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A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus

Natural senescence of GhNAP-complemented lines. (A, B) Phenotypes of Col-0 and GhNAP_RE lines under a normal environment after 60 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–F) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtSAG12 (E) and AtCAB (F) in the detached leaves of the five groups. AtActin2 was used as the standard control in all of the qRT-PCR experiments in Arabidopsis. (This figure is available in colour at JXB online.)
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Figure 3: Natural senescence of GhNAP-complemented lines. (A, B) Phenotypes of Col-0 and GhNAP_RE lines under a normal environment after 60 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–F) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtSAG12 (E) and AtCAB (F) in the detached leaves of the five groups. AtActin2 was used as the standard control in all of the qRT-PCR experiments in Arabidopsis. (This figure is available in colour at JXB online.)

Mentions: Previous reports have shown that the atnap mutant can delay leaf senescence (Guo and Gan, 2006). To test whether GhNAP is a functional homologue of AtNAP, the GhNAP ORF, driven by the promoter region of AtNAP, was transformed into the atnap mutant (Supplementary Fig. S3 at JXB online). The leaves from the GhNAP-complemented lines (GhNAP _RE) senesced in a similar pattern to the leaves from Col-0, but senesced much more quickly than the leaves from atnap, both phenotypically (Fig. 3A, B) and in terms of chlorophyll content (Fig. 3C), membrane ion leakage (Fig. 3D), and relative expression of AtSAG12 (Fig. 3E) and AtCAB (Fig. 3F). After dark treatment for 5 d, detached leaves of GhNAP _RE exhibited wild-type-like yellowing (Supplementary Fig. S4).


A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Natural senescence of GhNAP-complemented lines. (A, B) Phenotypes of Col-0 and GhNAP_RE lines under a normal environment after 60 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–F) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtSAG12 (E) and AtCAB (F) in the detached leaves of the five groups. AtActin2 was used as the standard control in all of the qRT-PCR experiments in Arabidopsis. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507772&req=5

Figure 3: Natural senescence of GhNAP-complemented lines. (A, B) Phenotypes of Col-0 and GhNAP_RE lines under a normal environment after 60 d growth. G1–G5, five groups of detached leaves according to the senescent condition. (C–F) Chlorophyll content (C), membrane ion leakage (D), and relative expression of AtSAG12 (E) and AtCAB (F) in the detached leaves of the five groups. AtActin2 was used as the standard control in all of the qRT-PCR experiments in Arabidopsis. (This figure is available in colour at JXB online.)
Mentions: Previous reports have shown that the atnap mutant can delay leaf senescence (Guo and Gan, 2006). To test whether GhNAP is a functional homologue of AtNAP, the GhNAP ORF, driven by the promoter region of AtNAP, was transformed into the atnap mutant (Supplementary Fig. S3 at JXB online). The leaves from the GhNAP-complemented lines (GhNAP _RE) senesced in a similar pattern to the leaves from Col-0, but senesced much more quickly than the leaves from atnap, both phenotypically (Fig. 3A, B) and in terms of chlorophyll content (Fig. 3C), membrane ion leakage (Fig. 3D), and relative expression of AtSAG12 (Fig. 3E) and AtCAB (Fig. 3F). After dark treatment for 5 d, detached leaves of GhNAP _RE exhibited wild-type-like yellowing (Supplementary Fig. S4).

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus