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Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3.

Mao Z, Sun W - J. Exp. Bot. (2015)

Bottom Line: TIP3;1 and TIP3;2 promoters could be activated by ABI3 in the presence of abscisic acid (ABA) in Arabidopsis protoplasts.TIP3 proteins were detected in the protoplasts transiently expressing ABI3 and in ABI3-overexpressing seedlings when treated with ABA.Furthermore, ABI3 directly binds to the RY motif of the TIP3 promoters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Fenglin Road 300, Shanghai, 200032, People's Republic of China.

No MeSH data available.


Related in: MedlinePlus

Natural and artificial seed ageing assays showing that TIP3 genes are involved in maintaining seed longevity. (A) Germination and growth of seeds from Col, tip3;2, and three lines of TIP3;1-RNAi/tip3;2 stored for 2 weeks or 18 months. The photographs were taken 6 d after seed imbibition. (B) Germination percentages of seeds 6 d after imbibition. (C) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (D) Germination percentages of Ler and abi3-1 seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (E) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. (F) Germination percentages of Ler and abi3-1 seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. Values are the means ±SD of four technical replicates with 100 seeds per replicate. In (C–E) seeds used for germination tests were harvested at the same time and stored for 2 weeks prior to the experiment.
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Figure 3: Natural and artificial seed ageing assays showing that TIP3 genes are involved in maintaining seed longevity. (A) Germination and growth of seeds from Col, tip3;2, and three lines of TIP3;1-RNAi/tip3;2 stored for 2 weeks or 18 months. The photographs were taken 6 d after seed imbibition. (B) Germination percentages of seeds 6 d after imbibition. (C) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (D) Germination percentages of Ler and abi3-1 seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (E) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. (F) Germination percentages of Ler and abi3-1 seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. Values are the means ±SD of four technical replicates with 100 seeds per replicate. In (C–E) seeds used for germination tests were harvested at the same time and stored for 2 weeks prior to the experiment.

Mentions: The seed germination percentage of TIP3;1-RNAi/tip3;2 plants was tested. No significant difference was observed between TIP3;1-RNAi/tip3;2 and WT seeds which were stored for 2 weeks after harvesting (Fig. 3A). Comparing the germination percentage for 18-month-old seeds, WT and tip3;2 seeds remained at 98% and 95%, respectively. In contrast, the germination percentage of TIP3;1-RNAi/tip3;2 seeds decreased to <40% (Fig. 3A, B).


Arabidopsis seed-specific vacuolar aquaporins are involved in maintaining seed longevity under the control of ABSCISIC ACID INSENSITIVE 3.

Mao Z, Sun W - J. Exp. Bot. (2015)

Natural and artificial seed ageing assays showing that TIP3 genes are involved in maintaining seed longevity. (A) Germination and growth of seeds from Col, tip3;2, and three lines of TIP3;1-RNAi/tip3;2 stored for 2 weeks or 18 months. The photographs were taken 6 d after seed imbibition. (B) Germination percentages of seeds 6 d after imbibition. (C) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (D) Germination percentages of Ler and abi3-1 seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (E) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. (F) Germination percentages of Ler and abi3-1 seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. Values are the means ±SD of four technical replicates with 100 seeds per replicate. In (C–E) seeds used for germination tests were harvested at the same time and stored for 2 weeks prior to the experiment.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507774&req=5

Figure 3: Natural and artificial seed ageing assays showing that TIP3 genes are involved in maintaining seed longevity. (A) Germination and growth of seeds from Col, tip3;2, and three lines of TIP3;1-RNAi/tip3;2 stored for 2 weeks or 18 months. The photographs were taken 6 d after seed imbibition. (B) Germination percentages of seeds 6 d after imbibition. (C) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (D) Germination percentages of Ler and abi3-1 seeds submitted to a CDT for 1–7 d. The germination percentages were counted 7 d after imbibition. (E) Germination percentages of tip3 mutants and TIP3;1-RNAi/tip3;2 transgenic seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. (F) Germination percentages of Ler and abi3-1 seeds after a 4 d CDT. Germination percentages were counted at different time points after imbibition. Values are the means ±SD of four technical replicates with 100 seeds per replicate. In (C–E) seeds used for germination tests were harvested at the same time and stored for 2 weeks prior to the experiment.
Mentions: The seed germination percentage of TIP3;1-RNAi/tip3;2 plants was tested. No significant difference was observed between TIP3;1-RNAi/tip3;2 and WT seeds which were stored for 2 weeks after harvesting (Fig. 3A). Comparing the germination percentage for 18-month-old seeds, WT and tip3;2 seeds remained at 98% and 95%, respectively. In contrast, the germination percentage of TIP3;1-RNAi/tip3;2 seeds decreased to <40% (Fig. 3A, B).

Bottom Line: TIP3;1 and TIP3;2 promoters could be activated by ABI3 in the presence of abscisic acid (ABA) in Arabidopsis protoplasts.TIP3 proteins were detected in the protoplasts transiently expressing ABI3 and in ABI3-overexpressing seedlings when treated with ABA.Furthermore, ABI3 directly binds to the RY motif of the TIP3 promoters.

View Article: PubMed Central - PubMed

Affiliation: Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Fenglin Road 300, Shanghai, 200032, People's Republic of China.

No MeSH data available.


Related in: MedlinePlus