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A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus

Physiological and molecular analysis of GhNAP expression during leaf senescence in cotton. (A–E) Cotton leaves at various developmental stages and GhNAP expression. YL, a young leaf half the size of a fully expanded leaf; NS, a fully expanded, non-senescent leaf; ES, an early senescent leaf, with <50% leaf area yellowing; LS, a late senescent leaf, with >50% leaf area yellowing. Chlorophyll content (B), membrane ion leakage (C), and relative expression of GhNAP (D) and GhCAB (E) at the corresponding stage of (A). Error bars indicate the standard error (n=3). Significant differences between means are represented by different letters. A similar statistical analysis was conducted in the following parts. (F–H) Different parts in the senescing cotton leaf and the relative expression level of GhNAP and GhCAB. B, base; M, middle; T, tip. EF1α was used as the standard control in all qRT-PCR experiments in cotton. (This figure is available in colour at JXB online.)
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Figure 2: Physiological and molecular analysis of GhNAP expression during leaf senescence in cotton. (A–E) Cotton leaves at various developmental stages and GhNAP expression. YL, a young leaf half the size of a fully expanded leaf; NS, a fully expanded, non-senescent leaf; ES, an early senescent leaf, with <50% leaf area yellowing; LS, a late senescent leaf, with >50% leaf area yellowing. Chlorophyll content (B), membrane ion leakage (C), and relative expression of GhNAP (D) and GhCAB (E) at the corresponding stage of (A). Error bars indicate the standard error (n=3). Significant differences between means are represented by different letters. A similar statistical analysis was conducted in the following parts. (F–H) Different parts in the senescing cotton leaf and the relative expression level of GhNAP and GhCAB. B, base; M, middle; T, tip. EF1α was used as the standard control in all qRT-PCR experiments in cotton. (This figure is available in colour at JXB online.)

Mentions: The expression of GhNAP was examined in young leaves (YL), non-senescent leaves (NS), early senescent leaves (ES), and late senescent leaves (LS) (Fig. 2A). Consistent with the leaf phenotype, the chlorophyll loss and membrane ion leakage of ES and LS were higher than those of YL and NS (Fig. 2B, C). Moreover, compared with YL and NL, the expression of GhNAP was higher in ES and LS, whereas GhCAB, a negative senescence marker gene, was expressed at a lower level in the same leaves (Fig. 2D, E). During the senescence process (Fig. 2F), more GhNAP expression was detected in the yellow tip (T) than in other parts (Fig. 2G). The present findings were further strengthened by the opposite expression level of GhCAB (Fig. 2H). Furthermore, YL were treated in a dark environment for 7 d. The longer the dark treatment was, the greater was the chlorophyll loss and membrane ion leakage (Supplementary Fig. S2A, B at JXB online). These results were in line with the decrease in the expression of GhCAB (Supplementary Fig. S2C) and the increase in GhNAP expression (Supplementary Fig. S2D).


A novel NAP member GhNAP is involved in leaf senescence in Gossypium hirsutum.

Fan K, Bibi N, Gan S, Li F, Yuan S, Ni M, Wang M, Shen H, Wang X - J. Exp. Bot. (2015)

Physiological and molecular analysis of GhNAP expression during leaf senescence in cotton. (A–E) Cotton leaves at various developmental stages and GhNAP expression. YL, a young leaf half the size of a fully expanded leaf; NS, a fully expanded, non-senescent leaf; ES, an early senescent leaf, with <50% leaf area yellowing; LS, a late senescent leaf, with >50% leaf area yellowing. Chlorophyll content (B), membrane ion leakage (C), and relative expression of GhNAP (D) and GhCAB (E) at the corresponding stage of (A). Error bars indicate the standard error (n=3). Significant differences between means are represented by different letters. A similar statistical analysis was conducted in the following parts. (F–H) Different parts in the senescing cotton leaf and the relative expression level of GhNAP and GhCAB. B, base; M, middle; T, tip. EF1α was used as the standard control in all qRT-PCR experiments in cotton. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507772&req=5

Figure 2: Physiological and molecular analysis of GhNAP expression during leaf senescence in cotton. (A–E) Cotton leaves at various developmental stages and GhNAP expression. YL, a young leaf half the size of a fully expanded leaf; NS, a fully expanded, non-senescent leaf; ES, an early senescent leaf, with <50% leaf area yellowing; LS, a late senescent leaf, with >50% leaf area yellowing. Chlorophyll content (B), membrane ion leakage (C), and relative expression of GhNAP (D) and GhCAB (E) at the corresponding stage of (A). Error bars indicate the standard error (n=3). Significant differences between means are represented by different letters. A similar statistical analysis was conducted in the following parts. (F–H) Different parts in the senescing cotton leaf and the relative expression level of GhNAP and GhCAB. B, base; M, middle; T, tip. EF1α was used as the standard control in all qRT-PCR experiments in cotton. (This figure is available in colour at JXB online.)
Mentions: The expression of GhNAP was examined in young leaves (YL), non-senescent leaves (NS), early senescent leaves (ES), and late senescent leaves (LS) (Fig. 2A). Consistent with the leaf phenotype, the chlorophyll loss and membrane ion leakage of ES and LS were higher than those of YL and NS (Fig. 2B, C). Moreover, compared with YL and NL, the expression of GhNAP was higher in ES and LS, whereas GhCAB, a negative senescence marker gene, was expressed at a lower level in the same leaves (Fig. 2D, E). During the senescence process (Fig. 2F), more GhNAP expression was detected in the yellow tip (T) than in other parts (Fig. 2G). The present findings were further strengthened by the opposite expression level of GhCAB (Fig. 2H). Furthermore, YL were treated in a dark environment for 7 d. The longer the dark treatment was, the greater was the chlorophyll loss and membrane ion leakage (Supplementary Fig. S2A, B at JXB online). These results were in line with the decrease in the expression of GhCAB (Supplementary Fig. S2C) and the increase in GhNAP expression (Supplementary Fig. S2D).

Bottom Line: Furthermore, the expression of GhNAP was closely associated with leaf senescence.Moreover, the expression of GhNAP can be induced by abscisic acid (ABA), and the delayed leaf senescence phenotype in GhNAPi plants might be caused by the decreased ABA level and reduced expression level of ABA-responsive genes.All of the results suggested that GhNAP could regulate the leaf senescence via the ABA-mediated pathways and was further related to the yield and quality in cotton.

View Article: PubMed Central - PubMed

Affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, PR China.

No MeSH data available.


Related in: MedlinePlus