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Regulation of flavonol content and composition in (Syrah×Pinot Noir) mature grapes: integration of transcriptional profiling and metabolic quantitative trait locus analyses.

Malacarne G, Costantini L, Coller E, Battilana J, Velasco R, Vrhovsek U, Grando MS, Moser C - J. Exp. Bot. (2015)

Bottom Line: Moreover, seven regions specifically associated with the fine control of flavonol biosynthesis are identified.Gene expression profiling of two groups of individuals significantly divergent for their skin flavonol content identified a large set of differentially modulated transcripts.Among these, the transcripts coding for MYB and bZIP transcription factors, methyltranferases, and glucosyltranferases specific for flavonols, proteins, and factors belonging to the UV-B signalling pathway and co-localizing with the QTL regions are proposed as candidate genes for the fine regulation of flavonol content and composition in mature grapes.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Biology of Fruit Crops Department, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy giulia.malacarne@fmach.it.

No MeSH data available.


Variation of the flavonol content in the parental lines (A) and in the ‘Syrah’x‘Pinot Noir’ progeny (B) in berry skins at mature stage. (A) Profile of each flavonol in each year in the two parents of the cross. (B) Distribution of the progeny in classes at increasing content of each flavonol (mg kg–1 berry). In the right upper part of each plot, the Spearman rank-order correlation between years (r) (mean value and range of variation in parentheses) is shown for the whole progeny. Correlations are significant at the 0.01 level. For the two parents and for high- and low-flavonol producers, averaged values across years and across genotypes (in the second case) are reported. Abbreviations: Sy, Syrah; PN, Pinot Noir; LFPs, low-flavonol producers; HFPs, high-flavonol producers; WS, white-skinned individuals; RS, red-skinned individuals.
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Figure 1: Variation of the flavonol content in the parental lines (A) and in the ‘Syrah’x‘Pinot Noir’ progeny (B) in berry skins at mature stage. (A) Profile of each flavonol in each year in the two parents of the cross. (B) Distribution of the progeny in classes at increasing content of each flavonol (mg kg–1 berry). In the right upper part of each plot, the Spearman rank-order correlation between years (r) (mean value and range of variation in parentheses) is shown for the whole progeny. Correlations are significant at the 0.01 level. For the two parents and for high- and low-flavonol producers, averaged values across years and across genotypes (in the second case) are reported. Abbreviations: Sy, Syrah; PN, Pinot Noir; LFPs, low-flavonol producers; HFPs, high-flavonol producers; WS, white-skinned individuals; RS, red-skinned individuals.

Mentions: The total flavonol content in the four seasons was on average three times higher in ‘Syrah’ than in ‘Pinot Noir’, ranging from 34mg kg–1 to 57mg kg–1 for ‘Syrah’ and from 5mg kg–1 to 23mg kg–1 for ‘Pinot Noir’. In particular, Que and Myr were the most abundant flavonols in all the seasons, with Que being on average three and four times higher than Myr in ‘Pinot Noir’ and ‘Syrah’, respectively (Fig. 1A). The profiles among years were relatively stable in both cultivars, with few exceptions: in 2009, Kaemp, Isor, and Lar were completely undetectable in ‘Pinot Noir’, possibly compensated by an increase in Myr, with the latter being very low in ‘Syrah’ in 2011 (Fig. 1A). Moreover, looking at the ratios between tri-hydroxylated and di-hydroxylated flavonols (triOH/diOH), between 3′-methylated and 3′-hydroxylated flavonols (3′Meth/3′OH), and between 3′,5′-methylated and 3′,5′-hydroxylated flavonols (5′Meth/5′OH), it appears that they were on average higher in ‘Syrah’ than in ‘Pinot Noir’.


Regulation of flavonol content and composition in (Syrah×Pinot Noir) mature grapes: integration of transcriptional profiling and metabolic quantitative trait locus analyses.

Malacarne G, Costantini L, Coller E, Battilana J, Velasco R, Vrhovsek U, Grando MS, Moser C - J. Exp. Bot. (2015)

Variation of the flavonol content in the parental lines (A) and in the ‘Syrah’x‘Pinot Noir’ progeny (B) in berry skins at mature stage. (A) Profile of each flavonol in each year in the two parents of the cross. (B) Distribution of the progeny in classes at increasing content of each flavonol (mg kg–1 berry). In the right upper part of each plot, the Spearman rank-order correlation between years (r) (mean value and range of variation in parentheses) is shown for the whole progeny. Correlations are significant at the 0.01 level. For the two parents and for high- and low-flavonol producers, averaged values across years and across genotypes (in the second case) are reported. Abbreviations: Sy, Syrah; PN, Pinot Noir; LFPs, low-flavonol producers; HFPs, high-flavonol producers; WS, white-skinned individuals; RS, red-skinned individuals.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507773&req=5

Figure 1: Variation of the flavonol content in the parental lines (A) and in the ‘Syrah’x‘Pinot Noir’ progeny (B) in berry skins at mature stage. (A) Profile of each flavonol in each year in the two parents of the cross. (B) Distribution of the progeny in classes at increasing content of each flavonol (mg kg–1 berry). In the right upper part of each plot, the Spearman rank-order correlation between years (r) (mean value and range of variation in parentheses) is shown for the whole progeny. Correlations are significant at the 0.01 level. For the two parents and for high- and low-flavonol producers, averaged values across years and across genotypes (in the second case) are reported. Abbreviations: Sy, Syrah; PN, Pinot Noir; LFPs, low-flavonol producers; HFPs, high-flavonol producers; WS, white-skinned individuals; RS, red-skinned individuals.
Mentions: The total flavonol content in the four seasons was on average three times higher in ‘Syrah’ than in ‘Pinot Noir’, ranging from 34mg kg–1 to 57mg kg–1 for ‘Syrah’ and from 5mg kg–1 to 23mg kg–1 for ‘Pinot Noir’. In particular, Que and Myr were the most abundant flavonols in all the seasons, with Que being on average three and four times higher than Myr in ‘Pinot Noir’ and ‘Syrah’, respectively (Fig. 1A). The profiles among years were relatively stable in both cultivars, with few exceptions: in 2009, Kaemp, Isor, and Lar were completely undetectable in ‘Pinot Noir’, possibly compensated by an increase in Myr, with the latter being very low in ‘Syrah’ in 2011 (Fig. 1A). Moreover, looking at the ratios between tri-hydroxylated and di-hydroxylated flavonols (triOH/diOH), between 3′-methylated and 3′-hydroxylated flavonols (3′Meth/3′OH), and between 3′,5′-methylated and 3′,5′-hydroxylated flavonols (5′Meth/5′OH), it appears that they were on average higher in ‘Syrah’ than in ‘Pinot Noir’.

Bottom Line: Moreover, seven regions specifically associated with the fine control of flavonol biosynthesis are identified.Gene expression profiling of two groups of individuals significantly divergent for their skin flavonol content identified a large set of differentially modulated transcripts.Among these, the transcripts coding for MYB and bZIP transcription factors, methyltranferases, and glucosyltranferases specific for flavonols, proteins, and factors belonging to the UV-B signalling pathway and co-localizing with the QTL regions are proposed as candidate genes for the fine regulation of flavonol content and composition in mature grapes.

View Article: PubMed Central - PubMed

Affiliation: Genomics and Biology of Fruit Crops Department, Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 S. Michele all'Adige, Trento, Italy giulia.malacarne@fmach.it.

No MeSH data available.