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A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus

Natural senescence process of Arabidopsis leaves in Col-0, atnap, and GhNAPi lines. (A, B) Phenotypes of Col-0, atnap, and GhNAPi lines under a normal environment after 50 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the five groups. (This figure is available in colour at JXB online.)
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Figure 5: Natural senescence process of Arabidopsis leaves in Col-0, atnap, and GhNAPi lines. (A, B) Phenotypes of Col-0, atnap, and GhNAPi lines under a normal environment after 50 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the five groups. (This figure is available in colour at JXB online.)

Mentions: In addition, the GhNAP interference vector (pCI-GhNAPi) was transformed into Col-0 (Supplementary Fig. S3 at JXB online). After 35 d growth, leaf senescence occurred first in Col-0. Subsequently, GhNAPi leaves started yellowing after 40 d. After 50 d, leaf senescence was seen in all lines, and detached leaves were divided into five groups (G1–G5) (Fig. 5A, B). The chlorophyll loss and membrane ion leakage in the G1 and G2 leaves of GhNAPi were significantly lower than in the counterpart leaves of Col-0, but higher than those of atnap (Fig. 5C, D). A similar ageing process was also shown by the relative expression level of AtNAP, AtSAG12, and AtCAB (Fig. 5E–G). Then the fifth rosette leaves of three lines were incubated in the dark for 5 d (Supplementary Fig. S6). All of these leaves exhibited the senescent phenotype, but the highest level of leaf senescence was observed in Col-0, followed by GhNAPi, and then atnap.


A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Natural senescence process of Arabidopsis leaves in Col-0, atnap, and GhNAPi lines. (A, B) Phenotypes of Col-0, atnap, and GhNAPi lines under a normal environment after 50 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the five groups. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507772&req=5

Figure 5: Natural senescence process of Arabidopsis leaves in Col-0, atnap, and GhNAPi lines. (A, B) Phenotypes of Col-0, atnap, and GhNAPi lines under a normal environment after 50 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the five groups. (This figure is available in colour at JXB online.)
Mentions: In addition, the GhNAP interference vector (pCI-GhNAPi) was transformed into Col-0 (Supplementary Fig. S3 at JXB online). After 35 d growth, leaf senescence occurred first in Col-0. Subsequently, GhNAPi leaves started yellowing after 40 d. After 50 d, leaf senescence was seen in all lines, and detached leaves were divided into five groups (G1–G5) (Fig. 5A, B). The chlorophyll loss and membrane ion leakage in the G1 and G2 leaves of GhNAPi were significantly lower than in the counterpart leaves of Col-0, but higher than those of atnap (Fig. 5C, D). A similar ageing process was also shown by the relative expression level of AtNAP, AtSAG12, and AtCAB (Fig. 5E–G). Then the fifth rosette leaves of three lines were incubated in the dark for 5 d (Supplementary Fig. S6). All of these leaves exhibited the senescent phenotype, but the highest level of leaf senescence was observed in Col-0, followed by GhNAPi, and then atnap.

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus