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A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Relationship between GhNAP and ABA pathways. (A) Distribution of cis-elements in the promoter region of GhNAP. The main cis-elements are indicated as follows: filled circles, ABRE; filled inverted triangles, MYC recognition site; open triangles, MYB recognition site; open diamonds, CAAT. (B) Effects of ABA on GhNAP expression in cotton. (C) Endogenous levels of ABA in WT and GhNAPi lines. (D–G) Expression of ABA-related genes in wild-type and GhNAPi plants. (G) Interaction between GhNAP and the promoter of GhSAG113 by Y1H assay. (This figure is available in colour at JXB online.)
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Figure 8: Relationship between GhNAP and ABA pathways. (A) Distribution of cis-elements in the promoter region of GhNAP. The main cis-elements are indicated as follows: filled circles, ABRE; filled inverted triangles, MYC recognition site; open triangles, MYB recognition site; open diamonds, CAAT. (B) Effects of ABA on GhNAP expression in cotton. (C) Endogenous levels of ABA in WT and GhNAPi lines. (D–G) Expression of ABA-related genes in wild-type and GhNAPi plants. (G) Interaction between GhNAP and the promoter of GhSAG113 by Y1H assay. (This figure is available in colour at JXB online.)

Mentions: To elucidate the possible regulatory mechanism, some putative cis-elements were identified in the promoter region of GhNAP (Fig. 8A). Surprisingly, not only the common cis-element (CAAT-box), but also several specific cis-elements related to the ABA responses (ABRE, and recognition sites for MYB and MYC) existed in the promoter region of GhNAP. In addition, qRT-PCR analysis showed that the expression pattern of GhNAP was highly increased after ABA treatment (Fig. 8B). To explore further the function of GhNAP in the ABA responses, the endogenous ABA level was measured in GhNAPi and wild-type leaves. The results showed that the ABA content was significantly lower in GhNAPi than in wild-type leaves (Fig. 8C). Furthermore, the expression levels of ABA-related genes were analysed in GhNAPi and wild-type lines. Compared with the wild-type lines, GhSAG113, GhMYC2, and GhMYB2 exhibited remarkably decreased expression levels in the GhNAPi plants (Fig. 8D–F). In particular, the expression level of GhSAG113 was reduced >70% in GhNAPi plants. However, the other four genes did not show a significant difference between GhNAPi and wild-type plants (Supplementary Fig. S10 at JXB online). Then, the Y1H assay was performed to investigate the interaction between GhNAP and GhSAG113 (Fig. 8D). The promoter regions of GhSAG113 (1000bp) were isolated and introduced into the genome of the Y1H Gold strain as the bait reporter strain. Subsequently, the pGADT7-GhNAP vector was transformed into the bait reporter strain. The transformants exhibited normal growth on SD/–Leu medium, but could not grow on SD/–Leu/AbA medium.


A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Relationship between GhNAP and ABA pathways. (A) Distribution of cis-elements in the promoter region of GhNAP. The main cis-elements are indicated as follows: filled circles, ABRE; filled inverted triangles, MYC recognition site; open triangles, MYB recognition site; open diamonds, CAAT. (B) Effects of ABA on GhNAP expression in cotton. (C) Endogenous levels of ABA in WT and GhNAPi lines. (D–G) Expression of ABA-related genes in wild-type and GhNAPi plants. (G) Interaction between GhNAP and the promoter of GhSAG113 by Y1H assay. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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Figure 8: Relationship between GhNAP and ABA pathways. (A) Distribution of cis-elements in the promoter region of GhNAP. The main cis-elements are indicated as follows: filled circles, ABRE; filled inverted triangles, MYC recognition site; open triangles, MYB recognition site; open diamonds, CAAT. (B) Effects of ABA on GhNAP expression in cotton. (C) Endogenous levels of ABA in WT and GhNAPi lines. (D–G) Expression of ABA-related genes in wild-type and GhNAPi plants. (G) Interaction between GhNAP and the promoter of GhSAG113 by Y1H assay. (This figure is available in colour at JXB online.)
Mentions: To elucidate the possible regulatory mechanism, some putative cis-elements were identified in the promoter region of GhNAP (Fig. 8A). Surprisingly, not only the common cis-element (CAAT-box), but also several specific cis-elements related to the ABA responses (ABRE, and recognition sites for MYB and MYC) existed in the promoter region of GhNAP. In addition, qRT-PCR analysis showed that the expression pattern of GhNAP was highly increased after ABA treatment (Fig. 8B). To explore further the function of GhNAP in the ABA responses, the endogenous ABA level was measured in GhNAPi and wild-type leaves. The results showed that the ABA content was significantly lower in GhNAPi than in wild-type leaves (Fig. 8C). Furthermore, the expression levels of ABA-related genes were analysed in GhNAPi and wild-type lines. Compared with the wild-type lines, GhSAG113, GhMYC2, and GhMYB2 exhibited remarkably decreased expression levels in the GhNAPi plants (Fig. 8D–F). In particular, the expression level of GhSAG113 was reduced >70% in GhNAPi plants. However, the other four genes did not show a significant difference between GhNAPi and wild-type plants (Supplementary Fig. S10 at JXB online). Then, the Y1H assay was performed to investigate the interaction between GhNAP and GhSAG113 (Fig. 8D). The promoter regions of GhSAG113 (1000bp) were isolated and introduced into the genome of the Y1H Gold strain as the bait reporter strain. Subsequently, the pGADT7-GhNAP vector was transformed into the bait reporter strain. The transformants exhibited normal growth on SD/–Leu medium, but could not grow on SD/–Leu/AbA medium.

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.