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A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus

Natural senescence process of Arabidopsis leaves in Col-0 and GhNAP lines. (A, B) Phenotypes of Col-0 and GhNAP lines under a normal environment after 30 d growth. G1–G3, three groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the three groups. (This figure is available in colour at JXB online.)
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Figure 4: Natural senescence process of Arabidopsis leaves in Col-0 and GhNAP lines. (A, B) Phenotypes of Col-0 and GhNAP lines under a normal environment after 30 d growth. G1–G3, three groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the three groups. (This figure is available in colour at JXB online.)

Mentions: The GhNAP coding sequence, driven by the 35S promoter, was introduced into the wild-type Col-0 to overexpress GhNAP (Supplementary Fig. S3 at JXB online). After 20 d growth, the GhNAP overexpressors displayed the senescence phenotype, while the no yellowing phenotype was found in Col-0. After 30 d, the detached leaves were divided into three groups (G1–G3) (Fig. 4A, B). Compared with Col-0, the GhNAP lines showed much lower chlorophyll contents in the G1 and G2 leaves (Fig. 4C), while the GhNAP lines had much higher membrane ion leakage (Fig. 4D). The relative expression level of AtNAP, AtSAG12, and AtCAB also reflected the similar senescing process (Fig. 4E–G). Furthermore, compared with the Col-0 leaves, the GhNAP leaves showed the precocious senescence phenotype after 3 d in darkness (Supplementary Fig. S5).


A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Natural senescence process of Arabidopsis leaves in Col-0 and GhNAP lines. (A, B) Phenotypes of Col-0 and GhNAP lines under a normal environment after 30 d growth. G1–G3, three groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the three groups. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507772&req=5

Figure 4: Natural senescence process of Arabidopsis leaves in Col-0 and GhNAP lines. (A, B) Phenotypes of Col-0 and GhNAP lines under a normal environment after 30 d growth. G1–G3, three groups of detached leaves according to the senescent condition. (C–G) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtNAP (E), AtSAG12 (F), and AtCAB (G) in the detached leaves of the three groups. (This figure is available in colour at JXB online.)
Mentions: The GhNAP coding sequence, driven by the 35S promoter, was introduced into the wild-type Col-0 to overexpress GhNAP (Supplementary Fig. S3 at JXB online). After 20 d growth, the GhNAP overexpressors displayed the senescence phenotype, while the no yellowing phenotype was found in Col-0. After 30 d, the detached leaves were divided into three groups (G1–G3) (Fig. 4A, B). Compared with Col-0, the GhNAP lines showed much lower chlorophyll contents in the G1 and G2 leaves (Fig. 4C), while the GhNAP lines had much higher membrane ion leakage (Fig. 4D). The relative expression level of AtNAP, AtSAG12, and AtCAB also reflected the similar senescing process (Fig. 4E–G). Furthermore, compared with the Col-0 leaves, the GhNAP leaves showed the precocious senescence phenotype after 3 d in darkness (Supplementary Fig. S5).

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus