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Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3.

Mao Z, Sun W - J. Exp. Bot. (2015)

Bottom Line: TIP3;1 and TIP3;2 promoters could be activated by ABI3 in the presence of abscisic acid (ABA) in Arabidopsis protoplasts.TIP3 proteins were detected in the protoplasts transiently expressing ABI3 and in ABI3-overexpressing seedlings when treated with ABA.Furthermore, ABI3 directly binds to the RY motif of the TIP3 promoters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Fenglin Road 300, Shanghai, 200032, People's Republic of China.

No MeSH data available.


Related in: MedlinePlus

TIP3s are involved in maintaining seed viability during the CDT. (A) Seed viability after a 0–7 d CDT. Seed viability was analysed by tetrazolium staining. (B) Staining of H2O2 in the embryos of Col and tip3;1/tip3;2 seeds submitted to a CDT for 0–7 d. Seeds used for the CDT were harvested at the same time and stored for 2 weeks prior to the assay.
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Figure 4: TIP3s are involved in maintaining seed viability during the CDT. (A) Seed viability after a 0–7 d CDT. Seed viability was analysed by tetrazolium staining. (B) Staining of H2O2 in the embryos of Col and tip3;1/tip3;2 seeds submitted to a CDT for 0–7 d. Seeds used for the CDT were harvested at the same time and stored for 2 weeks prior to the assay.

Mentions: Seed longevity was further estimated using the CDT. The CDT accelerates seed ageing by increasing the temperature of seed storage and the seed moisture content. The germination percentage of all untreated seeds was ~100% at 7 d after germination. When treated at 40 °C with 80% RH for 4 d, WT, tip3;1, and tip3;2 seeds had a germination percentage of ~90%, whereas that of tip3;1/tip3;2 double mutant seeds was only 30% (Fig. 3C, E). Furthermore, the germination percentages of the seeds of three TIP3;1-RNAi/tip3;2 transgenic lines were further reduced to 1–10% (Fig. 3C, E). The seed germination percentage was <50% for tip3;1/tip3;2 and TIP3;1-RNAi/tip3;2 seeds after 3 d of the CDT, whereas for the tip3;1, tip3;2, and WT seeds, the germination percentage was <50% after 5 d of the CDT (Fig. 3C). Unlike the severe alleles of the ABI3 mutant, abi3-1 and abi3-7 seeds are desiccation tolerant but with decreased longevity (Ooms et al., 1993; Bies-Etheve et al., 1999; Tesnier et al., 2002). abi3-1 mutant seeds were also subjected to the CDT. The seed germination percentage decreased to 10% at 2 d after the CDT and to <1% after 3 d (Fig. 3D), which was even lower than that of tip3 knockdown mutant seeds. At 4 d after the CDT, abi3-1 seeds cannot germinate (Fig. 3F). A tetrazolium assay confirmed that the seeds of the tip3;1/tip3;2 double mutant and three TIP3;1-RNAi/tip3;2 transgenic lines began to lose viability 3 d after the CDT, which was earlier than observed in the WT, tip3;1, and tip3;2 mutants (Fig. 4A). Again, the abi3-1 mutant was the most sensitive to the CDT, as its seeds started to lose viability after 2 d. Taken together, these results show that TIP3s are required for seed longevity.


Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3.

Mao Z, Sun W - J. Exp. Bot. (2015)

TIP3s are involved in maintaining seed viability during the CDT. (A) Seed viability after a 0–7 d CDT. Seed viability was analysed by tetrazolium staining. (B) Staining of H2O2 in the embryos of Col and tip3;1/tip3;2 seeds submitted to a CDT for 0–7 d. Seeds used for the CDT were harvested at the same time and stored for 2 weeks prior to the assay.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 4: TIP3s are involved in maintaining seed viability during the CDT. (A) Seed viability after a 0–7 d CDT. Seed viability was analysed by tetrazolium staining. (B) Staining of H2O2 in the embryos of Col and tip3;1/tip3;2 seeds submitted to a CDT for 0–7 d. Seeds used for the CDT were harvested at the same time and stored for 2 weeks prior to the assay.
Mentions: Seed longevity was further estimated using the CDT. The CDT accelerates seed ageing by increasing the temperature of seed storage and the seed moisture content. The germination percentage of all untreated seeds was ~100% at 7 d after germination. When treated at 40 °C with 80% RH for 4 d, WT, tip3;1, and tip3;2 seeds had a germination percentage of ~90%, whereas that of tip3;1/tip3;2 double mutant seeds was only 30% (Fig. 3C, E). Furthermore, the germination percentages of the seeds of three TIP3;1-RNAi/tip3;2 transgenic lines were further reduced to 1–10% (Fig. 3C, E). The seed germination percentage was <50% for tip3;1/tip3;2 and TIP3;1-RNAi/tip3;2 seeds after 3 d of the CDT, whereas for the tip3;1, tip3;2, and WT seeds, the germination percentage was <50% after 5 d of the CDT (Fig. 3C). Unlike the severe alleles of the ABI3 mutant, abi3-1 and abi3-7 seeds are desiccation tolerant but with decreased longevity (Ooms et al., 1993; Bies-Etheve et al., 1999; Tesnier et al., 2002). abi3-1 mutant seeds were also subjected to the CDT. The seed germination percentage decreased to 10% at 2 d after the CDT and to <1% after 3 d (Fig. 3D), which was even lower than that of tip3 knockdown mutant seeds. At 4 d after the CDT, abi3-1 seeds cannot germinate (Fig. 3F). A tetrazolium assay confirmed that the seeds of the tip3;1/tip3;2 double mutant and three TIP3;1-RNAi/tip3;2 transgenic lines began to lose viability 3 d after the CDT, which was earlier than observed in the WT, tip3;1, and tip3;2 mutants (Fig. 4A). Again, the abi3-1 mutant was the most sensitive to the CDT, as its seeds started to lose viability after 2 d. Taken together, these results show that TIP3s are required for seed longevity.

Bottom Line: TIP3;1 and TIP3;2 promoters could be activated by ABI3 in the presence of abscisic acid (ABA) in Arabidopsis protoplasts.TIP3 proteins were detected in the protoplasts transiently expressing ABI3 and in ABI3-overexpressing seedlings when treated with ABA.Furthermore, ABI3 directly binds to the RY motif of the TIP3 promoters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Fenglin Road 300, Shanghai, 200032, People's Republic of China.

No MeSH data available.


Related in: MedlinePlus