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Identification of jasmonic acid-associated microRNAs and characterization of the regulatory roles of the miR319/TCP4 module under root-knot nematode stress in tomato.

Zhao W, Li Z, Fan J, Hu C, Yang R, Qi X, Chen H, Zhao F, Wang S - J. Exp. Bot. (2015)

Bottom Line: A total of 263 known miRNAs and 441 novel miRNAs were significantly regulated under RKN stress in the two libraries.Reverse genetics demonstrated that the miR319/TCP4 module affected JA synthetic genes and the endogenous JA level in leaves, thereby mediating RKN resistance.These results suggested that the action of miR319 in serving as a systemic signal responder and regulator that modulated the RKN systemic defensive response was mediated via JA.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.

No MeSH data available.


Related in: MedlinePlus

A scatter plot of differentially expressed known (A) and novel (B) miRNAs. The abscissa represents the expression level of miRNAs in the WT; the ordinate represents the expression level of miRNAs in spr2. Dots appearing in the shaded sections represent differential expression miRNAs. Top left, increased expression; bottom right: reduced expression. (C, D) The number of known and novel differentially expressed miRNAs in KEGG pathways. The corresponding percentage is shown above the column. (This figure is available in colour at JXB online.)
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Figure 2: A scatter plot of differentially expressed known (A) and novel (B) miRNAs. The abscissa represents the expression level of miRNAs in the WT; the ordinate represents the expression level of miRNAs in spr2. Dots appearing in the shaded sections represent differential expression miRNAs. Top left, increased expression; bottom right: reduced expression. (C, D) The number of known and novel differentially expressed miRNAs in KEGG pathways. The corresponding percentage is shown above the column. (This figure is available in colour at JXB online.)

Mentions: To identify the differential expression of known miRNAs from the two libraries for the WT and spr2 strain, clean reads were used to compare known plant miRNA precursors or mature miRNA sequences using miRBase 16.0. Novel miRNAs were predicted according to the precursors’ hairpin secondary structure in Mireap and by using the miRNA/miRNA* criteria. The miRNA expression was considered to be significantly up-regulated or down-regulated with fold changes >2.0 or fold changes < –2.0, respectively, with P≤0.001. In total, 263 known and 441 novel miRNAs were differentially expressed (Supplementary Tables S2, S3 at JXB online). Scatter plots were used to compare the expression of known and novel miRNAs (Fig. 2A, B).


Identification of jasmonic acid-associated microRNAs and characterization of the regulatory roles of the miR319/TCP4 module under root-knot nematode stress in tomato.

Zhao W, Li Z, Fan J, Hu C, Yang R, Qi X, Chen H, Zhao F, Wang S - J. Exp. Bot. (2015)

A scatter plot of differentially expressed known (A) and novel (B) miRNAs. The abscissa represents the expression level of miRNAs in the WT; the ordinate represents the expression level of miRNAs in spr2. Dots appearing in the shaded sections represent differential expression miRNAs. Top left, increased expression; bottom right: reduced expression. (C, D) The number of known and novel differentially expressed miRNAs in KEGG pathways. The corresponding percentage is shown above the column. (This figure is available in colour at JXB online.)
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4507771&req=5

Figure 2: A scatter plot of differentially expressed known (A) and novel (B) miRNAs. The abscissa represents the expression level of miRNAs in the WT; the ordinate represents the expression level of miRNAs in spr2. Dots appearing in the shaded sections represent differential expression miRNAs. Top left, increased expression; bottom right: reduced expression. (C, D) The number of known and novel differentially expressed miRNAs in KEGG pathways. The corresponding percentage is shown above the column. (This figure is available in colour at JXB online.)
Mentions: To identify the differential expression of known miRNAs from the two libraries for the WT and spr2 strain, clean reads were used to compare known plant miRNA precursors or mature miRNA sequences using miRBase 16.0. Novel miRNAs were predicted according to the precursors’ hairpin secondary structure in Mireap and by using the miRNA/miRNA* criteria. The miRNA expression was considered to be significantly up-regulated or down-regulated with fold changes >2.0 or fold changes < –2.0, respectively, with P≤0.001. In total, 263 known and 441 novel miRNAs were differentially expressed (Supplementary Tables S2, S3 at JXB online). Scatter plots were used to compare the expression of known and novel miRNAs (Fig. 2A, B).

Bottom Line: A total of 263 known miRNAs and 441 novel miRNAs were significantly regulated under RKN stress in the two libraries.Reverse genetics demonstrated that the miR319/TCP4 module affected JA synthetic genes and the endogenous JA level in leaves, thereby mediating RKN resistance.These results suggested that the action of miR319 in serving as a systemic signal responder and regulator that modulated the RKN systemic defensive response was mediated via JA.

View Article: PubMed Central - PubMed

Affiliation: Beijing Key Laboratory for Agricultural Application and New Technique, Plant Science and Technology College, Beijing University of Agriculture, Beijing, 102206, China.

No MeSH data available.


Related in: MedlinePlus