Limits...
A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Physiological and molecular analysis of cotton leaf in wild-type (WT) and GhNAPi lines. (A–E) Chlorophyll content (A), SPAD value (B), net photosynthetic rate (Pn; C), intercellular CO2 concentration (Ci; D), and Fv/Fm ratio (E) of cotton leaf from 60 to 150 DAP. (F) Emission spectra image on Fv/Fm of the corresponding leaf at 120 DAP. The bar on the bottom shows the Fv/Fm value. (G, H) Relative expression of GhNAP and GhCAB at 120 DAP. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4507772&req=5

Figure 7: Physiological and molecular analysis of cotton leaf in wild-type (WT) and GhNAPi lines. (A–E) Chlorophyll content (A), SPAD value (B), net photosynthetic rate (Pn; C), intercellular CO2 concentration (Ci; D), and Fv/Fm ratio (E) of cotton leaf from 60 to 150 DAP. (F) Emission spectra image on Fv/Fm of the corresponding leaf at 120 DAP. The bar on the bottom shows the Fv/Fm value. (G, H) Relative expression of GhNAP and GhCAB at 120 DAP. (This figure is available in colour at JXB online.)

Mentions: As the striking phenotypic change, leaf yellowing was measured to investigate the leaf senescence every 15 d from 60 days after planting (DAP) to 150 DAP across the main growth period of cotton. At 120 DAP, leaves of GhNAPi had a much higher chlorophyll content and SPAD value than those of the wild type (Fig. 7A, B). The net photosynthetic rate (Pn) and the Fv/Fm ratio increased at first and gradually declined after 105 DAP, but at 120 DAP the decline of Pn and the Fv/Fm ratio was significantly slower in GhNAPi than that in the wild type (Fig. 7C, E, F). However, the intercellular CO2 concentration (Ci) showed the opposite effects (Fig. 7D). Moreover, GhNAP expression was significantly lower at 120 DAP in GhNAPi than in the wild type (Fig. 7G), but GhCAB showed the opposite tendency (Fig. 7H). The MDA content, soluble protein content, SOD activity, and POD activity showed a significant difference at 120 DAP between the wild type and GhNAPi (Supplementary Fig. S8 at JXB online). In addition, the detached leaves were induced by being kept in the dark for 3 d (Supplementayr Fig. S9A, B). Higher chlorophyll content and lower membrane ion leakage were measured in GhNAPi than in the wild type (Supplementary Fig. S9C, D). The relative expression level of GhNAP and GhCAB showed similar results (Supplementary Fig. S9E, F).


A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Physiological and molecular analysis of cotton leaf in wild-type (WT) and GhNAPi lines. (A–E) Chlorophyll content (A), SPAD value (B), net photosynthetic rate (Pn; C), intercellular CO2 concentration (Ci; D), and Fv/Fm ratio (E) of cotton leaf from 60 to 150 DAP. (F) Emission spectra image on Fv/Fm of the corresponding leaf at 120 DAP. The bar on the bottom shows the Fv/Fm value. (G, H) Relative expression of GhNAP and GhCAB at 120 DAP. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507772&req=5

Figure 7: Physiological and molecular analysis of cotton leaf in wild-type (WT) and GhNAPi lines. (A–E) Chlorophyll content (A), SPAD value (B), net photosynthetic rate (Pn; C), intercellular CO2 concentration (Ci; D), and Fv/Fm ratio (E) of cotton leaf from 60 to 150 DAP. (F) Emission spectra image on Fv/Fm of the corresponding leaf at 120 DAP. The bar on the bottom shows the Fv/Fm value. (G, H) Relative expression of GhNAP and GhCAB at 120 DAP. (This figure is available in colour at JXB online.)
Mentions: As the striking phenotypic change, leaf yellowing was measured to investigate the leaf senescence every 15 d from 60 days after planting (DAP) to 150 DAP across the main growth period of cotton. At 120 DAP, leaves of GhNAPi had a much higher chlorophyll content and SPAD value than those of the wild type (Fig. 7A, B). The net photosynthetic rate (Pn) and the Fv/Fm ratio increased at first and gradually declined after 105 DAP, but at 120 DAP the decline of Pn and the Fv/Fm ratio was significantly slower in GhNAPi than that in the wild type (Fig. 7C, E, F). However, the intercellular CO2 concentration (Ci) showed the opposite effects (Fig. 7D). Moreover, GhNAP expression was significantly lower at 120 DAP in GhNAPi than in the wild type (Fig. 7G), but GhCAB showed the opposite tendency (Fig. 7H). The MDA content, soluble protein content, SOD activity, and POD activity showed a significant difference at 120 DAP between the wild type and GhNAPi (Supplementary Fig. S8 at JXB online). In addition, the detached leaves were induced by being kept in the dark for 3 d (Supplementayr Fig. S9A, B). Higher chlorophyll content and lower membrane ion leakage were measured in GhNAPi than in the wild type (Supplementary Fig. S9C, D). The relative expression level of GhNAP and GhCAB showed similar results (Supplementary Fig. S9E, F).

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.