onto SD-Ura medium containing various concentrations of H2O2. Numbers indicate the concentration of H2O2 (mM). Photographs were taken 3 d after incubation at 30 °C. (B) TIP3;2 mediates H2O2 diffusion across the membrane in yeast. The fluorescence of CM-H2DCFDA-loaded yeast cells transformed with pYX212 or pYX212 carrying the indicated AQP cDNAs wase measured 30min after incubation with 0, 2, or 10mM H2O2. Histograms represent the average increase in fluorescence after 30min incubation. Data are means ±SD, n=3.
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Figure 7: TIP3;2 facilitates H2O2 diffusion. (A) Survival test of three different yeast strains transformed with TIP3 genes on medium containing H2O2. Yeast strains Δdur3, Δyap1, and Δskn7 were transformed with pYX212 (or derivatives of pYX212 carrying AQP cDNAs). Yeast cells were diluted to an OD600 of 0.1 with SD-Ura liquid medium, and 10 μl were spotted onto SD-Ura medium containing various concentrations of H2O2. Numbers indicate the concentration of H2O2 (mM). Photographs were taken 3 d after incubation at 30 °C. (B) TIP3;2 mediates H2O2 diffusion across the membrane in yeast. The fluorescence of CM-H2DCFDA-loaded yeast cells transformed with pYX212 or pYX212 carrying the indicated AQP cDNAs wase measured 30min after incubation with 0, 2, or 10mM H2O2. Histograms represent the average increase in fluorescence after 30min incubation. Data are means ±SD, n=3.

Mentions: To determine whether TIP3;1 and/or TIP3;2 is permeable to H2O2, yeast cells transformed with TIP3 cDNAs were grown on synthetic medium containing different concentrations of H2O2. TIP1;1 and PIP2;5, which can facilitate H2O2 diffusion across the membrane, were used as positive controls. Three yeast strains differing in H2O2 sensitivity, namely Δdur3, Δyap1, and Δskn7, were used. The result of the growth test showed that the expression of TIP3;2 significantly reduced cell growth and survival on medium containing H2O2 (Fig. 7A). When TIP3;1 was expressed in yeast, the growth of yeast cells was not significantly changed in the presence of H2O2 compared with the negative controls.


Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3.

Mao Z, Sun W - J. Exp. Bot. (2015)

TIP3;2 facilitates H2O2 diffusion. (A) Survival test of three different yeast strains transformed with TIP3 genes on medium containing H2O2. Yeast strains Δdur3, Δyap1, and Δskn7 were transformed with pYX212 (or derivatives of pYX212 carrying AQP cDNAs). Yeast cells were diluted to an OD600 of 0.1 with SD-Ura liquid medium, and 10 μl were spotted onto SD-Ura medium containing various concentrations of H2O2. Numbers indicate the concentration of H2O2 (mM). Photographs were taken 3 d after incubation at 30 °C. (B) TIP3;2 mediates H2O2 diffusion across the membrane in yeast. The fluorescence of CM-H2DCFDA-loaded yeast cells transformed with pYX212 or pYX212 carrying the indicated AQP cDNAs wase measured 30min after incubation with 0, 2, or 10mM H2O2. Histograms represent the average increase in fluorescence after 30min incubation. Data are means ±SD, n=3.
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Related In: Results  -  Collection

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Figure 7: TIP3;2 facilitates H2O2 diffusion. (A) Survival test of three different yeast strains transformed with TIP3 genes on medium containing H2O2. Yeast strains Δdur3, Δyap1, and Δskn7 were transformed with pYX212 (or derivatives of pYX212 carrying AQP cDNAs). Yeast cells were diluted to an OD600 of 0.1 with SD-Ura liquid medium, and 10 μl were spotted onto SD-Ura medium containing various concentrations of H2O2. Numbers indicate the concentration of H2O2 (mM). Photographs were taken 3 d after incubation at 30 °C. (B) TIP3;2 mediates H2O2 diffusion across the membrane in yeast. The fluorescence of CM-H2DCFDA-loaded yeast cells transformed with pYX212 or pYX212 carrying the indicated AQP cDNAs wase measured 30min after incubation with 0, 2, or 10mM H2O2. Histograms represent the average increase in fluorescence after 30min incubation. Data are means ±SD, n=3.
Mentions: To determine whether TIP3;1 and/or TIP3;2 is permeable to H2O2, yeast cells transformed with TIP3 cDNAs were grown on synthetic medium containing different concentrations of H2O2. TIP1;1 and PIP2;5, which can facilitate H2O2 diffusion across the membrane, were used as positive controls. Three yeast strains differing in H2O2 sensitivity, namely Δdur3, Δyap1, and Δskn7, were used. The result of the growth test showed that the expression of TIP3;2 significantly reduced cell growth and survival on medium containing H2O2 (Fig. 7A). When TIP3;1 was expressed in yeast, the growth of yeast cells was not significantly changed in the presence of H2O2 compared with the negative controls.

Bottom Line: TIP3;1 and TIP3;2 promoters could be activated by ABI3 in the presence of abscisic acid (ABA) in Arabidopsis protoplasts.TIP3 proteins were detected in the protoplasts transiently expressing ABI3 and in ABI3-overexpressing seedlings when treated with ABA.Furthermore, ABI3 directly binds to the RY motif of the TIP3 promoters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Fenglin Road 300, Shanghai, 200032, People's Republic of China.

No MeSH data available.


Related in: MedlinePlus